Molecular Characterization, Structural Modeling, and Evaluation of Antimicrobial Activity of Basrai Thaumatin-Like Protein against Fungal Infection

Abstract

A thaumatin-like protein gene from Basrai banana was cloned and expressed in Escherichia coli. Amplified gene product was cloned into pTZ57R/T vector and subcloned into expression vector pET22b(+) and resulting pET22b-basrai TLP construct was introduced into E. coli BL21. Maximum protein expression was obtained at 0.7 mM IPTG concentration after 6 hours at 37°C. Western blot analysis showed the presence of approximately 20 kDa protein in induced cells. Basrai antifungal TLP was tried as pharmacological agent against fungal disease. Independently Basrai antifungal protein and amphotericin B exhibited their antifungal activity against A. fumigatus; however combined effect of both agents maximized activity against the pathogen. Docking studies were performed to evaluate the antimicrobial potential of TLP against A. fumigatus by probing binding pattern of antifungal protein with plasma membrane ergosterol of targeted fungal strain. Ice crystallization primarily damages frozen food items; however addition of antifreeze proteins limits the growth of ice crystal in frozen foods. The potential of Basrai TLP protein, as an antifreezing agent, in controlling the ice crystal formation in frozen yogurt was also studied. The scope of this study ranges from cost effective production of pharmaceutics to antifreezing and food preserving agent as well as other real life applications.

Purification and characterization of a thaumatin-like protein-1 with polyphenol oxidase activity found in Prunus mume

“…This similar to the TLP-1 previously reported in many other plant sources, such as banana (22.1 kDa), 17 Pinus sylvestris seedlings (24.6 kDa), 18 banana pulp (20 kDa). 19 All TLP-1s isolated to date including isolated in this study possess molecular weights, existing a consistent trend, are above 20 kDa. The differences between these results might be due to the different of plant materials.…”

The Secreted Protein MoHrip1 Is Necessary for the Virulence of Magnaporthe oryzae

“…Their expression is often induced by microbial infection and pathogenic elicitors, osmotic stress, wounding and plant hormones [44]. The antifungal activity of PR5 is well-documented no matter in the form of heterologous expressed protein [45,46] or transgenic plants [47]. Meanwhile, they can mediate the signal transduction by interaction with their receptors or ligands [44].…”

LncRNA109897-JrCCR4-JrTLP1b forms a positive feedback loop to regulate walnut resistance against anthracnose caused by Colletotrichum gloeosporioides

“…Meanwhile, pathogenesis-related (PR) proteins act as essential components of the plant innate immune response to play an important role in the defense system [ 15 ]. Among the 17 PR protein families, PR5 family proteins named as thaumatin-like proteins (TLPs), which have been studied to confer antifungal activity in plants through inhibiting mycelial growth and spore germination [ 16 , 17 ]. Overexpression of TaTLP1 can significantly improve wheat resistance to leaf rust induced by Puccinia triticina and common root rot induced by Bipolaris sorokiniana [ 18 ].…”