Molecular characterization of trimethoprim resistance in<i>Shigella sonnei</i>in Sicily

Agodi, Antonella; Jones, Clive; Threlfall, E. J.; D'Angelo, Maria; Marranzano, Marina

doi:10.1017/s0950268800047610

Cited by 9 publications

(3 citation statements)

References 30 publications

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“…In a study conducted in Sicily the type V DHFR was shown to be the most frequently isolated of the DHFRs in an outbreak of trimethoprim resistant Shigella sp. It was, however, thought that the strain had been introduced in immigrants from Sri Lanka [47]. In a study conducted in Finland, 4 or 5 Shigella carrying the dhfrV gene probably originated in Sri Lanka [48].…”

Section: Discussionmentioning

confidence: 99%

Trimethoprim resistant dihydrofolate reductases in normal faecal flora isolated in India

Tait

Amyes

1994

Epidemiol. Infect.

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SUMMARYA high incidence of resistance to trimethoprim has been shown in the normal faecal flora in a population in south India. The dihydrofolate reductase (dhfr) genes mediating transferable resistance to trimethoprim have been identified.Unusually, in this study, the dhfrV was shown to be the predominant resistance gene (dhfrV 50 % of transconjugants, dhfrla 30%), the dhfrlb was also detected being distinguished from the dhfrV by an oligo-probe. However, when nontransferable resistance was considered, the dhfrIa was the most prevalent of the dhfrs identified. All those plasmids harbouring the dhfrla were shown to possess Tn7. All the plasmids that probed positive for the dhfrV and the dhfrlb were shown to be associated with the integrase of the Tn2l-like transposons, but 8 of the dhfrV genes were not associated with the Tn2l resolvase. The dhfrIV was shown to be present in all seven plasmids that produced low level trimethoprimresistance. The dhfrV, first characterized in Sri Lanka, would seem to have a local distribution in this region of Asia but is distinguishable from the dhfrlb only by the use of an oligo-probe.

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Section: Discussionmentioning

confidence: 99%

Trimethoprim resistant dihydrofolate reductases in normal faecal flora isolated in India

Tait

Amyes

1994

Epidemiol. Infect.

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“…Επίσης, το γονίδιο αυτό εντοπίστηκε και στην Ταϊβάν, σε στελέχη Εντεροβακτηριακών, σε ποσοστό 10,4%, µε επικρατέστερο το dfrΑ1 µε ποσοστό 45,4% [175]. Στην Ευρώπη βρέθηκε για πρώτη φορά σε στελέχη Shigella sonnei στη Σικελία της Ιταλίας, όπου και θεωρηθήκε ότι σχετίζεται µε την παρουσία ταξιδιωτών από τη Σρι Λάνκα [254]. Έκτοτε έχει εντοπιστεί και στη Μ. Βρετανία σε στελέχη που αποµονώθηκαν από δείγµατα ούρων, όπου ο τύπος dfrΑ5 απαντά σε ποσοστό 10-25%, ενώ στην Φινλανδία το αντίστοιχο ποσοστό είναι 0-0,5% [206,207,248,253].…”

Section: μεταβίβαση αντοχής στην τριµεθοπρίµη µε σύζευξηunclassified

“…Στη Μ. Βρεττανία και συγκεκριµένα στην περιοχή του Nottingham, η συχνότητα εµφάνισης του IIIa παρουσιάζει µεγάλες χρονολογικές διακυµάνσεις, µε το µεγαλύτερο ποσοστό 20% να παρατηρείται κατά το χρονικό διάστηµα 1987-1988, και το χαµηλότερο 1% το 1978-1983[224,253]. Ο τύπος IIIb ανιχνεύθηκε για πρώτη φορά σε ανθεκτικά στελέχη S. sonnei στις Ηνωµένες Πολιτείες, ενώ στη συνέχεια εντοπίστηκε και στη Χαβάη[227].Τύπος IV: το γονίδιο IV έχει εντοπιστεί µόνο στην Ινδία, ενώ σε περιοχές όπως η Μ. Βρεττανία, η Ιταλία και η Ταϊβάν πειράµατα υβριδισµού για την ανίχνευσή του ήταν αρνητικά[175,207,228,252,253,254]. παρατηρούνται διαφορές µεταξύ των διαφόρων γεωγραφικών περιοχών καθώς και διακυµάνσεις ακόµη και µέσα στον ευρωπαϊκό χώρο.…”

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Διερεύνηση Μηχανισμών Αντοχής Εντεροβακτηριακών Στην Τριμεθοπρίμη

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CitrobacterCitrobacter freundii Citrobacter (diversus) koseri Citrobacter amanolaticus Edwardsiella Edwardsiella tarda Enterobacter Enterobacter aerogenes Enterobacter cloacae Enterobacter agglomerans group (Pantoea agglomerans) Enterobacter gergoviae Enterobacter sakazaki Enterobacter amnigenus Enterobacter (cancerogenous) taylorae Escherichia Escherichia coli Hafnia Hafnia alvei Klebsiella Klebsiella pneumoniae Klebsiella oxytoca Klebsiella ozaenae Morganella Morganella morganii subsp. morganii Proteus Proteus mirabilis Proteus vulgaris Proteus penneri Providencia Providencia rettgeri Providencia stuartii Salmonella Salmonella, all serotypes Serratia Serratia marcescens Serratia liquefaciens group Shigella Shigella dysenteriae (group A) Shigella flexneri (group B) Shigella boydii (group C) Shigella sonnei (group D) Yersinia Yersinia pestis Yersinia enterocolitica supsp. Enterocolitica Yersinia frederiksenii Yersinia intermedia Yersinia pseudotuberculosis Budvicia Budvicia aquatica Buttiauxella Buttiauxella agrestis Buttiauxella noackia Cedecea Cedecea davisae Cedecea lapagei Cedecea neteri Citrobacter Citrobacter braaki Citrobacter farmeri Citrobacter gilleni Citrobacter murliniaei Citrobacter sedlakii Citrobacter werkmanii Citrobacter youngae Edwardsiella Edwardsiella hoshinae Enterobacter Enterobacter asburiae Enterobacter hormaechei Enterobacter intermedium Enterobacter kobei Erwinia Erwinia spp.

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Molecular characterization of trimethoprim resistance in salmonellas isolated in Sicily, 1985–1988

Agodi

Marranzano

Jones³

et al. 1995

Eur J Epidemiol

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The occurrence of trimethoprim (Tp) resistance in salmonellas isolated from humans and water samples in Sicily between 1985 and 1988 has been investigated and the Tp resistance mechanisms have been further characterized on the basis of hybridization with probes for the dihydrofolate reductase (DHFR) genes types I, II, IV and V. Of 765 strains examined, high level (> 1000 mg/l) resistance to Tp was identified in 23 strains (3%). In 22 of these strains, such resistance was associated with resistance to sulphonamides. Six serovars with Tp-resistant strains were identified, Salmonella typhimurium (14 strains), S. enteridis (2), S. agona (2), S. mbandaka (2), S. virchow (2), S. indiana (1). In all strains with high level Tp resistance, resistance to this antimicrobial was plasmid-encoded, in most strains by plasmids with MWs ranging from 70-100 MDa. On the basis of restriction endonuclease analysis, four different categories of Tp resistance plasmids were identified in Tp-resistant strains of S. typhimurium. Hybridization with the DHFR I probe was observed in three strains of Tp-resistant S. typhimurium and two strains of Tp-resistant S. enteritidis; in contrast, in none of the strains tested was there any detectable hybridization with the probes for DHFR types II, IV and V. It is concluded that the DHFR type I resistance mechanism, common in Tp-resistant enterobacteria in many European countries, is relatively uncommon in Tp-resistant salmonellas isolated in Sicily. Furthermore, the DHFR V resistance mechanism, previously identified in strains of Shigella sonnei isolated in Sicily and associated with travellers from Sri Lanka, has not yet appeared in salmonellas in Sicily.

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Molecular characterization of trimethoprim resistance inShigella sonneiin Sicily

Cited by 9 publications

References 30 publications

Trimethoprim resistant dihydrofolate reductases in normal faecal flora isolated in India

Trimethoprim resistant dihydrofolate reductases in normal faecal flora isolated in India

Διερεύνηση Μηχανισμών Αντοχής Εντεροβακτηριακών Στην Τριμεθοπρίμη

Molecular characterization of trimethoprim resistance in salmonellas isolated in Sicily, 1985–1988

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