Molecular characterization of the Sinorhizobium meliloti nlp D gene

Streit, Wolfgang R.; Hofmann, Kai; Liebl, Wolfgang

doi:10.1007/s002030000205

Cited by 3 publications

(1 citation statement)

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“…RSc1206 is predicted to encode a protein sharing sequence homology with outer membrane lipoproteins NlpDs (see below), and this gene is therefore considered to be an ortholog of nlpD and renamed accordingly. NlpD is implicated to be involved in E. coli cell wall formation and maintenance (Lange and Hengge-Aronis 1994) and daughter cell separation (Uehara et al 2010), in Sinorhizobium meliloti survival in the stationary phase (Streit et al 2000), and in Yersinia pestis cell separation and the development of Yersiniae plagues (Tidhar et al 2009). However, function of NlpDs in bacterial pathogenesis under stress environments, such as establishing an infection in less susceptible hosts, has not been elucidated so far.…”

Section: Introductionmentioning

confidence: 99%

Ralstonia solanacearum nlpD (RSc1206) contributes to host adaptation

et al. 2012

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Ralstonia solanacearum is a soil-borne xylem-inhibited pathogen and causes a deadly wilting disease on a wide range of crops. This bacterium can also colonize many weeds and native plants without necessarily causing symptoms, leading to devastating epidemiological consequences; however, little is known about the biology. A R. solanacearum mutant harbouring a transposon insertion in the annotated gene RSc1206 was previously shown to retain virulence in tomato but displayed reduced virulence in model weed host Arabidopsis. In this study, we investigated the function of RSc1206 in bacterial pathogenesis in weed hosts. RSc1206 encodes a putative protein that is homologous to E. coli NlpD, and the organization of RSc1206-related gene cluster is mostly conserved among representative gram-negative bacteria analyzed. We therefore designate it R. solanacearum gene nlpD. Microscopic data revealed that the nlpD mutant had defects in cell separation and envelope integrity. An increased sensitivity to hydrophobic toxic chemicals confirmed that the cell integrity of the nlpD mutant was damaged. In addition, the activity of the nlpD mutant in biofilm formation and motility was reduced. However, enzymatic activity and tobacco pathogenesis assays revealed that this mutant functioned normally in the Type II and III secretion systems. Trans-mutation and trans-complementation analyses further verified that disruption of nlpD function was responsible for the observed defects. Our study provides evidence that NlpD contributes to R. solanacearum cell integrity and pathogenesis in weed host Arabidopsis.

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Section: Introductionmentioning

confidence: 99%

Ralstonia solanacearum nlpD (RSc1206) contributes to host adaptation

et al. 2012

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Structure of Thermotoga maritima Stationary Phase Survival Protein SurE

et al. 2001

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Transposon Mutagenesis Reveals Differential Pathogenesis of Ralstonia solanacearum on Tomato and Arabidopsis

Lin¹,

Chou²,

Wang³

et al. 2008

MPMI

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Ralstonia solanacearum causes a deadly wilting disease on a wide range of crops. To elucidate pathogenesis of this bacterium in different host plants, we set out to identify R. solanacearum genes involved in pathogenesis by screening random transposon insertion mutants of a highly virulent strain, Pss190, on tomato and Arabidopsis thaliana. Mutants exhibiting various decreased virulence levels on these two hosts were identified. Sequence analysis showed that most, but not all, of the identified pathogenesis genes are conserved among distinct R. solanacearum strains. A few of the disrupted loci were not reported previously as being involved in R. solanacearum pathogenesis. Notably, a group of mutants exhibited differential pathogenesis on tomato and Arabidopsis. These results were confirmed by characterizing allelic mutants in one other R. solanacearum strain of the same phylotype. The significantly decreased mutants' colonization in Arabidopsis was found to be correlated with differential pathogenesis on these two plants. Differential requirement of virulence genes suggests adaptation of this bacterium in different host environments. Together, this study reveals commonalities and differences of R. solanacearum pathogenesis on single solanaceous and nonsolanaceous hosts, and provides important new insights into interactions between R. solanacearum and different host plants.

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Molecular characterization of the Sinorhizobium meliloti nlp D gene

Cited by 3 publications

References 8 publications

Ralstonia solanacearum nlpD (RSc1206) contributes to host adaptation

Ralstonia solanacearum nlpD (RSc1206) contributes to host adaptation

Structure of Thermotoga maritima Stationary Phase Survival Protein SurE

Transposon Mutagenesis Reveals Differential Pathogenesis of Ralstonia solanacearum on Tomato and Arabidopsis

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