Molecular Characterization of Tomato leaf curl Palampur virus and Pepper leaf curl betasatellite Naturally Infecting Pumpkin (Cucurbita moschata) in India

Jaiswal, Namrata; Saritha, R. K.; Datta, D. V.; Singh, Mahesh Kumar; Dubey, R. S.; B., A.; Rai, Mathura

doi:10.1007/s13337-011-0022-7

Cited by 37 publications

(11 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similarly, previous report showed that trans-replication of betasatellites in plants inoculated with TYLCCV and TbCSV has been well proved through agro-inoculation [50]. Further, yellow vein mosaic disease of pumpkin was caused by Tomato leaf curl Palampur virus, is associated with Pepper leaf curl betasatellite [51], and in cotton, single betasatellite molecule will support for pathogenicity of multiple viruses [10].…”

Section: Discussionmentioning

confidence: 59%

Molecular Evidence for Association of Tobacco Curly Shoot Virus and a Betasatellite with Curly Shoot Disease of Common Bean (Phaseolus vulgaris L.) from India

Venkataravanappa¹

2012

J Plant Pathol Microb

View full text Add to dashboard Cite

A new strain (FB01) of Tobacco curly shoot virus (TbCSV) showing curly shoot symptoms on common bean plants from Varanasi, Uttar Pradesh state of India was characterized. The analysis of the whole genome sequence and individual ORFs of this virus indicated that it is very closely related (sequence similarity of 89.1-94.5%) to the TbCSV infecting solanaceous and other weed crops in India and China. This was well supported by phylogenetic analysis with close clustering of the virus isolate with TbCSV. The absence of DNA-B and association of virus with betasatellite confirmed it as a monopartite begomovirus. The betasatellite identified here shared highest (53.9-93.9%) sequence identity with tomato leaf curl betasatellite. Further, putative recombination events were recognized within the virus sequence, suggesting that the virus is a recombinant and evolved from recombination of Tobacco curly shoot virus, Munbean yellow mosaic virus, Tomato leaf curl Jodhpur virus, Tobacco leaf curl Yunnan virus and Ageratum enation virus like ancestors. For betasatellite, the putative recombination events were recognized within the sequence, were interspecific. The new recombinant betasatellite was derived from recombination between Croton yellow vein mosaic betasatellite and Tomato yellow leaf curl China betasatellite, as the foremost parents in its evolution. The virus was transmitted by whiteflies as well as sap, and not by seed.

show abstract

Section: Discussionmentioning

confidence: 59%

Molecular Evidence for Association of Tobacco Curly Shoot Virus and a Betasatellite with Curly Shoot Disease of Common Bean (Phaseolus vulgaris L.) from India

Venkataravanappa¹

2012

J Plant Pathol Microb

View full text Add to dashboard Cite

show abstract

“…As compared to ToLCNDV, the ToLCPMV is a recently identified begomovirus, reported from bitter cucumber, Rumex sp, tomato, pumpkin, squash, cucumber, bitter gourd, common bean, water melon, chilies, and muskmelon from India and Iran (Namrata et al 2010;Heydarnejad et al 2009;Esmaeili et al 2015). In Pakistan ToLCPMV was first detected in 2007 infecting Bitter gourd (Momordica charantia L.), showing typical symptoms of the disease including chlorosis, leaf crumpling, vein thickening, and stunting of plants (Ali et al 2010).…”

Section: Discussionmentioning

confidence: 99%

“…The common viruses involved in the yellow mosaic infection of cucurbits are Cucumber green mottle mosaic virus (CGMMV), Zucchini yellow mosaic virus (ZYMV), Watermelon mosaic virus (WMV), Papaya ring spot virus (PRSV) and Squash leaf curl China virus (SLCCNV) (Ali et al 2004;Tahir et al 2010). Tomato leaf curl Palampur virus (ToLCPMV) has been investigated as an infectious agent of leaf curl disease in cucurbits, tomato, cucumber and muskmelon (Namrata et al 2010;Heydarnejad et al 2009;Esmaeili et al 2015;Malik et al 2011). This virus is relatively new introduction in agro ecological zone of Pakistan.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization and phylogenetic analysis of tomato leaf curl Palampur virus, a bipartite begomovirus, associated with Cucumis sativus L. in Pakistan

et al. 2019

View full text Add to dashboard Cite

Leaf samples of Cucumis Sativus L. (C. sativus) (Family; Cucurbitaceae) showing vein thickening, mild leaf curling and leaf enations were collected from the farmer's field. Amplification of the full-length viral molecules was performed through rolling circle amplification (RCA). Cloning of the full-length viral molecules was done through standard cloning procedure followed by sequencing. Sequence similarity analysis and phylogenetic studies showed that the virus associated with leaf curling and enations in C. sativus was a bipartite begomovirus, where DNA-A and DNA-B showed highest nucleotide sequence homology of 98% and 97% to tomato leaf curl Palampur virus (ToLCPMV) from India. Attempts to isolate betasatellites and alphasatellites through PCR using RCA product as template, did not result in any amplification. A maximum likelihood phylogenetic tree grouped DNA-A and B components with other isolates from India. SDT was used to find the pairwise identity scores of different sequences of ToLCPMV present in the database. Phylogenetic analysis showed that sequences of ToLCPMV DNA-A and B components in this study share high degree of homology with existing viruses and are isolates of ToLCPMV-India. Infectious molecules of both components (Accessions, MG252783 and MG252784, respectively) were constructed for infectivity analysis to fulfill the Koch's postulate. Infectivity analysis revealed that ToLCPMV DNA-A is infectious to model host plant Nicotiana benthamiana and viral accumulation was confirmed through Southern blot analysis. Accumulation of DNA-B was confirmed through PCR. Infectivity analysis was also conducted using the original host, C. sativus, but plants were unable to survive the agroinoculation. To our knowledge this is the first report of ToLCPMV associated with C. sativus L. in Pakistan.

show abstract

“…The specific begomoviruses that are prevalent in the disease hot spot we have used in this study are unknown or were not determined in Tomato lines (F 6 ) derived from FLA478-6-1-11 9 CLN1621E this study. However, it is likely that the population of begomoviruses include both monopartite and bipartite begomoviruses (Chakraborty et al 2003;Namrata et al 2010;Venkataravanappa et al 2014). The two virus isolates used in the present study represent begomovirus species that are predominant in India (Tiwari et al 2010;Jyothsna et al 2013).…”

Section: Discussionmentioning

confidence: 99%

Marker assisted selection of Ty-2 and Ty-3 carrying tomato lines and their implications in breeding tomato leaf curl disease resistant hybrids

et al. 2015

View full text Add to dashboard Cite

Tomato leaf curl virus disease caused by whitefly-transmitted begomoviruses is a serious tomato production constraint in tropical and subtropical regions of the world. Use of genetic resistance for diseases caused by begomoviruses is an important component of sustainable disease management approaches. To date, six introgressions (Ty genes) derived from different tomato wild species that confer resistance to tomato leaf curl disease have been identified. Among these, dominant Ty-2 and partially dominant Ty-3 are important for the development of resistant hybrids because of their gene action. In the present study we report marker assisted selection of tomato lines carrying Ty-2 and Ty-3 genes and the effect of combining these in tomato hybrids using agroinoculation. The response of lines and hybrids with the Ty-2 gene indicated ineffectiveness of Ty-2 to infection by a bipartite tomato leaf curl virus. The Ty-3 gene showed dosage effect with partial resistance in heterozygous state. However, the level of resistance in hybrids with the combination of Ty-2 and Ty-3 genes was higher and similar to homozygous Ty-3 plants.The Ty-2 gene in either homozygous or heterozygous state enhanced the level of resistance of plants with heterozygous Ty-3. Thus, tomato hybrid breeding programs can be benefited by using tomato lines carrying Ty-2 and Ty-3 resistance genes. The combined results of evaluation of horticultural performance, field resistance and agroinoculation tests demonstrated that the set of tomato lines developed in this study could be useful resources for achieving higher level of resistance in tomato hybrids.

show abstract

Molecular Characterization of Tomato leaf curl Palampur virus and Pepper leaf curl betasatellite Naturally Infecting Pumpkin (Cucurbita moschata) in India

Cited by 37 publications

References 20 publications

Molecular Evidence for Association of Tobacco Curly Shoot Virus and a Betasatellite with Curly Shoot Disease of Common Bean (Phaseolus vulgaris L.) from India

Molecular Evidence for Association of Tobacco Curly Shoot Virus and a Betasatellite with Curly Shoot Disease of Common Bean (Phaseolus vulgaris L.) from India

Molecular characterization and phylogenetic analysis of tomato leaf curl Palampur virus, a bipartite begomovirus, associated with Cucumis sativus L. in Pakistan

Marker assisted selection of Ty-2 and Ty-3 carrying tomato lines and their implications in breeding tomato leaf curl disease resistant hybrids

Contact Info

Product

Resources

About