Molecular characterization of plasmids encoding CTX-M-15  -lactamases from Escherichia coli strains in the United Kingdom

Karisik, E.; Ellington, Matthew J.; Pike, Rachel; Warren, Richard M.; Livermore, David M.; Woodford, Neil

doi:10.1093/jac/dkl309

Cited by 126 publications

(90 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Similar results were reported from Asia [24,30], Africa [23,25], and Europe [31,32]. In addition to their resistance to thirdand fourth-generation cephalosporins and monobactams, a high percentage of the isolates were also resistant to amoxicillin-clavulanic acid, gentamicin, ciprofloxacin, nalidixic acid, trimethoprimsulfamethoxazole, and tetracycline.…”

Section: Discussionsupporting

confidence: 79%

“…Furthermore, 93.3% of them were MDR. Review of previous studies [6,30,31,33] revealed that these properties are characteristics of the E. coli ST131 clone producing CTX-M-15 (not detected in this work), which characterized by multidrug resistance and coproduction of OXA-1 or TEM-1b β-lactamases as well as aminoglycoside resistance genes aac(3')-IIa and aac(6')-Ib-cr (which also deactivates ciprofloxacin) on transferable plasmids. This widespread nature of this clone all over the world urges us also to try, in the near future, to detect its prevalence among our isolates.…”

Section: Discussionmentioning

confidence: 51%

See 1 more Smart Citation

Distribution of ESBLs among Escherichia coli isolates from outpatients with recurrent UTIs and their antimicrobial resistance

Al-Mayahie

Kuriashy

2016

J Infect Dev Ctries

View full text Add to dashboard Cite

Introduction: Extended-spectrum β-lactamase (ESBL)-producing strains of Enterobacteriaceae are considered to be emerging pathogens. They are a major problem in hospitalized as well as community-based patients. Major outbreaks involving ESBL strains have been reported from all over the world. Recurrent urinary tract infection (UTI) is one of the risk factors for infection with ESBL-producing E. coli in hospitalized and non-hospitalized patients. Methodology: Ninety-one E. coli isolates from outpatients with recurrent UTIs were surveyed phenotypically and genotypically for ESBL production and tested for antimicrobial susceptibility. Results: Of 91 E. coli isolates, 75.8% were resistant to each of cefotaxime and ceftazidime and 74.7% produced ESBLs. CTX-M-type was the most frequent ESBL (accounting for 70.3%), with CTX-M-1 being the only subtype possessed by these isolates. The prevalence of OXA-and SHV-type was 32.9% and 10.9%, respectively. None of the isolates produced TEM β-lactamase. All OXA-type ESBL were produced concomitantly with CTX-M1. Both ESBL producers and non-producers had high resistance to ampicillin followed by trimethoprimsulphamethoxazole, third-generation cephalosporins, and tetracycline. No isolate showed resistance to imipenem and meropenem. In total, resistance rates of ESBL producers were higher than those of ESBL non-producers, as was multidrug resistance (52.7% versus 8.7%, respectively). Conclusions: Our study documented high distribution of ESBLs among E. coli isolates from outpatients with recurrent UTIs, with CTX-M as the predominant ESBL. In the current situation, it is important that antibiotic treatment is be started only after a proper sensitivity report is obtained from the laboratory.

show abstract

Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 51%

Distribution of ESBLs among Escherichia coli isolates from outpatients with recurrent UTIs and their antimicrobial resistance

Al-Mayahie

Kuriashy

2016

J Infect Dev Ctries

View full text Add to dashboard Cite

show abstract

“…Association of the bla CTX-M-15 gene with IncFII replicons has been described in studies conducted in Canada, France, Spain, and the United Kingdom (5,7,8,17,19). Although we observed intercontinental dissemination of 3 major IncFII plasmid scaffolds (A, B, and C) carrying bla CTX-M-15 , similarity >70% among all variants studied and presence of genes also found in pC15-1a, a CTX-M-15 plasmid (GenBank accession no.…”

Section: Discussionsupporting

confidence: 63%

Dissemination of Clonally RelatedEscherichia coliStrains Expressing Extended-Spectrum β-Lactamase CTX-M-15

Coque

Novais

Carattoli

et al. 2008

Emerg. Infect. Dis.

668

469

View full text Add to dashboard Cite

show abstract

“…Furthermore, molecular typing was performed by PCR and sequencing of several antibiotic resistance genes (β-lactamase genes (bla SHV , bla CTX-M , bla TEM ) [2,8], genes of aminoglycosides-modifying enzymes (aac(6')-Ib, aac(3)-II) [3], mobile quinolone resistance genes (qnrA, qnrB and qnrS) [9,10] and quinolone resistance determining regions (QRDRs) of gyrA and parC) [3], by plasmid profile analysis and plasmid RFLP (restriction fragment lenght polymorphism), and by transfer of resistance determinants to Escherichia coli K12 J5-3…”

Section: Methodsmentioning

confidence: 99%

Dissemination of ST274Klebsiella pneumoniaeepidemic clone in newborn and adult hospital settings harbouring SHV-2A or CTX-M-15 type extended spectrum β-lactamases-producing known plasmids

Damjanova¹,

Tóth²,

Kenesei³

et al. 2011

European Journal of Microbiology and Immunology

View full text Add to dashboard Cite

Molecular epidemiology and genetic features of an extended-spectrum β-lactamase (ESBL) producing Klebsiella pneumoniae epidemic clone (KP-EC) with elevated ciprofloxacin MIC (minimum inhibitory concentration) values from multiple nosocomial outbreaks and sporadic cases between 2006 and 2008 in Hungary were investigated.As a result of continuous monitoring of ESBL-producing KP-ECs, 27 isolates collected from five healthcare facilities were selected for macrorestriction profile analysis by PFGE (pulsed field gel electrophoresis). Of these, 12 strains were isolated from adult inpatients, while 15 strains were from newborns. The MIC values for several antibiotics were determined by agar dilution technique. Molecular typing was further performed by PCR (polymerase chain reaction) and sequencing of several antibiotic resistance genes, plasmid profile analysis, transfer of resistance determinants and multilocus sequence typing (MLST).All isolates showed moderate resistance to ciprofloxacin (MICs ranged from 0.5 to 8 mg L -1 ). PFGE revealed the existence of only one genetic cluster defined as EC IV. PstI digestion of plasmid DNA revealed two highly diverse restriction patterns in "adult" and "newborn" isolates corresponding to plasmids from the Hungarian Epidemic Clone and plasmids isolated from a neonatal nosocomial outbreak in 1998, respectively. Sequence analysis of b-lactamase genes from plasmids of 14 selected isolates detected bla SHV-2a in strains isolated exclusively from newborns and bla in strains isolated exclusively from adult inpatients. MLST established that strains of the PFGE cluster belonged to a novel sequence type ST274.ESBL-producing K. pneumoniae isolates belonging to the novel sequence type ST274 appeared in the newborn and adult hospital settings in Hungary and acquired SHV-2a or CTX-M-15 type enzymes, respectively. Thus, a new antimicrobial resistance strategy for successful conformation to distinct hospital settings was found.

show abstract

Molecular characterization of plasmids encoding CTX-M-15 -lactamases from Escherichia coli strains in the United Kingdom

Cited by 126 publications

References 15 publications

Distribution of ESBLs among Escherichia coli isolates from outpatients with recurrent UTIs and their antimicrobial resistance

Distribution of ESBLs among Escherichia coli isolates from outpatients with recurrent UTIs and their antimicrobial resistance

Dissemination of Clonally RelatedEscherichia coliStrains Expressing Extended-Spectrum β-Lactamase CTX-M-15

Dissemination of ST274Klebsiella pneumoniaeepidemic clone in newborn and adult hospital settings harbouring SHV-2A or CTX-M-15 type extended spectrum β-lactamases-producing known plasmids

Contact Info

Product

Resources

About