Molecular cloning and characterization of a novel adenylyl cyclase gene, HpAC1, involved in stress signaling in Hippeastrum x hybridum

An adenylyl cyclase with a phosphodiesterase domain in basal plants with a motile sperm system

“…There are three reports of the identification of AC genes in plants: PSiP in Zea mays 19, HpAC1 in Hippeastrum x hybridum 20 and AtKUP7 in Arabidopsis thaliana 21. However, the amino acid sequence of PSiP did not show any homology to known ACs.…”

Elucidation of the biochemical and functional properties of an unknown novel protein from Arabidopsis thaliana

“…This test was performed using a mutant E. coli strain (the SP850 cyaA) that is systematically deficient in endogenous AC activity and therefore cannot ferment lactose (Moutinho et al, 2001). When this mutant strain is grown on MacConkey agar, it normally produces white/yellowish colonies as compared to the magenta red/deep purple colonies produced by its wild-type counterpart (Moutinho et al, 2001, Ruzvidzo et al, 2013Swiezawska et al, 2014). Therefore and in order to test if the anticipated functional activity of the partially expressed recombinant AC-like protein could rescue this mutant strain, cells of this mutant strain were then transformed with the pTrcHis2-TOPO:AC-like gene fragment expression construct followed an assessment of the colony phenotypes on MacConkey agar medium supplemented with 0.1 mM IPTG (Figure 3.5).…”

“…The major aim and research question of this study was to gain insight into ACs and establish if there was any other additional functional AC in higher plants besides the only and already experimentally confirmed; Zea mays pollen signaling protein (Moutinho et al, 2001), Arabidopsis thaliana pentatricopeptide repeat protein (Ruzvidzo et al, 2013), Nicotiana benthamiana adenylyl cyclase protein (Ito et al, 2014), and Hippeastrum hybridum adenylyl cyclase protein (Swiezawska et al, 2014) and if so, whether such an AC was capable of mediating plant responses to different environmental signals particularly the biotic and abiotic stress factors. This study then specifically addressed the sought research question by focusing onto an investigation of the possible functional properties of a recently identified and bioinformatically annotated AC-like gene (At3g21465) in the Arabidopsis genome (Gehring, 2010).…”

“…Nicotiana benthamiana adenylyl cyclase protein (Ito et al, 2014), and Hippeastrum hybridum adenylyl cyclase protein (Swiezawska et al, 2014…”

“…Surprisingly, not much is known in higher plants about ACs or their product cAMP as is in animals and lower eukaryotes although currently, there are already four experimentally tested and functionally confirmed ACs in plants. These are the Zea mays pollen signaling protein responsible for the polarized pollen tube growth (Moutinho et al, 2001), the Arabidopsis thaliana pentatricopeptide repeat protein responsible for pathogen responses and gene expressions (Ruzvidzo et al, 2013), the Nicotiana benthamiana adenylyl cyclase protein responsible for the tabtoxinine-β-lactam-induced cell deaths during wildfire diseases (Ito et al, 2014), and the Hippeastrum hybridum adenylyl cyclase protein involved in stress signaling (Swiezawska et al, 2014).…”