Molecular cloning and characterisation of in vitro immune response against astacin-like metalloprotease Ace-MTP-2 from Ancylostoma ceylanicum

Bąska, Piotr; Wiśniewski, Marcin; Krzyżowska, Małgorzata; Długosz, Ewa; Zygner, Wojciech; Górski, Piotr; Wędrychowicz, H.

doi:10.1016/j.exppara.2013.01.006

Cited by 30 publications

(22 citation statements)

References 41 publications

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“…However, gut associated metalloproteases are not the only class of hookworms metalloproteases. Hookworms express astacin like metalloproteases (Zhan et al 2002;Mendez et al 2005;Feng et al 2007;Bąska et al 2013). Immunization with Ace-MTP-1 and Ac-MTP-1 resulted in no statistical significant worm burden reduction but diminished fecal egg count was observed after immunization with Ace-MTP-1 .…”

Section: Discussionmentioning

confidence: 99%

Ancylostoma ceylanicum metalloprotease 6 DNA vaccination induces partial protection against hookworm challenge infection

Wiśniewski

Jaros

Bąska

et al. 2013

Acta Parasitologica

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Hookworms are blood feeding intestinal nematodes that infect more than 500 million people and cause iron deficiency anemia. Infected children suffer from physical and cognitive growth retardation. Because of potential anthelminthic drug resistance, the need for vaccine development is urgent. Numerous antigens have been tested in animal models as vaccines against hookworm infection, but there is no effective human vaccine. We cloned a cDNA encoding Ancylostoma ceylanicum metalloprotease 6 (Acemep-6). Ace-MEP-6 is a protein with a predicted molecular mass of 101.87 kDa and based on computational analysis it is very likely to be engaged in food processing via hemoglobin digestion. Groups of hamsters were immunized with an Ace-mep-6 cDNA vaccine, either once or three times. Animals that were administered one dose developed high resistance (80%, p < 0.01) against challenge infection, whereas triple immunization resulted in no worm burden reduction. These results suggest that DNA vaccines can be powerful tools in ancylostomiasis control, although the mechanisms through which protection is conferred remain unclear.

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Section: Discussionmentioning

confidence: 99%

Ancylostoma ceylanicum metalloprotease 6 DNA vaccination induces partial protection against hookworm challenge infection

Wiśniewski

Jaros

Bąska

et al. 2013

Acta Parasitologica

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“…Members of the astacin family have been considered to maintain blood flow to the lesion site through hydrolysis of fibrinogen and fibronectin [78,79] leading to local haemorrhage [80]. It has been hypothesised that astacins have digestive and anticoagulation roles, also inactivating prey/host vasoactive peptides [76,81] to maintain hostparasite relationships [82][83][84][85]. However, sea lice astacin is expressed in tegument glands associated with probable roles in lubrication of integument as opposed to feeding [86].…”

Section: Discussionmentioning

confidence: 99%

Identification of proteins from the secretory/excretory products (SEPs) of the branchiuran ectoparasite Argulus foliaceus (Linnaeus, 1758) reveals unique secreted proteins amongst haematophagous ecdysozoa

et al. 2020

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Background: It is hypothesised that being a blood-feeding ectoparasite, Argulus foliaceus (Linnaeus, 1758), uses similar mechanisms for digestion and host immune evasion to those used by other haematophagous ecdysozoa, including caligid copepods (e.g. sea louse). We recently described and characterised glands associated with the feeding appendages of A. foliaceus using histological techniques. The work described in the present study is the first undertaken with the objective of identifying and partially characterising the components secreted from these glands using a proteomic approach.Methods: Argulus foliaceus parasites were sampled from the skin of rainbow trout (Oncorhynchus mykiss), from Loch Fad on the Isle of Bute, Scotland, UK. The proteins from A. foliaceus secretory/excretory products (SEPs) were collected from the supernatant of artificial freshwater conditioned with active adult parasites (n = 5-9 per ml; n = 560 total). Proteins within the SEPs were identified and characterised using LC-ESI-MS/MS analysis. Data are available via ProteomeXchange with identifier PXD016226.

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“…Strategies included examining IgE responses in the sera from populations in countries with endemic STH infections, mutating the antigenic epitopes recognized by host IgE and using bioinformatics tools that can screen for allergenicity ( 212 , 219 ). Adult hookworms suck blood from damaged vessels in the gut mucosa and digest hemoglobin using haemoglobinases ( 220 , 221 ). Since neutralization of these critical enzymes would result in starvation of the parasites, leading to parasite death, these antigens were selected for the development of hookworm vaccines ( 49 , 222 ).…”

Section: Soil-transmitted Helminth Vaccines: Are We Getting Closer?mentioning

confidence: 99%

Soil-Transmitted Helminth Vaccines: Are We Getting Closer?

Zawawi

Else

2020

Front. Immunol.

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Parasitic helminths infect over one-fourth of the human population resulting in significant morbidity, and in some cases, death in endemic countries. Despite mass drug administration (MDA) to school-aged children and other control measures, helminth infections are spreading into new areas. Thus, there is a strong rationale for developing anthelminthic vaccines as cost-effective, long-term immunological control strategies, which, unlike MDA, are not haunted by the threat of emerging drug-resistant helminths nor limited by reinfection risk. Advances in vaccinology, immunology, and immunomics include the development of new tools that improve the safety, immunogenicity, and efficacy of vaccines; and some of these tools have been used in the development of helminth vaccines. The development of anthelminthic vaccines is fraught with difficulty. Multiple lifecycle stages exist each presenting stage-specific antigens. Further, helminth parasites are notorious for their ability to dampen down and regulate host immunity. One of the first significant challenges in developing any vaccine is identifying suitable candidate protective antigens. This review explores our current knowledge in lead antigen identification and reports on recent pre-clinical and clinical trials in the context of the soil-transmitted helminths Trichuris , the hookworms and Ascaris . Ultimately, a multivalent anthelminthic vaccine could become an essential tool for achieving the medium-to long-term goal of controlling, or even eliminating helminth infections.

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Molecular cloning and characterisation of in vitro immune response against astacin-like metalloprotease Ace-MTP-2 from Ancylostoma ceylanicum

Cited by 30 publications

References 41 publications

Ancylostoma ceylanicum metalloprotease 6 DNA vaccination induces partial protection against hookworm challenge infection

Ancylostoma ceylanicum metalloprotease 6 DNA vaccination induces partial protection against hookworm challenge infection

Identification of proteins from the secretory/excretory products (SEPs) of the branchiuran ectoparasite Argulus foliaceus (Linnaeus, 1758) reveals unique secreted proteins amongst haematophagous ecdysozoa

Soil-Transmitted Helminth Vaccines: Are We Getting Closer?

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