Molecular characterization of two ethylene response factor genes in sweetpotato that respond to stress and activate the expression of defense genes in tobacco leaves

Kim, Yun Hee; Jeong, Jae Cheol; Park, Sung Yong; Lee, Haeng Soon; Kwak, Sang Soo

doi:10.1016/j.jplph.2012.03.002

Cited by 25 publications

(19 citation statements)

References 36 publications

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“…Ethylene response factors enhanced salt tolerance in plants [ 49 , 50 ], including transgenic plants [ 50 – 52 ]. Interestingly, two sweetpotato ERF genes, IbERF1 and IbERF2 , appear to be key for controlling up-regulation of various defense genes involved in abiotic stress tolerance and pathogenic resistance [ 53 ]. IbERF1 and IbERF2 expression was found induced in leaves within 2 hours of treatment with 100 mM NaCl; it was also up-regulated under dehydration-, chemical-, and pathogenic-stress treatments.…”

Section: Resultsmentioning

confidence: 99%

Transcriptome Profiling of Beach Morning Glory (Ipomoea imperati) under Salinity and Its Comparative Analysis with Sweetpotato

et al. 2016

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The response and adaption to salt remains poorly understood for beach morning glory [Ipomoea imperati (Vahl) Griseb], one of a few relatives of sweetpotato, known to thrive under salty and extreme drought conditions. In order to understand the genetic mechanisms underlying salt tolerance of a Convolvulaceae member, a genome-wide transcriptome study was carried out in beach morning glory by 454 pyrosequencing. A total of 286,584 filtered reads from both salt stressed and unstressed (control) root and shoot tissues were assembled into 95,790 unigenes with an average length of 667 base pairs (bp) and N50 of 706 bp. Putative differentially expressed genes (DEGs) were identified as transcripts overrepresented under salt stressed tissues compared to the control, and were placed into metabolic pathways. Most of these DEGs were involved in stress response, membrane transport, signal transduction, transcription activity and other cellular and molecular processes. We further analyzed the gene expression of 14 candidate genes of interest for salt tolerance through quantitative reverse transcription PCR (qRT-PCR) and confirmed their differential expression under salt stress in both beach morning glory and sweetpotato. The results comparing transcripts of I. imperati against the transcriptome of other Ipomoea species, including sweetpotato are also presented in this study. In addition, 6,233 SSR markers were identified, and an in silico analysis predicted that 434 primer pairs out of 4,897 target an identifiable homologous sequence in other Ipomoea transcriptomes, including sweetpotato. The data generated in this study will help in understanding the basics of salt tolerance of beach morning glory and the SSR resources generated will be useful for comparative genomics studies and further enhance the path to the marker-assisted breeding of sweetpotato for salt tolerance.

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Section: Resultsmentioning

confidence: 99%

Transcriptome Profiling of Beach Morning Glory (Ipomoea imperati) under Salinity and Its Comparative Analysis with Sweetpotato

et al. 2016

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“…However, the functions of most AP2/ERFs in sweetpotato are largely unknown. Previously, sweetpotato IbERF1 and IbERF2 were reported to respond to abiotic stress and pathogen infection, and can activate the transcription of defense‐related genes in tobacco ( Nicotiana L.; Kim, Jeong, Park, Lee, & Kwak, 2012). A sweetpotato AP2/ERF gene IbRAP2‐12 was shown to regulate salt and drought tolerance in Arabidopsis (Yan et al., 2019).…”

Section: Introductionmentioning

confidence: 99%

Identification, expression analysis, and functional characterization of salt stress‐responsive genes of AP2/ERF transcription factors in sweetpotato

et al. 2020

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The AP2/ERF transcription factors (TFs) regulate various processes of plant growth, development, and response to environmental stimuli. Although they have been extensively documented in many plants, little is known about stress responsive APETALA2/ethylene response factor (AP2/ERF) genes in sweetpotato (Ipomoea batatas L.). In this study, 20 putative AP2/ERF genes, named IbERF1, IbERF2, and IbERF5–IbERF23 were identified based on the salt‐treated RNA‐seq data from two sweetpotato cultivars with different salt tolerance. Phylogenetic analysis and sequence characterization identified that all the screened IbERF proteins contained a conserved AP2 domain, which belonged to dehydration‐responsive element binding proteins (DREB; 7 members) or ERF (13 members) subfamily. The systematic expression analysis of 12 mainly salt‐induced IbERFs by quantitative real‐time polymerase chain reaction (qRT‐PCR) revealed that most IbERFs were remarkably increased by multiple abiotic stresses, such as NaCl, dehydration, and cold stress. Furthermore, varying degrees of upregulation were also detected in the expression of most IbERFs when treated with hormone abscisic acid, jasmonic acid, and salicylic acid. The expression profiles of IbERFs corroborate their involvement in diverse biological processes. In addition, the IbERF5, which was exclusively upregulated by the treatments, was identified as a nuclear protein without transcriptional activation activity. Collectively, the data of stress responsive IbERFs will provide valuable knowledge for further functional studies of IbERFs in sweetpotato stress tolerance.

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“…The expression of the two ERF genes of sweet potato (Ipomoea batatas), IbERF1 and IbERF2, in N. tabacum leaves resulted in increased transcript levels of many genes such as the CuZnSOD and the CAT genes (Kim et al 2012). Hwang et al (2012) also reported that DREB2C plays an important role in promoting oxidative stress tolerance in Arabidopsis.…”

Section: Introductionmentioning

confidence: 99%

The StDREB1 transcription factor is involved in oxidative stress response and enhances tolerance to salt stress

Bouaziz

Jbir

Charfeddine

et al. 2014

Plant Cell Tiss Organ Cult

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We have shown previously that the potato dehydration responsive element binding (StDREB1) transcription factor plays an important role in regulating and improving salt and drought stress-response genes in potato plant. To further characterize StDREB1 involvement in stress response, we focuse here on the investigation of the StDREB1 target genes by an Electrophoretic mobility shift assay. The data obtains indicated that the StDREB1 protein can bind to both GCC and DRE boxes in the promoter sequence of target genes, suggesting that this transcription factor may play a key role in the response to abiotic-and biotic-stresses by the activation of the DRE-and GCCmediated signaling pathways. In a second step, since some DREB factors were related to the oxidative stress response, we showed that H 2 O 2 treatment led to a significant increase of StDREB1 expression in wild-type potato plants. Moreover, the analysis of the oxidative stress response of StDREB1 transgenic potato plants revealed lower levels of H 2 O 2 and malondialdehyde than wild-type control plants submitted in vitro to salt stress. An increase in the antioxidant enzyme activities including superoxide dismutase (SOD) and catalase was also observed in StDREB1 plants. In addition, an enhanced expression of the Cu/Zn SOD gene was noticed in these StDREB1 transgenic plants, cultivated under salt stress conditions. These results suggest that StDREB1 plays an essential role in the regulation of stress-response by regulating the oxidative stress response. The involvement of this transcription factor in the activation of osmoprotectant synthesis was also confirmed.

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Molecular characterization of two ethylene response factor genes in sweetpotato that respond to stress and activate the expression of defense genes in tobacco leaves

Cited by 25 publications

References 36 publications

Transcriptome Profiling of Beach Morning Glory (Ipomoea imperati) under Salinity and Its Comparative Analysis with Sweetpotato

Transcriptome Profiling of Beach Morning Glory (Ipomoea imperati) under Salinity and Its Comparative Analysis with Sweetpotato

Identification, expression analysis, and functional characterization of salt stress‐responsive genes of AP2/ERF transcription factors in sweetpotato

The StDREB1 transcription factor is involved in oxidative stress response and enhances tolerance to salt stress

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