Molecular cloning and characterization of avian N-methyl-d-aspartate receptor type1 (NMDA-R1) gene

Kurosawa, Norio; Kondo, Kazunori; Kimura, Naohiro; Ikeda, Tomio; Tsukada, Yasuzo

doi:10.1007/bf00971333

Cited by 27 publications

(25 citation statements)

References 16 publications

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“…The sequence comparison of the Apteronotus NR1 protein segment with homologous segments from rat, human, and duck. The fish sequence begins at a position that is homologous to that of amino acid 48 of the rat (Anantharam et al, 1992) and duck (Kurosawa et al, 1994) sequences and that of amino acid 53 of the human sequence (Planells-Cases et al, 1993). Boxes indicate residues that are identical in two or more sequences, and numbers indicate the amino acid positions.…”

Section: Isolation Of the A Leptorhynchus Nmdar1 Partial Cdnamentioning

confidence: 99%

N-methyl-D-aspartate receptor 1 mRNA distribution in the central nervous system of the weakly electric fishApteronotus leptorhynchus

et al. 1997

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We have isolated a partial cDNA for the N-methyl-D-aspartate (NMDA) receptor 1 (NMDAR1) subunit from an Apteronotus leptorhynchus brain cDNA library. The A. leptorhynchus cDNA fragment, which corresponds to nucleotides 135-903 within the 5' region of the rat NR1 mRNA, encodes 252 amino acids that are >80% identical to the homologous segments of the rat, human, and duck NR1 proteins. RNAse protection assays revealed that the A. leptorhynchus NR1 mRNA was highly enriched in the forebrain and hypothalamus, with lesser amounts in the brainstem, and very low levels in the cerebellum. In situ hybridization also demonstrated that neurons in the pallial forebrain were highly enriched in NR1 transcripts. High levels of NR1 mRNA were found in pyramidal cells within the optic tectum and octavolateral regions. Pyramidal cells of the electrosensory lateral line lobe had the highest levels of expression, and the NR1 mRNA was found to be selectively enriched in their apical dendrites.

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Section: Isolation Of the A Leptorhynchus Nmdar1 Partial Cdnamentioning

confidence: 99%

N-methyl-D-aspartate receptor 1 mRNA distribution in the central nervous system of the weakly electric fishApteronotus leptorhynchus

et al. 1997

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“…First, functional NMDA receptors cannot form in the absence of the NMDAR1 subunit, and physiological and ligand binding studies demonstrate the presence of functional NMDA receptors in turtles [Young et al, 1990;Larson-Prior et al, 1991;Blanton and Kriegstein, 1992]. Secondly, an NMDAR1 subunit has been demonstrated in chick [Kurosawa et al, 1994] and in a lizard species [Rodger et al, 1999], as well as in Xenopus laevis and a teleost species [Soloviev et al, 1997;Dunn et al, 1998]. Thus, it seems highly unlikely that turtles would possess NMDA receptors lacking an NR1 subunit.…”

Section: Discussionmentioning

confidence: 99%

“…Nonetheless, because functional NMDA receptors cannot form without the NMDAR1 subunit [Seeburg, 1993;Hollman and Heinemann, 1994], because functional NMDA receptors have been demonstrated in turtle nervous system by electrophysiological means [Larson-Prior et al, 1991;Blanton and Kriegstein, 1992] and because an NR1 subunit has been demonstrated in an avian species [Kurosawa et al, 1994] and in a lizard species [Rodger et al, 1999], it seems likely that NMDAR1 subunits are present in turtle brain. Our failure to detect these subunits with a monoclonal antibody directed against rat NMDAR1 seems likely to reflect the fact that the NMDAR1 epitope against which this antibody is directed is sufficiently different in turtles that the antibody against a mammalian NMDAR1 does not recognize turtle NMDAR1.…”

Section: Methodsmentioning

confidence: 99%

Immunohistochemical Localization of NMDA- and AMPA-Type Glutamate Receptor Subunits in the Basal Ganglia of Red-Eared Turtles

Fowler

Medina²,

Reiner³

1999

Brain Behav Evol

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Corticostriatal and thalamostriatal projection systems have been shown to utilize glutamate as a neurotransmitter in mammals and birds. Although corticostriatal and thalamostriatal projection systems have been demonstrated in turtles, it is uncertain whether they too use glutamate as their neurotransmitter. Immunohistochemical localization of glutamate and of NMDA- and AMPA-type ionotropic glutamate receptor subunits (NMDAR2A/B, GluR1, GluR2/3, and GluR4) were used to address this issue. Numerous medium-sized neurons that were rich in NMDAR2A/B and GluR2/3 were observed in the striatal part of the basal ganglia of red-eared turtles. Smaller numbers of medium-sized neurons and some large neurons rich in the GluR1 and GluR4 subunits were also observed in the striatum. The striatal neuropil was notably rich in GluR1, GluR2/3 and NMDAR2A/B subunits. The pallidal region was specifically rich in large neurons possessing GluR4 subunits. Consistent with the glutamate receptors on striatal and pallidal neurons, sources of input to the striatum and pallidum in turtle such as the dorsomedial and dorsolateral thalamic nuclei (which appear to correspond to intralaminar thalamic nuclei), telencephalic pallial cell groups, and the apparent subthalamic nucleus homologue were rich in glutamatergic neurons. The results show that the thalamostriatal, corticostriatal and subthalamo-pallidal projection systems of turtles are glutamatergic and that similar basal ganglia cell types in turtles and mammals have largely similar glutamate receptor characteristics.

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“…Recently biochemical studies have shown that these three receptors are pentameric complexes ) composed of selected subunits of different kinds (Brose et al, 1994) but structurally homologous: GluR1-4 for AMPA receptors (Hollmann et al, 1989;Keinanen et al, 1990), GluR5-7 and KA1-2 for kainate receptors (Egebjerg et al, 1991;Werner et al, 1991;Bettler et al, 1992;Herb et al, 1992) and NR1-2 for NMDA receptors (Moriyoshi et al, 1991;Monyer et al, 1992). The AMPA receptors (Ottiger et al, 1995) and some NMDA subunits (Kurosawa et al, 1994;Basham et al, 1999) have been (partially) cloned in birds, but, to our knowledge, kainate receptors have never been cloned nor purified in any avian species. A kainate-binding protein (KBP) with high sequence homology to the mammalian kainate receptor has however been cloned in chicken.…”

mentioning

confidence: 98%

“…In birds, a few studies have characterized the binding and/or sequence of several types of glutamate receptors (Bowie and Smart, 1994;Kurosawa et al, 1994;Ottiger et al, 1995;Lowe et al, 1997;Jacobsson et al, 1998;Basham et al, 1999). The distribution of the receptor subunits GluR1-4 has been studied in the pigeon by in situ hybridization (Ottiger et al, 1995) and by immunocytochemistry (Ottiger et al, 1995).…”

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confidence: 99%

Immunocytochemical localization of ionotropic glutamate receptors subunits in the adult quail forebrain

et al. 2000

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The excitatory amino acid glutamate is implicated in the central control of many neuroendocrine and behavioral processes. The ionotropic glutamate receptors are usually divided into the N-methyl-D-aspartate (NMDA) and non-NMDA (kainate and AMPA) subtypes. Subunits of these receptors have been cloned in a few mammalian species. Information available in birds is more limited. In quail, we recently demonstrated that glutamate agonists (kainate, AMPA, and NMDA) rapidly (within minutes) and reversibly decrease in vitro aromatase activity like several other manipulations affecting intracellular HCa(2+) pools. Aromatase catalyzes the conversion of androgens into estrogens which is a limiting step in the control by testosterone of many behavioral and physiologic processes. Therefore, glutamate could control estrogen production in the brain, but the anatomic substrate supporting this effect is poorly understood. In quail, aromatase is mainly localized in the preoptic-hypothalamic-limbic system. We visualized here the distribution of the major ionotropic glutamate receptors in quail by immunocytochemical methods by using commercial primary antibodies raised against rat glutamate receptor 1 and receptors 2-3 (GluR1, GluR2/3: AMPA subtype, Chemicon, CA), rat glutamate receptors 5-7 (GluR5-7: kainate subtype, Pharmingen, CA), and rat NMDA receptors (NMDAR1, Pharmingen, CA). Dense and specific signals were obtained with all antibodies. The four types of receptors are broadly distributed in the brain, and, in particular, immunoreactive cells are identified within the major aromatase cell groups located in the medial preoptic nucleus, ventromedial hypothalamus, nucleus striae terminalis, and nucleus taeniae. Dense specific populations of glutamate receptor-immunoreactive cells are also present with a receptor subtype-specific distribution in broad areas of the telencephalon. The distribution of glutamate receptors, therefore, is consistent with the idea that these receptors could be located at the surface of aromatase-containing cells and mediate the rapid regulation of aromatase activity in a direct manner.

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Molecular cloning and characterization of avian N-methyl-d-aspartate receptor type1 (NMDA-R1) gene

Cited by 27 publications

References 16 publications

N-methyl-D-aspartate receptor 1 mRNA distribution in the central nervous system of the weakly electric fishApteronotus leptorhynchus

N-methyl-D-aspartate receptor 1 mRNA distribution in the central nervous system of the weakly electric fishApteronotus leptorhynchus

Immunohistochemical Localization of NMDA- and AMPA-Type Glutamate Receptor Subunits in the Basal Ganglia of Red-Eared Turtles

Immunocytochemical localization of ionotropic glutamate receptors subunits in the adult quail forebrain

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