Molecular cloning and characterization of Fag t 2: a 16-kDa major allergen from Tartary buckwheat seeds

Chen, P.; Guo, Yanfei; Qiu, Yan; Li, Y. H.

doi:10.1111/j.1398-9995.2011.02657.x

Cited by 16 publications

(6 citation statements)

References 8 publications

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“…SSPs are the main buckwheat allergen, and tens of their encoding sequences have been reported, mostly in common buckwheat 45 , 46 . In tartary buckwheat, three SSPs have been identified, including a 16-kDa major allergen belonged to 2S albumin 47 , a 24 kDa allergenic protein homology with a legumin-like protein 48 , and an allergen with high similarity to legume-like 13S globulin storage protein 49 . In our transcriptome, we identified 10 DEGs encoding 13S globulins (7) and 7S globulin-like proteins (3) (Fig.…”

Section: Discussionmentioning

confidence: 99%

Global transcriptome analysis and identification of genes involved in nutrients accumulation during seed development of rice tartary buckwheat (Fagopyrum Tararicum)

Huang

Deng

Shi

et al. 2017

Sci Rep

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Tartary buckwheat seeds are rich in various nutrients, such as storage proteins, starch, and flavonoids. To get a good knowledge of the transcriptome dynamics and gene regulatory mechanism during the process of seed development and nutrients accumulation, we performed a comprehensive global transcriptome analysis using rice tartary buckwheat seeds at different development stages, namely pre-filling stage, filling stage, and mature stage. 24 819 expressed genes, including 108 specifically expressed genes, and 11 676 differentially expressed genes (DEGs) were identified. qRT-PCR analysis was performed on 34 DEGs to validate the transcriptome data, and a good consistence was obtained. Based on their expression patterns, the identified DEGs were classified to eight clusters, and the enriched GO items in each cluster were analyzed. In addition, 633 DEGs related to plant hormones were identified. Furthermore, genes in the biosynthesis pathway of nutrients accumulation were analyzed, including 10, 20, and 23 DEGs corresponding to the biosynthesis of seed storage proteins, flavonoids, and starch, respectively. This is the first transcriptome analysis during seed development of tartary buckwheat. It would provide us a comprehensive understanding of the complex transcriptome dynamics during seed development and gene regulatory mechanism of nutrients accumulation.

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Section: Discussionmentioning

confidence: 99%

Global transcriptome analysis and identification of genes involved in nutrients accumulation during seed development of rice tartary buckwheat (Fagopyrum Tararicum)

Huang

Deng

Shi

et al. 2017

Sci Rep

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“…In-vitro cross-sensitivity between common buckwheat and other plant allergens has been demonstrated. Specific IgE to common buckwheat cross-reacts with tartary buckwheat (commonly grown and consumed in China),24 25 rice,26 poppy seed,27 latex,5 cashew, walnut and sesame 23. Clinical cross-reactivity with latex was noted in two latex allergic patients who had severe buckwheat reactions 5.…”

Section: Discussionmentioning

confidence: 99%

Buckwheat allergy: a potential problem in 21st century Britain

Sammut

Dennison²,

Venter³

et al. 2011

Case Reports

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“…Breeding programs include the development of adapted high-yield varieties that have other good agronomic characteristics, such as high levels of rutin, the very high biological value of the proteins, or reduced levels of antinutritional factors and zero allergenic protein [19,119,120]. In the future, it would make sense for buckwheat breeding programs to include genotypes with larger endosperm and smaller embryo as an option to reduce allergenic proteins to produce specific low allergenicity buckwheat varieties.…”

Section: Buckwheat Breeding For High Protein Levels: Prospects For Thmentioning

confidence: 99%

Breeding Buckwheat for Increased Levels and Improved Quality of Protein

Luthar

Zhou

Golob

et al. 2020

Plants

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Tartary buckwheat (Fagopyrum tataricum (L.) Gaertn.) and common buckwheat (Fagopyrum esculentum Moench) are important sources of proteins with balanced amino-acid compositions, and thus of high nutritional value. The polyphenols naturally present in Tartary buckwheat and common buckwheat lower the true digestibility of the proteins. Digestion-resistant peptides are a vehicle for fecal excretion of steroids, and in this way, for bile acid elimination and reduction of cholesterol concentrations in serum. Buckwheat proteins are more effective compared to soy proteins for the prevention of gallstone formation. Tartary and common buckwheat grain that contains appropriate amounts of selenium-containing amino acids can be produced as functional food products. The protein-rich by-products of buckwheat are a good source of bioactive substances that can suppress colon carcinogenesis by reducing cell proliferation. The grain embryo is a rich source of proteins, so breeding buckwheat with larger embryos is a possible strategy to increase protein levels in Tartary and common buckwheat grain. However, chemical analysis of the grain is the most relevant criterion for assessing grain protein levels and quality.

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Molecular cloning and characterization of Fag t 2: a 16-kDa major allergen from Tartary buckwheat seeds

Cited by 16 publications

References 8 publications

Global transcriptome analysis and identification of genes involved in nutrients accumulation during seed development of rice tartary buckwheat (Fagopyrum Tararicum)

Global transcriptome analysis and identification of genes involved in nutrients accumulation during seed development of rice tartary buckwheat (Fagopyrum Tararicum)

Buckwheat allergy: a potential problem in 21st century Britain

Breeding Buckwheat for Increased Levels and Improved Quality of Protein

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