Molecular characterization of sulfate‐reducing bacteria in a New England salt marsh

Bahr, Michele; Crump, Byron C.; Klepac‐Ceraj, Vanja; Teske, Andreas; Sogin, Mitchell L.; Hobbie, John E.

doi:10.1111/j.1462-2920.2005.00796.x

Cited by 107 publications

(89 citation statements)

References 68 publications

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“…Clone sequences in cluster G were closely related to an environmental sequence retrieved from a uranium mill tailing site (Chang et al 2001). Sequences from this cluster were also recovered from freshwater or brackish environments such as estuarine sediments (Bahr et al 2005, Kondo et al 2004, Leloup et al 2006) and a wetland (Castro et al 2002), but not marine environments (Dhillon et al 2003, Thomsen et al 2001). Members of the Desulfobulbaceae family can use alternative electron acceptors leading to sulphate and can disproportionate sulphur oxianions while Desulfobulbus is known to be able to grow by fermentation of lactate or ethanol and CO 2 without sulphate.…”

mentioning

confidence: 85%

“…This cluster also contains environmental dsrA sequences retrieved from estuarine sediments (INOC-DSR3, INOC-DSR26, VN4, VN11), a mesophilic sulphide-rich spring (ZDSR2), a salt marsh (PIM02A05) and a deep-sea hydrothermal vent chimney (INDO-40). These environmental clones were reported to be closely related to the genera Desulfococcus, Desulfonema and Desulfosarcina (Bahr et al 2005, Elshahed et al 2003, Joulian et al 2001, Leloup et al 2006, Nakagawa et al 2004. Cluster D was related to the genus Desulfosarcina.…”

Section: Diversity Of Srb Based On Dsramentioning

confidence: 99%

“…5). Sequences from this group were abundantly recovered from the surface sediment of Aarhus Bay, Denmark (Thomsen et al 2001), a New England salt marsh (Bahr et al 2005) and the Colne estuary, UK (Kondo et al 2004). Desulfonema and Desulfosarcina species are marine organisms that require NaCl to grow, while Desulfococcus species are freshwater organisms but also grow well in brackish and marine media (Widdel & Bak 1992).…”

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confidence: 99%

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Abundance and diversity of sulphate-reducing bacterioplankton in Lake Suigetsu, a meromictic lake in Fukui, Japan

Kondo¹,

Osawa²,

Mochizuki³

et al. 2006

Plankton Benthos Res

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Abstract:The depth distribution of sulphate-reducing bacteria (SRB) in the water column of a meromictic lake, Lake Suigetsu, Fukui, Japan was investigated using quantitative competitive PCR targeting the gene coding for portions of the a-subunit of dissimilatory sulphite reductase (dsrA). The total bacterial cell density (DAPI count) was 5Ϫ13ϫ10 6 cells mL Ϫ1 in the water column of the lake with maximum abundance occurring at the oxic-anoxic interface layer. SRBwere not detected in oxic surface water using competitive PCR. SRB were found in the anoxic waters below the oxycline ranging from 10 4 to 10 5 cells mL Ϫ1 , accounting for 0.3-8.9% of the total bacteria. The SRB cell densities were higher than previously estimated using the most-probable-number (MPN) method. Sequencing of the cloned PCR product of dsrA showed the existence of different SRB groups in the anoxic water. The majority of the dsrA sequences were associated with the Desulfosarcina-Desulfococcus-Desulfonema group and members of the Desulfobulbaceae family.Other dsrA clones belonged to the Desulfomicrobium and Desulfovibrio species as well as to a deeply branched group in the dsrA tree with no representatives from previously isolated SRB groups. These SRB species appear to be important for the sulphur and carbon cycle in the anoxic waters of Lake Suigetsu.

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confidence: 85%

Section: Diversity Of Srb Based On Dsramentioning

confidence: 99%

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confidence: 99%

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Abundance and diversity of sulphate-reducing bacterioplankton in Lake Suigetsu, a meromictic lake in Fukui, Japan

Kondo¹,

Osawa²,

Mochizuki³

et al. 2006

Plankton Benthos Res

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“…These organisms completely oxidize a diverse range of organic compounds, including acetate and other short chain fatty acids, to CO2. Similar sequences have also been recovered from other marine and freshwater environments 1,16,41) . Desulfosarcina has been reported to be numerically abundant in sediment of the German Wadden Sea 25) .…”

Section: Phylogenetic Analysesmentioning

confidence: 51%

Spatial Dynamics of Sulphate-reducing Bacterial Compositions in Sediment along a Salinity Gradient in a UK Estuary

et al. 2007

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The diversity of sulphate-reducing bacteria (SRB) was investigated in sediments along environmental gradients in the River Colne estuary, Essex, UK. DNA samples were collected from four sites; marine-dominated (Alresford Creek), brackish (the Hythe), predominantly freshwater (East Hill Bridge) and freshwater (the Weir) between September 2001 and May 2002. SRB community composition was assessed by PCR amplification, cloning and sequencing of part of the α subunit of dissimilatory sulphite reductase (dsrA) using directly extracted sediment DNA. The majority of the dsrA sequences were associated with members of the Desulfobacteraceae family, the Desulfobulbaceae family and a deeply branched group in the dsrA tree with no cultured representatives. There was some evidence of a salinity-related distribution within both the Desulfobacteraceae and Desulfobulbaceae groups. Clones related to Desulfotomaculum of the xenologues Firmicutes and a phylogenetically distinct Colne group 3 were detected only at the freshwater East Hill Bridge and Weir sites. Conversely, clones related to an uncultured group (Colne group 1) were found only at the marine and brackish sites. A statistical analysis of composition revealed that dsrA sequences from the marine-dominated Alresford Creek and the brackish site at the Hythe were not significantly different from each other (P>0.05), but were significantly different from those of the freshwater-dominated East Hill Bridge and the Weir (P<0.05). The sequences from East Hill Bridge and the Weir were not significantly different from each other (P>0.05). The data presented show a complex distribution of SRB along the estuary with some evidence to support the idea that salinity and sulphate concentrations are an important factor in determining SRB community structure.Key words: dissimilatory sulphite reductase gene, environmental gradients, estuarine sediment, sulphate reduction Sulphate reduction is an important process involved in both the global carbon and sulphur cycles. This process can dominate anaerobic terminal mineralisation of organic matter in high-sulphate sediments, degrading up to 50% of all organic matter in coastal marine sediments 12) and plays a minor but still important role in low-sulphate sediments 26,38) . Rates of sulphate reduction vary in relation to temperature and electron donor and acceptor concentrations with the availability of sulphate an important factor in the control of the rate of sulphate reduction. Sulphate concentrations range from approximately 25 mM in full-strength seawater to <0.2 mM in freshwater 7) . Freshwater-adapted sulphatereducing bacteria (SRB) have a greater affinity for sulphate (K m =5-68 µM) than marine-adapted SRB (K m =200 µM) 8,37) , allowing them to continue sulphate reduction at much lower sulphate concentrations than marine SRB. Physiological differences between freshwater-and marine-adapted SRB may be reflected in differences in species or phylogenetic compositions of SRB communities.The Colne estuary is a small, muddy, macrotidal (3 to 5...

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“…SRB are common in environments including marine sediments, salt marshes, deepsea hydrothermal vents and contaminated aquifers (Jorgensen et al 1992;Bahr et al 2005;Wu et al 2009). To the best of our knowledge, this is the first report of the presence of SRB in coral skeletons.…”

Section: Discussionmentioning

confidence: 99%

Sulfate-reducing bacteria in the skeleton of the massive coral <i>Goniastrea aspera</i> from the great barrier reef

Yuen

Yamazaki

Baird

et al. 2013

Galaxea, Journal of Coral Reef Studies

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Coral skeletons harbor diverse assemblages of endolithic microorganisms that often have a profound influence on the ecology and physiology of the coral host.While the cyanobacterial and eukaryotic microalgal com ponents of these endolithic assemblages are well char acterized, information on the other components remains scarce. Here, we characterize the endolithic microbial assemblages in the skeleton of the massive coral Gonia strea aspera in both oligotrohic and eutrophic environ ments. In addition to the green bands typically found in massive coral skeletons, multiple black bands were observed in skeletons from oligotrophic waters. Sequence analysis of the dsrB (dissimilatroy sulfite reductase beta subunit) gene revealed that colonies with black bands harbor a diverse assemblage of sulfatereducing bacteria (SRB). In contrast, SRB were not detected in skeletons from eutrophic waters. We conclude that increases in nutrients may have an important effect on the microbial assemblages residing within coral skeletons.

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Molecular characterization of sulfate‐reducing bacteria in a New England salt marsh

Cited by 107 publications

References 68 publications

Abundance and diversity of sulphate-reducing bacterioplankton in Lake Suigetsu, a meromictic lake in Fukui, Japan

Abundance and diversity of sulphate-reducing bacterioplankton in Lake Suigetsu, a meromictic lake in Fukui, Japan

Spatial Dynamics of Sulphate-reducing Bacterial Compositions in Sediment along a Salinity Gradient in a UK Estuary

Sulfate-reducing bacteria in the skeleton of the massive coral <i>Goniastrea aspera</i> from the great barrier reef

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