Molecular characterization of the complete genome of a street rabies virus isolated in China

Ming, Pinggang; Du, Jinyan; Tang, Qing; Yan, Jiaxin; Nadin-Davis, Susan A.; Li, Hao; Tao, Xiaoyan; Huang, Ying; Hu, Rongliang; Liang, Guodong

doi:10.1016/j.virusres.2009.02.014

Cited by 28 publications

(16 citation statements)

References 42 publications

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“…Under Indian scenario, it is clear that the spatial spread of rabies viruses is limited by geographical boundaries or topographical barriers and not by political boundaries. Similar findings on grouping of viruses based on geography was previously reported for isolates from Indian sub-continent [20][21][22]28] and also other part of the world [11,13,19]. This finding indicates that rough idea for place of origin of rabies virus can be made using G gene based sequence analysis.…”

Section: Discussionsupporting

confidence: 86%

“…It is reported that the rabies vaccine failure may occur due to genetic difference between local virus isolates and vaccine strains in use and it could be overcome by developing vaccine strains from locally circulating viruses [1,16]. Therefore, the best vaccine strain is one that is as closely related as possible to the street viruses circulating within the specific geographical location [3,16,19]. Nucleoprotein gene based phylogenetic analysis revealed the similar findings as for G gene.…”

Section: Discussionmentioning

confidence: 82%

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Molecular and immunogenic characterization of BHK-21 cell line adapted CVS-11 strain of rabies virus and future prospect in vaccination strategy

et al. 2015

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Development of a cost effective quality vaccine is a key issue in rabies control programme in developing countries. With this perspective, in the present study, challenge virus standard (CVS)-11 strain of rabies virus was adapted to grow in BHK-21 cells, characterized, compared with other viruses including global vaccine strains and field isolates from Indian subcontinent and China at molecular level. This cell adapted virus was evaluated for the production of cost effective veterinary vaccine. The maximum virus titre achieved was 10 7 fluorescent focus unit (FFU)/mL at 10th passage level. There was no nucleotide difference in the nucleoprotein (N) and glycoprotein (G) genes after adaptation in cell line. Phylogenetic analysis showed that adapted virus was grouped with global vaccine strains, closest being with other CVS strains but distinct from the Indian field isolates. Global vaccine strains including cell adapted CVS-11 virus have 83-87 % identity at nucleotide level of G gene with Indian field viruses. Growth kinetics of cell culture adapted virus showed that the optimum virus titer (around 10 7 FFU/mL) could be obtained at around 48 h post infection by cocultivation method using 0.1 multiplicity of infection inoculums at 37°C. These findings can be used for up scaling of vaccine production. The protective efficacy of test vaccine produced using 10 6.95 FFU/mL cell culture harvest showed 1.17 IU/mL relative potency by NIH test. Further, adapted virus was found to be suitable for use in rapid fluorescent focus inhibition test.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 82%

Molecular and immunogenic characterization of BHK-21 cell line adapted CVS-11 strain of rabies virus and future prospect in vaccination strategy

et al. 2015

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“…Some characteristic mutations were found in the glycoprotein gene of SRV9, namely Arg333Gly, Gly53Glu, His192Arg and Thr311Ala (Ming et al, 2009;Wei et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…1 day p.e., respectively . SRV9 is a Chinese attenuated RV strain, which was subcloned from SADB19 and had been previously reported by many researchers (Geue et al, 2008;Ming et al, 2009;Jiao et al, 2011;. Since there is, however, still just few data available related to its characteristics in prophylactic and p.e.…”

Section: Introductionmentioning

confidence: 99%

Efficiency of live attenuated and inactivated rabies viruses in prophylactic and post exposure vaccination against the street virus strain

Huang¹,

Ahmad²,

Duan³

et al. 2015

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Summary. -Rabies remains an enigmatic and widely discussed global infectious disease and causes an increasing number of deaths. The currently used highly effective prophylactic and post exposure (p.e.) vaccination depends solely upon inexpensive, effective and safe vaccines to counteract the spread of the disease. In this study, the potential of an attenuated Chinese rabies vaccine (SRV9) strain in prophylactic and p.e. vaccination against the street strain of rabies virus (RV) was evaluated in mice. Prophylactic vaccination consisting of one intramuscular (i.m.) dose of SRV9 protected 100% of mice from intracerebral (i.c.) challenge with a lethal dose of the street virus. The latter was detected in the brain of mice at day 6 post challenge by RT-PCR. Post exposure vaccination was performed at days 1, 2, 3, 4, 5 and 6 post infection (p.i.) with either SRV9 or inactivated rabies vaccine. The survival rates after i.m. inoculation of SRV9 at the indicated days were 70%, 50%, 30%, 20%, 10%, and 0%, respectively; the corresponding survival rates for the inactivated rabies vaccine were 30%, 20%, 10%, 0%, 0%, and 0%, respectively. However, 100%, 90%, 70%, 50%, 20%, 10%, and 10% of mice survived after i.c. inoculation of SRV9 at the indicated days. The increased permeability of the blood-brain barrier and the infiltration of CD19+ B cells into the central nervous system after i.c. inoculation of SRV9 are regarded as prerequisites for the clearance of the street virus. The obtained data suggest that SRV9 is a promising candidate for prophylactic and p.e. vaccination against rabies infection and that it exhibits a potential for the control of rabies in China.Keywords: attenuated rabies vaccine; inactivated rabies vaccine; post exposure vaccination * Corresponding author. E-mail: zhrei98@163.com; phone: +86-15044130833. The first three authors contributed equally to this work. Abbreviations: BBB = blood-brain barrier; CNS = central nervous system; i.c. = intracerebral(ly); i.m. = intramuscular(ly); p.i. = post inoculation/infection; p.e. = post exposure; PEP = post exposure prophylaxis; PrEP = pre exposure prophylaxis; RV = rabies virus

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“…Recent reports indicated that the number of N-glycosylation sites in G protein is one of the determinants of the pathogenicity of street viruses (12)(13)(14). Most G proteins of street viruses have two N-glycosylation sites at Asn 37 and Asn 319 , whereas fixed viruses have additional glycosylation sites on G protein at Asn 158 , Asn 204 , or Asn 247 (12,13,15,16) (Fig. 1).…”

Section: Introductionmentioning

confidence: 99%

Association between RABV G Proteins Transported from the Perinuclear Space to the Cell Surface Membrane and N-Glycosylation of the Sequon Asn<sup>204</sup>

et al. 2015

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SUMMARY:In this study, G proteins of the rabies virus (RABV) Kyoto strain were detected in the cytoplasm but not distributed at the cell membrane of mouse neuroblastoma (MNA) cells. G proteins of CVS-26 were detected in both the cell membrane and perinuclear space of MNA cells. We found that Nglycosylation of street RABV G protein by the insertion of the sequon Asn 204 induced the transfer of RABV G proteins to the cell surface membrane. Fixed RABV budding from the plasma membrane has been found to depend not only on G protein but also on other structural proteins such as M protein. However, the differing N-glycosylation of G protein could be associated with the distinct budding and antigenic features of RABV in street and fixed viruses. Our study of the association of N-glycan of G protein at Asn 204 with the transport of RABV G protein to the cell surface membrane contributes to the understanding of the evolution of fixed virus from street virus, which in turn would help for determine the mechanism underlying RABV budding and enhanced host immune responses.

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Molecular characterization of the complete genome of a street rabies virus isolated in China

Cited by 28 publications

References 42 publications

Molecular and immunogenic characterization of BHK-21 cell line adapted CVS-11 strain of rabies virus and future prospect in vaccination strategy

Molecular and immunogenic characterization of BHK-21 cell line adapted CVS-11 strain of rabies virus and future prospect in vaccination strategy

Efficiency of live attenuated and inactivated rabies viruses in prophylactic and post exposure vaccination against the street virus strain

Association between RABV G Proteins Transported from the Perinuclear Space to the Cell Surface Membrane and N-Glycosylation of the Sequon Asn<sup>204</sup>

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