Molecular characterization of porcine circovirus-like virus P1 in eastern China

Detection of porcine circovirus-like virus P1 in Hebei, China

“…In conclusion, this is the first large‐scale systematic study to detect the P1 virus in Hebei Province, China, and extends our understanding the epidemiology and genotypes of P1. Our results, combined with our previous reports (Wen, Jiao, Zhang et al., ), suggest that P1 is an almost universal virus within the swine herds of central and eastern China. Further studies are required to clarify the evolution and pathogenesis of the P1 virus, including the various mutant strains.…”

“…The total DNA from the sera was extracted with a commercial kit (Tiandz, Inc., China), according to the manufacturer's instructions. The genomic DNA was checked with a previously described method, in which two pairs of primers were used to amplify the complete genome of the P1 virus to ensure the reliability and accuracy of the results (Wen, Jiao, Zhang et al., ). The PCR thermal cycling conditions used were as follows: pre‐denaturation at 98°C for 2 min; 40 cycles of denaturation at 98°C for 10 s, annealing at 56°C for 15 s, extension at 72°C for 5 s and a final extension at 72°C for 5 min.…”

“…The P1 ORF1‐encoded Cap protein sequences were predicted with DNAMAN 5.2.2 (Lynnon). Multiple alignments of the nucleotide sequences and amino acid sequences of P1 strains, including the 12 P1 strains identified in this study and 14 representative P1 strains (Wen et al., a) available in GenBank, were constructed with the Clustal W method, and the phylogenetic relationships were assessed with MEGA 7.0. The genetic variation and nucleotide distance matrices of the complete genomic sequences and the capsid amino acid sequences of the 26 P1 strains were analysed with neighbour‐joining (NJ) and maximum likelihood (ML) trees, respectively.…”

Evidence of porcine circovirus-like virus P1 in piglets with an unusual congenital tremor

“…The three novel P1 virus whole genome sequences generated in this study from brain tissue of piglets with CT were deposited in GenBank under accession numbers MF716583–MF716585. Whole genome sequence identities of the three novel P1 strains from brains and GenBank deposition of P1 representative strains (Wen et al., ) were performed using the DNAstar software. A phylogenetic tree based on the full‐length nucleotide sequences of P1 was generated using the maximum‐likelihood method with 1,000 bootstrap replicates implemented in MEGA 7.0 (http://www.megasoftware.net).…”

Porcine circovirus-like virus P1 in cattle, goats and rabbits in China

“…In May 2016‐January 2017, a surveillance of P1 virus was conducted in Jiangsu Province, China. A total of 300 serum samples, including 120 samples from four cattle farms, 120 samples from three goat farms, and 60 samples from two rabbit farms, were collected and tested for P1 viral DNA with PCR and primers specific for the complete genomic sequence of the P1 virus, as described previously (Wen, Jiao, Zhang, Li et al., ).…”