Molecular cloning and characterization of a metal responsive Chironomus tentans alpha-tubulin cDNA1Note: GeneBank Data: The nucleotide sequence reported in this paper has been deposited in the GeneBank™ with accession number AF272829.1

Mattingly, K.S.; Beaty, Barry J.; Mackie, Ryan S.; McGaw, M.; Carlson, Jonathan O.; Rayms-Keller, Alfredo

doi:10.1016/s0166-445x(00)00181-8

Cited by 17 publications

(3 citation statements)

References 26 publications

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“…Alpha-tubulin proteins are a component of the microtubules, which were considered to be associated with the heavy metal tolerance in insects [ 56 ]. Recent report indicated that the transcript level of a-tubulin in heavy metal-tolerant Anopheles mosquitoes was significantly higher under cadmium treatment than normal condition [ 57 ].…”

Section: Resultsmentioning

confidence: 99%

Transcriptome profilings of two tall fescue (Festuca arundinacea) cultivars in response to lead (Pb) stress

Amombo

et al. 2017

BMC Genomics

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BackgroundLead (Pb) is one of the most toxic heavy metal environmental pollutants. Tall fescue is an important cold season turf grass which can tolerate and accumulate substantial amount of Pb. To estimate genes related to Pb response and the molecular mechanism associated with Pb tolerance and accumulation, we analyzed the transcriptome of tall fescue in response to Pb treatment.ResultsRNA-sequencing was performed in two tall fescue cultivars, Pb tolerant Silverado and Pb sensitive AST7001. A total of 810,146 assembled unique transcripts representing 25,415 unigenes were obtained from the tall fescue leaves. Among the panel, 3,696 differentially expressed genes (DEGs) were detected between the Pb treated (1000 mg/L) and untreated samples. Gene ontology (GO) and pathway enrichment analysis demonstrated that the DEGs were mainly implicated in energy metabolism, metabolism of terpenoids and polyketides, and carbohydrate metabolism related pathways. The expression patterns of 16 randomly selected genes were in consistent with that from the Solexa analysis using quantitative reverse-transcription PCR. In addition, compared to the common transcriptional response to Pb stress in both cultivars, the regulation of numerous genes including those involved in zeatin biosynthesis, limonene and pinene degradation, phagosome was exclusive to one cultivar.ConclusionsThe tall fescue assembled transcriptome provided substantial molecular resources for further genomics analysis of turfgrass in response to heavy metal stress. The significant expression difference of specific unigenes may account for Pb tolerance or accumulation in two different tall fescue cultivars. This study provided new insights for the investigation of the molecular basis of Pb tolerance and accumulation in tall fescue as well as other related turf grass species.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-3479-3) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Transcriptome profilings of two tall fescue (Festuca arundinacea) cultivars in response to lead (Pb) stress

Amombo

et al. 2017

BMC Genomics

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“…At the molecular level, the development of tolerance to metal pollutants involves protein induction shifts [ 10 ]. For example, the stress response genes coding glutathione S-transferase [ 19 ], metallothionein [ 20 ] as well as mucin and α-tubulin have been associated with metal tolerance [ 17 , 21 , 22 ]. Several cytochrome P450 genes, particularly in the CYP6 class, have also been associated with metal tolerance.…”

Section: Introductionmentioning

confidence: 99%

The effect of metal pollution on the life history and insecticide resistance phenotype of the major malaria vector Anopheles arabiensis (Diptera: Culicidae)

Oliver

Brooke

2018

PLoS ONE

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Metal exposure is one of the commonest anthropogenic pollutants mosquito larvae are exposed to, both in agricultural and urban settings. As members of the Anopheles gambiae complex, which contains several major malaria vector species including An. arabiensis, are increasingly adapting to polluted environments, this study examined the effects of larval metal exposure on various life history traits of epidemiological importance. Two laboratory strains of An. arabiensis, SENN (insecticide susceptible) and SENN DDT (insecticide resistant), were reared in maximum acceptable toxicity concentrations, (MATC—the highest legally accepted concentration) of cadmium chloride, lead nitrate and copper nitrate. Following these exposures, time to pupation, adult size and longevity were determined. Larvae reared in double the MATC were assessed for changes in malathion and deltamethrin tolerance, measured by lethal time bottle bioassay, as well as changes in detoxification enzyme activity. As defence against oxidative stress has previously been demonstrated to affect the expression of insecticide resistance, catalase, glutathione peroxidase and superoxide dismutase activity was assessed. The relative metal toxicity to metal naïve larvae was also assessed. SENN DDT larvae were more tolerant of metal pollution than SENN larvae. Pupation in SENN larvae was significantly reduced by metal exposure, while adult longevity was not affected. SENN DDT showed decreased adult size after larval metal exposure. Adult insecticide tolerance was increased after larval metal exposure, and this effect appeared to be mediated by increased β-esterase, cytochrome P450 and superoxide dismutase activity. These data suggest an enzyme-mediated positive link between tolerance to metal pollutants and insecticide resistance in adult mosquitoes. Furthermore, exposure of larvae to metal pollutants may have operational consequences under an insecticide-based vector control scenario by increasing the expression of insecticide resistance in adults.

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“…Cadmium and Pb tolerant A. gambiae mosquitoes have significant reduction in expression of the CYP6 family of cytochrome p450 genes, a gene family associated with resistance to pyrethroid pesticides ( Musasia et al, 2013 ), suggesting potential interactions between metal tolerance and pesticide sensitivity. Expression of the alpha-tubulin gene also responds to metal concentrations in both A. gambiae ( Mireji et al, 2010a ) and Chironomus tentans midges ( Mattingly et al, 2001 ). This is not an exhaustive list, and as more efforts are put in to broad-based exploratory studies (see below) it will continue to grow.…”

Section: Classical Genetic Responsesmentioning

confidence: 99%

Genetic Diversity in Insect Metal Tolerance

Merritt

Bewick

2017

Front. Genet.

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Insects encounter a variety of metals in their environment, many of which are required at some concentration for normal organismal homeostasis, but essentially all of which are toxic at higher concentrations. Insects have evolved a variety of genetic, and likely epigenetic, mechanisms to deal with metal stress. A recurring theme in all these systems is complexity and diversity; even simple, single gene, cases are complex. Of the known gene families, the metallothioneins are perhaps the best understood and provide good examples of how diverse metal response is. Interestingly, there is considerable diversity across taxa in these metal-responsive systems, including duplications to form small gene families and complex expression of single loci. Strikingly, different species have evolved different mechanisms to cope with the same, or similar, stress suggesting both independent derivation of, and plasticity in, the pathways involved. It is likely that some metal-response systems evolved early in evolutionary time and have been conserved, while others have diverged, and still others evolved more recently and convergently. In addition to conventional genetics, insects likely respond to environmental metal through a variety of epigenetic systems, but direct tests are lacking. Ultimately, it is likely that classical genetic and epigenetic factors interact in regulating insect metal responses. In light of this diversity across species, future studies including a broad-based examination of gene expression in non-model species in complex environments will likely uncover additional genes and genetic and epigenetic mechanisms.

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Molecular cloning and characterization of a metal responsive Chironomus tentans alpha-tubulin cDNA1Note: GeneBank Data: The nucleotide sequence reported in this paper has been deposited in the GeneBank™ with accession number AF272829.1

Cited by 17 publications

References 26 publications

Transcriptome profilings of two tall fescue (Festuca arundinacea) cultivars in response to lead (Pb) stress

Transcriptome profilings of two tall fescue (Festuca arundinacea) cultivars in response to lead (Pb) stress

The effect of metal pollution on the life history and insecticide resistance phenotype of the major malaria vector Anopheles arabiensis (Diptera: Culicidae)

Genetic Diversity in Insect Metal Tolerance

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