Molecular cloning and characterization of phenylalanine ammonia-lyase, cinnamate 4-hydroxylase and genes involved in flavone biosynthesis in Scutellaria baicalensis

Xu, Hui; Park, Nam Il; Li, Xiaohua; Kim, Yong Kyoung; Lee, Sook Young; Park, Sang Un

doi:10.1016/j.biortech.2010.07.083

Cited by 90 publications

(75 citation statements)

References 34 publications

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“…In this study, PAL, C4H, and 4CLs were first cloned and characterized from P. vulgaris. Recently, PAL cDNA has been isolated and characterized from S. miltiorrhiza, 21) M. officinalis, 26) A. rugosa, 27) S. baicalensis, 28) and Allium sativum. 29) Our group previously reported transcription of genes asso- ciated with tilianin and RA biosynthetic pathways (PAL, C4H, 4CL, CHS, and CHI) in A. rugosa.…”

Section: Discussionmentioning

confidence: 99%

Molecular Cloning and Characterization of Genes Involved in Rosmarinic Acid Biosynthesis from Prunella vulgaris

Kim

Shin

Tuấn

et al. 2014

Biological & Pharmaceutical Bulletin

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Prunella vulgaris L., commonly known as "self-heal" or "heal-all," is a perennial herb with a long history of medicinal use. Phenylalanine ammonia-lyase (PAL), cinnamate 4-hydroxylase (C4H), and 4-coumarate:coenzyme-A (CoA) ligase (4CL) are important enzymes in the phenylpropanoid pathway and in the accumulation of rosmarinic acid (RA), which is a major secondary metabolite in P. vulgaris. In this study, we isolated cDNAs encoding PvPAL, PvC4H, and Pv4CL from P. vulgaris using rapid amplification of cDNA ends polymerase chain reaction (PCR). The amino acid sequence alignments of PvPAL, PvC4H, and Pv4CL showed high sequence identity to those of other plants. Quantitative real-time PCR analysis was used to determine the transcript levels of genes involved in RA biosynthesis in the flowers, leaves, stems, and roots of P. vulgaris. The transcript levels of PvPAL, PvC4H, and Pv4CL1 were the highest in flowers, whereas Pv4CL2 was the highest in roots. High-performance liquid chromatography analysis also showed the highest RA content in the flowers (3.71 mg/g dry weight). We suggest that the expression of the PvPAL, PvC4H, and Pv4CL1 genes is correlated with the accumulation of RA. Our results revealed that P. vulgaris flowers are appropriate for medicinal usage, and our findings provide support for increasing RA production in this plant.

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Section: Discussionmentioning

confidence: 99%

Molecular Cloning and Characterization of Genes Involved in Rosmarinic Acid Biosynthesis from Prunella vulgaris

Kim

Shin

Tuấn

et al. 2014

Biological & Pharmaceutical Bulletin

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“…Unlike SbPAL2 and SbPAL3, however, SbPAL1 localization was entirely cytosolic. This result may be related that SbPAL1 formed a distinct group with SbPAL2 and SbPAL3 in a phylogenetic tree constructed from the deduced amino acid sequences of plant PALs (Xu et al, 2010).…”

Section: Discussionmentioning

confidence: 70%

Subcellular Localization Studies of Three Phenylalanine Ammonia-Lyases and Cinnamate 4-Hydroxylase from Scutellaria Baicalensis Using GFP Fusion Proteins

Park¹,

Xu²,

Arasu³

et al. 2015

OnLine Journal of Biological Sciences

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The localization of three phenylalanine ammonia-lyases (PAL1, -2 and -3) and Cinnamate 4-Hydroxlase (C4H) of Scutellaria baicalensis was examined in onion epidermal cells. These genes encode key enzymes in the phenylpropanoid pathway for the synthesis of flavones. In our previous research, we isolated coding DNA for these genes from S. baicalensis, a medicinal herb rich in flavones with biological and pharmacological properties. We observed that SbPAL2, SbPAL3 and SbC4H proteins localize to the endoplasmic reticulum; however, SbPAL1 was a cytosolic protein. Unlike SbPAL2 and SbPAL3, SbPAL1 may be expected to have a different function in the flavone biosynthetic pathway.

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“…In the final step, the resulting (2S)-pinocembrin chalcone is stereospecifically isomerized by chalcone isomerase (CHI) to form (2S)-pinocembrin ( Fig. 1) (Leonard et al, 2008;Putignani et al, 2013;Xu et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Stepwise modular pathway engineering of Escherichia coli for efficient one-step production of (2S)-pinocembrin

Zhang

Dong

et al. 2016

Journal of Biotechnology

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Molecular cloning and characterization of phenylalanine ammonia-lyase, cinnamate 4-hydroxylase and genes involved in flavone biosynthesis in Scutellaria baicalensis

Cited by 90 publications

References 34 publications

Molecular Cloning and Characterization of Genes Involved in Rosmarinic Acid Biosynthesis from <i>Prunella vulgaris</i>

Molecular Cloning and Characterization of Genes Involved in Rosmarinic Acid Biosynthesis from <i>Prunella vulgaris</i>

Subcellular Localization Studies of Three Phenylalanine Ammonia-Lyases and Cinnamate 4-Hydroxylase from <i>Scutellaria Baicalensis</i> Using GFP Fusion Proteins

Stepwise modular pathway engineering of Escherichia coli for efficient one-step production of (2S)-pinocembrin

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