2012
DOI: 10.1007/s11738-012-1189-2
|View full text |Cite
|
Sign up to set email alerts
|

Molecular characterization of SCARECROW (CsSCR) gene expressed during somatic embryo development and in root of cucumber (Cucumis sativus L.)

Abstract: Somatic embryogenesis (SE) in plants can be used as a model for studying genes engaged in the embryogenic transition of somatic cells. The CsSCARECROW (CsSCR) gene was previously identified among a panel of genes upregulated after the induction of SE in cucumber (Cucumis sativus). The putative CsSCR protein contains conserved GRAS family domains and is extremely similar to AtSCR from Arabidopsis thaliana. SCR proteins are transcription factors involved in root radial patterning and are required for maintenance… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

0
6
0

Year Published

2014
2014
2022
2022

Publication Types

Select...
7

Relationship

1
6

Authors

Journals

citations
Cited by 9 publications
(6 citation statements)
references
References 56 publications
0
6
0
Order By: Relevance
“…The pKGW-RR-MGW-DR5 (DR5::GUS) construct containing the auxin-inducible synthetic promoter DR5, the gusA reporter gene and the pAtUBQ10::DsRED1 cassette as a screenable marker was developed in a previous study (Ilina et al, 2012). To create the pKGW-RR-MGW-pCsSCR (CsSCR::GUS) construct, a 1684 bp fragment of the promoter of the C. sativus SCARECROW (SCR) gene (Wiśniewska et al, 2013) was amplified by PCR using cucumber genomic DNA. The construct was cloned into pUC18-entry8 (Hornung et al, 2005).…”
Section: Design Of Destination Clones For Plant Transformationsmentioning
confidence: 99%
“…The pKGW-RR-MGW-DR5 (DR5::GUS) construct containing the auxin-inducible synthetic promoter DR5, the gusA reporter gene and the pAtUBQ10::DsRED1 cassette as a screenable marker was developed in a previous study (Ilina et al, 2012). To create the pKGW-RR-MGW-pCsSCR (CsSCR::GUS) construct, a 1684 bp fragment of the promoter of the C. sativus SCARECROW (SCR) gene (Wiśniewska et al, 2013) was amplified by PCR using cucumber genomic DNA. The construct was cloned into pUC18-entry8 (Hornung et al, 2005).…”
Section: Design Of Destination Clones For Plant Transformationsmentioning
confidence: 99%
“…In Arabidopsis, the collaboration between the SHR-SCR complex and auxin influx carriers (LAX3 and AUX1) could lead to synergistic effect on primary/lateral root development [77]. In pine and cucumber, relatively high expressing GRAS transcripts, such as SCR and SHR, were measured in non-differentiated proliferating embryogenic cultures and during embryo development [15,78]. In our study, LcGRAS14 in SHR was highly expressed in root, and LcGRAS48 was highly expressed in fruit, indicating that LcGRAS members might function in root and seed development by participating in auxin signal pathway.…”
Section: Discussionmentioning
confidence: 99%
“…Cucumber embryogenic suspension cultures provide an efficient and useful means of studying gene expression during embryogenesis (Malinowski et al 2004;Grabowska et al 2009;Wiśniewska et al 2012Wiśniewska et al , 2013, mainly because there is no need for plant regeneration and de novo induction of somatic embryogenesis. Previously, we described two cucumber XTH genes, CsXTH1 and CsXTH3, which were involved in somatic embryogenesis (Linkiewicz et al 2004;Malinowski et al 2004;Wiśniewska et al 2012).…”
Section: Discussionmentioning
confidence: 99%