1999
DOI: 10.1074/jbc.274.3.1621
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Molecular Cloning and Characterization of a Mitogen-activated Protein Kinase-associated Intracellular Chloride Channel

Abstract: ERK7, a member of the mitogen-activated protein kinase family, has a carboxyl-terminal tail that is required for ERK7 activation, cellular localization, and its ability to inhibit DNA synthesis. To identify proteins that interact with ERK7, we utilized a yeast two-hybrid screen with the COOH-terminal tail of ERK7 as bait and isolated the cDNA for a novel protein termed CLIC3. The interaction between CLIC3 and ERK7 in mammalian cells was confirmed by co-immunoprecipitation. CLIC3 has significant homology to hum… Show more

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Cited by 110 publications
(101 citation statements)
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“…This latter is predominantly localised in the nucleus and stimulates New NSRD locus: DFNB33 M Medlej-Hashim et al 392 chloride conductance when expressed in cells. 15 The CLIC3 gene is a small gene composed of five exons. Screening of its genomic sequence in one patient did not reveal any sequence variant, whether a mutation or a polymorphism, which allowed exclusion of CLIC3 as a potential candidate gene.…”
Section: Resultsmentioning
confidence: 99%
“…This latter is predominantly localised in the nucleus and stimulates New NSRD locus: DFNB33 M Medlej-Hashim et al 392 chloride conductance when expressed in cells. 15 The CLIC3 gene is a small gene composed of five exons. Screening of its genomic sequence in one patient did not reveal any sequence variant, whether a mutation or a polymorphism, which allowed exclusion of CLIC3 as a potential candidate gene.…”
Section: Resultsmentioning
confidence: 99%
“…Indirect immunofluorescence, cell fractionation and immunoblotting studies have localized native and recombinant CLIC4 proteins both to the cytosol and to intracellular membranes. A similar, very unusual, dual localization is characteristic of many CLIC and p64-related proteins [18][19][20][21][22][23][24].…”
Section: Introductionmentioning
confidence: 93%
“…Human CLIC2 is a hypothetical protein encoded by the putative gene XAP121 [25]. The cDNA encoding human CLIC3, postulated like CLIC1 to be a nuclear membrane Cl − channel protein, was cloned during a yeast two-hybrid screen [20] for partners interacting with the C-terminal domain of an atypical mitogen-activated protein kinase, ERK-7. CLIC4 cDNA, which was cloned from rat brain by using reverse-transcriptasemediated PCR approaches based on the sequence of p64 [16,17], has both human [21,22] and mouse [23] homologues.…”
Section: Introductionmentioning
confidence: 99%
“…Chloride channels are involved in several crucial cell processes regulating cell volume, membrane potential, transepithelial transport, signal transduction, and acidification of organelles (36). Of special interest is the association of CLIC3 with the mitogen-activated protein kinase extracellular signal-regulated kinase 7, which represents an additional potential function for intracellular chloride channels in regulation of cell growth (28). Patch clamp studies indicate that members of this family are voltage-gated channel proteins.…”
Section: Mc3s5 Is a New Member Of A Class Of Highly Homologousmentioning
confidence: 99%