Molecular characterizations of <i>Vibrio parahaemolyticus</i>
 in seafood from the Black Sea, Turkey

Gülel, Göknur Terzi; Martínez-Urtaza, Jaime

doi:10.1111/lam.12579

Cited by 13 publications

(11 citation statements)

References 29 publications

(52 reference statements)

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“…Results are good agreement with the previous published reports showing the presence of tlh positive V. parahaemolyticus in many sea food samples ( Letchumanan et al, 2014 ). Consistent with this Terzi Gulel and Martinez-Urtaza (2016) identified and confirmed many strains of V. parahaemolyticus using tlh virulence marker. We further noticed that among all the identified V. parahaemolyticus isolates, only 25.0% ( n = 2) were positive for trh .…”

Section: Resultssupporting

confidence: 84%

Occurrence and Identification of Pathogenic Vibrio Contaminants in Common Seafood Available in a Chinese Traditional Market in Qingdao, Shandong Province

Song

Zang

et al. 2020

Front. Microbiol.

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The investigation of the causative agents for foodborne diseases and subsequent development of preventive steps to control the outbreak and related economic loss is the basic goal and priority of a rational food safety program. The entero-pathogenic Vibrio spp., which are Gram negative bacteria inhabiting estuarine ecosystems, are the major cause of foodborne illness associated with the consumption of raw or undercooked contaminated seafood or shellfish. To survey the Vibrio contamination in sea snails (Neptunea cumingi Crosse and Busycon canaliculatu), a total of 20 samples were collected from traditional market, at Qingdao city in Shandong province, China and analyzed for Vibrio species contamination. Presumptive-positive colonies grown on a specific Vibrio agar-based medium were picked and identified by the VITEK TM. Vibrio alginolyticus, V. parahaemolyticus, and V. vulgaris were isolated and identified in 11, 8, and 2 seafood samples, respectively. Among the 8 isolates of V. parahaemolyticus. The V. parahaemolyticus isolates were further tested for the tdh, trh, and tlh virulence factors. All the V. parahaemolyticus isolates were tlh-positive, however, all of them were tdh-negative. Interestingly 2 V. parahaemolyticus isolates were positive for trh virulence factor. These results indicated that there is a high incidence of V. alginolyticus and V. parahaemolyticus in sea snails. Therefore, food safety regulations for fishery auction markets should be established to control these species in addition to other Vibrio pathogenic contaminants. Our study provides the first evidence for the prevalence of Vibrio spp. in sea snail samples from traditional market in the Qingdao province of China.

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Section: Resultssupporting

confidence: 84%

Occurrence and Identification of Pathogenic Vibrio Contaminants in Common Seafood Available in a Chinese Traditional Market in Qingdao, Shandong Province

Song

Zang

et al. 2020

Front. Microbiol.

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“…Similarly, Vibrio isolates recovered from West Sumatran rivers and shrimp farms in Sri Lanka were void of these virulence genes [ 47 , 48 ]. A low prevalence (0% tdh and 4.2% trh ) was also recorded from 96 seafood and water samples in Turkey [ 8 ]. Although clinical cases of V. parahaemolyticus are associated with increased detection of these genes, the continuous report of pathogenic V. parahaemolyticus in the last few years and the fact that it is the most commonly encountered Vibrio species implicated in seafood infection is an indication that other virulence factors may exist [ 49 , 50 , 51 ].…”

Section: Discussionmentioning

confidence: 99%

“…Only toxigenic strains of O1 and O139 V. cholerae are associated with cholera illness; however, other strains belonging to non-O1/non-O139 have been associated with sporadic outbreaks of diarrhoea through the ingestion of contaminated seafood [ 4 ]. V. parahaemolyticus is one of the leading causes of seafood-associated diarrhoeal infection in many countries including the United States, Asian and other developing countries [ 5 , 6 , 7 , 8 , 9 ]. It is one of the frequent causative agents of gastroenteritis, caused by the consumption of raw, undercooked seafood.…”

Section: Introductionmentioning

confidence: 99%

“…The expression of these genes is controlled by the toxR regulon, in response to in vivo stimuli [ 20 ]. The thermostable direct hemolysin ( tdh ), in particular, as well as the thermostable direct hemolysin-related gene ( trh ) are major contributors to the pathogenicity of V. parahaemolyticus [ 8 , 21 ], owing to its biological activities, including its cytotoxic and enterotoxic effects [ 10 ]. The virulence of V. vulnificus is mainly coded for by the virulence-correlated gene ( vcg ).…”

Section: Introductionmentioning

confidence: 99%

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Occurrence of Virulence Genes Associated with Human Pathogenic Vibrios Isolated from Two Commercial Dusky Kob (Argyrosmus japonicus) Farms and Kareiga Estuary in the Eastern Cape Province, South Africa

Fri

Ndip

Njom

et al. 2017

IJERPH

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Background: Seafood-borne Vibrio infections, often linked to contaminated seafood and water, are of increasing global public health concern. The aim of this study was to evaluate the prevalence of human pathogenic vibrios and their associated virulence genes isolated from fish and water samples from 2 commercial dusky kob farms and Kareiga estuary, South Africa. Methods: A total of 200 samples including dusky kob fish (n = 120) and seawater (n = 80) were subjected to Vibrio screening on thiosulfate-citrate-bile salts-sucrose agar (TCBS). Presumptive isolates were confirmed and delineated to V. cholerae, V. parahaemolyticus, V. vulnificus, and V. fluvialis by PCR. Various pathogenic gene markers were screened: V. parahaemolyticus (trh and tdh), V. vulnificus (vcgE and vcgC) and V. fluvialis (stn, vfh, hupO, vfpA). Restriction Fragment Length Polymorphism (RFLP) of the vvhA gene of V. vulnificus strains was performed to determine the associated biotypes. Results: Total Vibrio prevalence was 59.4% (606/1020) of which V. fluvialis was the most predominant 193 (31.85%), followed by Vibrio vulnificus 74 (12.21%) and V. parahaemolyticus 33 (5.45%). No V. cholerae strain was detected. One of the V. parahaemolyticus strains possessed the trh gene 7 (9.46%) while most (91.9%; 68/74) V. vulnificus isolates were of the E-type genotype. V. fluvialis virulence genes detected were stn (13.5%), hupO (10.4%) and vfpA (1.0%). 12.16% (9/74) of V. vulnificus strains exhibited a biotype 3 RFLP pattern. Conclusions: This is the first report of potentially pathogenic vibrios from healthy marine fish in the study area, and therefore a public health concern.

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“…Additionally, it should be noted that the number of V. parahaemolyticus infections has increased and their reach widened globally during recent years (Nair et al 2007 ; Powell et al 2013 ). Scientists are currently investigating the conditions that might be fostering this spread and increase (Kaneko and Colwell 1975 ; Martinez-Urtaza et al 2016 ) so that it might be halted, but in the meantime, early detection is important to seafood consumers in Europe, Asia and the US (Terzi Gulel and Martinez-Urtaza 2016 ).…”

Section: Introductionmentioning

confidence: 99%

A simple, portable, electrochemical biosensor to screen shellfish for Vibrio parahaemolyticus

et al. 2017

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An earlier electrochemical mechanism of DNA detection was adapted and specified for the detection of Vibrio parahaemolyticus in real samples. The reader, based on a screen printed carbon electrode, was modified with polylactide-stabilized gold nanoparticles and methylene blue was employed as the redox indicator. Detection was assessed using a microprocessor to measure current response under controlled potential. The fabricated sensor was able to specifically distinguish complementary, non-complementary and mismatched oligonucleotides. DNA was measured in the range of 2.0 × 10−8–2.0 × 10−13 M with a detection limit of 2.16 pM. The relative standard deviation for 6 replications of differential pulse voltammetry (DPV) measurement of 0.2 µM complementary DNA was 4.33%. Additionally, cross-reactivity studies against various other food-borne pathogens showed a reliably sensitive detection of the target pathogen. Successful identification of Vibrio parahaemolyticus (spiked and unspiked) in fresh cockles, combined with its simplicity and portability demonstrate the potential of the device as a practical screening tool.

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Molecular characterizations of Vibrio parahaemolyticus in seafood from the Black Sea, Turkey

Cited by 13 publications

References 29 publications

Occurrence and Identification of Pathogenic Vibrio Contaminants in Common Seafood Available in a Chinese Traditional Market in Qingdao, Shandong Province

Occurrence and Identification of Pathogenic Vibrio Contaminants in Common Seafood Available in a Chinese Traditional Market in Qingdao, Shandong Province

Occurrence of Virulence Genes Associated with Human Pathogenic Vibrios Isolated from Two Commercial Dusky Kob (Argyrosmus japonicus) Farms and Kareiga Estuary in the Eastern Cape Province, South Africa

A simple, portable, electrochemical biosensor to screen shellfish for Vibrio parahaemolyticus

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