Molecular cloning and characterization of a glucan synthase gene from the human pathogenic fungusParacoccidioides brasiliensis

Pereira, Maristela; Felipe, Maria Sueli Soares; Brígido, Marcelo M.; Soares, C. M. A.; Azevedo, M. O.

doi:10.1002/(sici)1097-0061(20000330)16:5<451::aid-yea540>3.0.co;2-o

Cited by 59 publications

(47 citation statements)

References 21 publications

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“…(132,149,218,278). A single FKS orthologue is also found in the completed genomes of Aspergillus nidulans, Aspergillus fumigatus, Neurospora crassa, and Rhizopus oryzae, indicating that these organisms also possess a single FKS gene that is likely essential (17,114,133,249).…”

Section: Fig 1 Schematic Representation Of Cell Wall Components Andmentioning

confidence: 99%

Cell Wall Assembly in Saccharomyces cerevisiae

Lesage

Bussey

2006

Microbiol Mol Biol Rev

731

749

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SUMMARY An extracellular matrix composed of a layered meshwork of β-glucans, chitin, and mannoproteins encapsulates cells of the yeast Saccharomyces cerevisiae. This organelle determines cellular morphology and plays a critical role in maintaining cell integrity during cell growth and division, under stress conditions, upon cell fusion in mating, and in the durable ascospore cell wall. Here we assess recent progress in understanding the molecular biology and biochemistry of cell wall synthesis and its remodeling in S. cerevisiae. We then review the regulatory dynamics of cell wall assembly, an area where functional genomics offers new insights into the integration of cell wall growth and morphogenesis with a polarized secretory system that is under cell cycle and cell type program controls.

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Section: Fig 1 Schematic Representation Of Cell Wall Components Andmentioning

confidence: 99%

Cell Wall Assembly in Saccharomyces cerevisiae

Lesage

Bussey

2006

Microbiol Mol Biol Rev

731

749

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“…The P. brasiliensis isolate Pb01 (ATCC, MYA 826) has been previously investigated by our group (Pereira et al, 2000;Fonseca et al, 2001). It was cultivated in Fava-Neto's medium (Fava-Neto, 1961) at 23…”

Section: Microrganism and Growth Conditionsmentioning

confidence: 99%

“…The λ Dash II genomic library, isolate Pb01, was prepared as described by Pereira et al (2000). The library (approximately 10 6 plaques) was screened by using a PCR fragment of 660 bp of the chitinase gene of Coccidioides immitis, cts2 (Pishko et al, 1995).…”

Section: Screening Of P Brasiliensis Genomic Librarymentioning

confidence: 99%

Characterization of a chaperone ClpB homologue ofParacoccidioides brasiliensis

Jesuino

Azevedo

Felipe

et al. 2002

Yeast

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“…FKS2 is required for sporulation, and a third gene in yeast, FKS3, is nonessential for growth or sporulation (41). FKS1 homologs have been reported in the pathogenic fungi C. albicans, Cryptococcus neoformans, Paracoccidioides brasiliensis, and Pneumocystis carinii and the filamentous fungi Aspergillus nidulans and Aspergillus fumigatus (3,10,28,35,41). The FKS1 genes have been demonstrated to be essential single-copy genes in C. albicans, C. neoformans, and A. fumigatus (3,10,41).…”

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confidence: 99%

Coccidioides posadasii Contains a Single 1,3-β-Glucan Synthase Gene That Appears To Be Essential for Growth

et al. 2005

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1,3-␤-Glucan synthase is responsible for the synthesis of ␤-glucan, an essential cell wall structural component in most fungi. We sought to determine whether Coccidioides posadasii possesses genes homologous to known fungal FKS genes that encode the catalytic subunit of 1,3-␤-glucan synthase. A single gene, designated FKS1, was identified, and examination of its predicted protein product showed a high degree of conservation with Fks proteins from other filamentous fungi. FKS1 is expressed at similar levels in mycelia and early spherulating cultures, and expression decreases as the spherules mature. We used Agrobacterium-mediated transformation to create strains that harbor ⌬FKS1::hygB, a null allele of FKS1, and hypothesize that Fks1p function is essential, due to our inability to purify this allele away from a complementing wild-type FKS1 allele in a heterokaryotic strain. The heterokaryon appears normal with respect to growth rate and arthroconidium production; however, microscopic examination of strains with ⌬FKS1::hygB alleles revealed abnormal swelling of hyphal elements.

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Molecular cloning and characterization of a glucan synthase gene from the human pathogenic fungusParacoccidioides brasiliensis

Cited by 59 publications

References 21 publications

Cell Wall Assembly in Saccharomyces cerevisiae

Cell Wall Assembly in Saccharomyces cerevisiae

Characterization of a chaperone ClpB homologue ofParacoccidioides brasiliensis

Coccidioides posadasii Contains a Single 1,3-β-Glucan Synthase Gene That Appears To Be Essential for Growth

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