Molecular Characterization of Tospovirus Transmitting Thrips Populations from India

Meena, Rang Lal; Ramasubram, T.; Venkatesan, Swaminathan; Mohankumar, S.

doi:10.3844/ajbbsp.2005.167.172

Cited by 10 publications

(7 citation statements)

References 15 publications

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“…This pest damages its host by feeding on the young leaves, buds, and fruits resulting in feeding scars, color distortions, and stunted growth (Seal et al 2005, 2006). Scirtothrips dorsalis is the only Scirtothrips species known to be a vector of certain Tospoviruses which include Groundnut bud necrosis virus , Peanut chlorotic fanspot virus , and Peanut yellow spot virus (Whitfield et al 2005; Meena et al 2005; Moritz et al 2000). This species of thrips has a habitat that is widespread ranging from temperate to tropical climatic regions.…”

Section: Introductionmentioning

confidence: 99%

Development of a Ribosomal DNA ITS2 Marker for the Identification of the Thrips,Scirtothrips dorsalis

Farris¹,

Ruiz‐Arce

Ciomperlik³

et al. 2010

Journal of Insect Science

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The thrips Scirtothrips dorsalis Hood (Thysanoptera: Thripidae) is an invasive pest that poses a significant economical threat to U.S. agriculture and trade. In this study, DNA sequence data and polymerase chain reaction (PCR) were utilized to develop a molecular diagnostic marker for S. dorsalis. The DNA sequence variation from the internal transcribed spacer 2 (ITS2) region of nuclear ribosomal DNA (rDNA) was analyzed from various thrips species, including S. dorsalis. A primer set and polymerase chain reaction cycling parameters were designed for the amplification of a single marker fragment of S. dorsalis ITS2 rDNA. Specificity tests performed on ten thrips species, efficacy tests performed on fifteen S. dorsalis populations, and tests on primer sensitivity and robustness all demonstrated the diagnostic utility of this marker. This diagnostic PCR assay provides a quick, simple, and reliable molecular technique to be used in the identification of S. dorsalis.

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Section: Introductionmentioning

confidence: 99%

Development of a Ribosomal DNA ITS2 Marker for the Identification of the Thrips,Scirtothrips dorsalis

Farris¹,

Ruiz‐Arce

Ciomperlik³

et al. 2010

Journal of Insect Science

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“…Only a few hundred of the 5,500 identified thrips species are pests (Brunner et al 2002), and of these, only 14 are documented virus vectors (Riley et al 2011). Chilli thrips Scirtothrips dorsalis is both a virus vector (Chu et al 2001; Meena et al 2005; Gopal et al 2010) and a pest of many crops around the world including tea (Saha & Mukhopadhyay 2013), mango (Aliakbarpour & Md. Rawi 2012; Choi et al 2013), roses (Hegde et al 2011; Mannion et al 2013), and citrus (Gao et al 2012; Hyun et al 2012).…”

mentioning

confidence: 99%

Estimating Bacterial Diversity inScirtothrips dorsalis(Thysanoptera: Thripidae) via Next Generation Sequencing

Dickey¹,

Trease²,

Jara-Cavieres³

et al. 2014

Florida Entomologist

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The last 2 decades have produced a better understanding of insect-microbial associations and yielded some important opportunities for insect control. However, most of our knowledge comes from model systems. Thrips (Thysanoptera: Thripidae) have been understudied despite their global importance as invasive species, plant pests and disease vectors. Using a culture and primer independent next-generation sequencing and metagenomics pipeline, we surveyed the bacteria of the globally important pest, Scirtothrips dorsalis Hood. The most abundant bacterial phyla identified were Actinobacteria and Proteobacteria and the most abundant genera were Propionibacterium, Stenotrophomonas, and Pseudomonas. A total of 189 genera of bacteria were identified. The absence of any vertically transferred symbiont taxa commonly found in insects is consistent with other studies suggesting that thrips primarilly acquire resident microbes from their environment. This does not preclude a possible beneficial/intimate association between S. dorsalis and the dominant taxa identified and future work should determine the nature of these associations.

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“…Earlier reports suggested that thrips like S. dorsalis (German et al, 1992;Meena et al, 2005), T. palmi (Lakshmi et al, 1995;Meena et al, 2005;Reddy et al, 1992) and F. schultzei (Meena et al, 2005) were observed as putative vectors for GBNV, whereas T. tabaci could transmit Tomato spotted wilt virus (TSWV) (Wijkamp et al, 1995). In NCBI few entries were available related to Tusothrips sumatrensis and T. apicatus.…”

Section: Resultsmentioning

confidence: 99%

Thrips vectors of tospoviruses on tomato in South India

Aravintharaj¹,

Asokan²

2022

ije

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The present study was undertaken to determine distribution of thrips species from major tomato growing regions in South India (Tamil Nadu, Karnataka, Maharashtra, Telangana, Andhra Pradesh). The thrips species were identified using molecular techniques employing universal barcoding primer (mt-COI). These revealed the presence of six species viz., Thrips palmi Karny, Scirtothrips dorsalis Hood, Thrips tabaci Lindeman, Frankliniella schultzei (Trybom), Thrips apicatus Priesner and Tusothrips sumatrensis (Karny). Of these, T. palmi was found to be predominant in all the states surveyed followed by S. dorsalis, F. schultzei and T. tabaci.

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Molecular Characterization of Tospovirus Transmitting Thrips Populations from India

Cited by 10 publications

References 15 publications

Development of a Ribosomal DNA ITS2 Marker for the Identification of the Thrips,Scirtothrips dorsalis

Development of a Ribosomal DNA ITS2 Marker for the Identification of the Thrips,Scirtothrips dorsalis

Estimating Bacterial Diversity inScirtothrips dorsalis(Thysanoptera: Thripidae) via Next Generation Sequencing

Thrips vectors of tospoviruses on tomato in South India

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