MOLECULAR CHARACTERIZATION OF SOLUBLE AND MEMBRANE‐BOUND TREHALASES IN THE COTTON MIRID BUG, <i>Apolygus lucorum</i>

Tan, Yongan; Xiao, Liubin; Sun, Yuhan; Zhao, Jing; Bai, Liang; Xiao, Yingfang

doi:10.1002/arch.21166

Cited by 19 publications

(26 citation statements)

References 28 publications

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“…The trehalase content in the different organs of H. armigera was measured, and the enzyme was found primarily in the midgut, which was consistent with that in other insects (Tan et al, ; Xie, Yang, Dou, & Wang, ). According to the alternation of the three carbohydrates and the related enzymes, all of the three β‐glucosides can inhibit trehalase, and it can also be proven that santonin was the most effective inhibitor in H. armigera .…”

Section: Discussionsupporting

confidence: 81%

“…In insects, trehalase is one of the most important enzymes of carbohydrate metabolism, which is the central part of these organisms’ metabolism. Trehalase is classified into two types: soluble and membrane trehalase (Tan et al, ). This enzyme fuels energy‐demanding synthetic reactions (Beitner & Rivka, ) and provides necessary building blocks for various anabolic pathways (Nakano & Sacktor, ).…”

Section: Introductionmentioning

confidence: 99%

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Effects of toxic β‐glucosides on carbohydrate metabolism in cotton bollworm, Helicoverpa armigera (Hübner)

Lin

et al. 2019

Arch Insect Biochem Physiol

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The purpose of this study was to evaluate the effects of three toxic β-glucosides, phlorizin, santonin, and amygdalin, on carbohydrate metabolism in the cotton bollworm, Helicoverpa armigera (Hübner), when diets mixed with β-glucosides were fed to third-instar larvae. The growth of the larvae was significantly inhibited by exposure to santonin after 96 hr but not obviously affected by phlorizin and amygdalin. The midgut trehalase activities were only 51.7%, 32%, and 42.5% of that of the control after treatment with phlorizin, santonin and amygdalin at 2 mg/ml, respectively. In the hemolymph and fat body, the amount of trehalose decreased in all cases. However, the effects of santonin on the alteration of the glycogen and glucose levels as well as the activities of glycogen phosphorylase, were different than those of the other two β-glucosides. It appears that the three β-glucosides have different influences on the carbohydrate metabolism of cotton bollworm. K E Y W O R D Scarbohydrate metabolism, cotton bollworm, trehalase, β-glucosides

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Section: Discussionsupporting

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Effects of toxic β‐glucosides on carbohydrate metabolism in cotton bollworm, Helicoverpa armigera (Hübner)

Lin

et al. 2019

Arch Insect Biochem Physiol

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“…The mRNA expression profiles of AlTre‐1 and AlTre‐2 across developmental stages (first–fifth‐instar nymph and female adult) were assayed using the method of Tan et al . ().…”

Section: Methodsmentioning

confidence: 97%

“…The relative gene expression levels of AlEcR-B were represented by relative quantification values calculated by the realtime quantitative PCR and the 2 2DDCt method (Livak & Schmittgen, 2001). The mRNA expression profiles of AlTre-1 and AlTre-2 across developmental stages (first-fifth-instar nymph and female adult) were assayed using the method of Tan et al (2014b).…”

Section: Cloning Of the Alecr-b Genementioning

confidence: 99%

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Ecdysone receptor isoform‐B mediates soluble trehalase expression to regulate growth and development in the mirid bug, Apolygus lucorum (Meyer‐Dür)

Tan

Xiao

Zhao

et al. 2015

Insect Molecular Biology

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Ecdysone receptor (EcR) is the hormonal receptor of ecdysteroids and strictly regulates growth and development in insects. However, the action mechanism of EcR is not very clear. In this study, the cDNA of EcR isoform-B was cloned from Apolygus lucorum (AlEcR-B) and its expression profile was investigated. We reduced AlEcR-B mRNA expression using systemic RNA interference in vivo, and obtained knockdown specimens. Examination of these specimens indicated that AlEcR-B is required for nymphal survival, and that reduced expression is associated with longer development time and lower nymphal weight. To investigate the underlying molecular mechanism of the observed suppression effects, we selected trehalase for a detailed study. Transcript encoding soluble trehalase (AlTre-1) was up-regulated by 20-hydroxyecdysone and in agreement with the mRNA expression of AlEcR-B. The expression profile of AlTre-1, soluble trehalase activity and translated protein level in the midgut of surviving nymphs were down-regulated, compared with controls, after the knockdown expression of AlEcR-B. By contrast, membrane-bound trehalase activity, the related gene expression and translated protein level remained at their initial levels. However, trehalose content significantly increased and the glucose content significantly decreased under the same conditions. We propose that AlEcR-B controls normal carbohydrate metabolism by mediating the expression of AlTre-1 to regulate the growth and development in A. lucorum, which provide an extended information into the functions of AlEcR-B.

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The effects of acetamiprid multigeneration stress on metabolism and physiology of Aphis glycines Matsumura (Hemiptera: Aphididae)

Liu

Zhang

et al. 2022

Arch Insect Biochem Physiol

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Aphis glycines Matsumura (Hemiptera: Aphididae) is a major soybean pest that often poses a serious threat to soybean production. In this study, we checked the effects of acetamiprid on redox, energy metabolism, and hormone expression in A. glycines. The LC 50 and LC 30 of acetamiprid were used to treat the fourth instar nymphs in each generation from F0 to F4 to measure the activity of peroxidase, pyruvate kinase, and trehalase using a microassays approach. The peroxidase activity was significantly higher than control when treated with the LC 30 of acetamiprid in F2−F5 generations. The activity of pyruvate kinase was significantly higher, while trehalase activity was substantially lower than control in each generation.Besides, we monitored molting and juvenile hormone expression in soybean aphids using enzyme-linked immunosorbent assay. The juvenile hormone titer of third instar nymphs was significantly higher in the treatment group (F1, F2, F4, and F5), while no effects were noted in the F3 generation. Taken together, the activity of peroxidase and pyruvate kinase in soybean aphid first increased to the peak and then decreased, while the trehalase activity continuously decreased in all generations following exposure to acetamiprid. The juvenile hormone titer was significantly higher, while the molting hormone titer was significantly lower in LC 50 -treated aphids than in control.

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MOLECULAR CHARACTERIZATION OF SOLUBLE AND MEMBRANE‐BOUND TREHALASES IN THE COTTON MIRID BUG, Apolygus lucorum

Cited by 19 publications

References 28 publications

Effects of toxic β‐glucosides on carbohydrate metabolism in cotton bollworm, Helicoverpa armigera (Hübner)

Effects of toxic β‐glucosides on carbohydrate metabolism in cotton bollworm, Helicoverpa armigera (Hübner)

Ecdysone receptor isoform‐B mediates soluble trehalase expression to regulate growth and development in the mirid bug, Apolygus lucorum (Meyer‐Dür)

The effects of acetamiprid multigeneration stress on metabolism and physiology of Aphis glycines Matsumura (Hemiptera: Aphididae)

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