Molecular cloning and characterization of a novel microsomal oleate desaturase gene from soybean

Li, Lingyong; Wang, Xiaolin; Gai, Junyi; Yu, Deyue

doi:10.1016/j.jplph.2006.08.007

Cited by 78 publications

(69 citation statements)

References 26 publications

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“…3) in agreement with the previous reports (Heppard et al 1996, Martínez-Rivas et al 2001, Li et al 2006. A higher expression pattern of DiFAD2 in expanding tissue suggests a correlation between the level of membrane desaturation and cell elongation (Martínez-Rivas et al 2001).…”

Section: Discussionsupporting

confidence: 89%

“…These results showed that the altered PUFA content in leaves of D. involucrata at low temperature have no direct correlation with the expression of FAD2 but there may be two or more FAD2 genes in the D. involucrata genome. Another mechanism may be conversion of 18:2 to 18:3 by δ 15 -FAD (Li et al 2006). Furthermore, D. involucrate have more highly unsaturated fatty acids (22:5 and 22:6) in leaves, which is rare in other higher plants.…”

Section: Discussionmentioning

confidence: 99%

“…They are major structural components of membrane lipids, provide a substantial reserve of free energy and serve as key precursors for the biosynthesis of messengers in signal transduction mechanisms that influence plant growth, development and responses to various environmental factors (Pirtle et al 2001, Wei et al 2004, Li et al 2006. Fatty acid desaturases (FADs) are enzymes responsible for the insertion of double bonds into fatty acyl chains, following the removal of two hydrogen atoms.…”

Section: Introductionmentioning

confidence: 99%

“…A knowledge of the tissue-specific and development-specific regulation of fatty acid compositions of the membranes is crucial in understanding the mechanisms underlying heat and cold tolerance, desiccation, salt tolerance, and disease resistance in higher plants (Heppard et al 1996, Pirtle 2001, Nakamura et al 2004. With better understanding of fatty acid metabolic pathway, many desaturase genes were cloned from plants such as Arabidopsis, Helianthus annuus, Zea mays, Glycine max and Sapium sebiferum (Okuley et al 1994, Martínez-Rivas et al 2001, Tao et al 2006, Li et al 2006, Niu et al 2007). However, little information is available on the regulation of fatty acid biosynthesis of Davidia involucrate Baill., though its seeds have a high oil content (48 %; m/m).…”

Section: Introductionmentioning

confidence: 99%

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Molecular cloning and characterization of a novel microsomal oleate desaturase gene DiFAD2 from Davidia involucrata Baill

Lei

Peng

Niu³

et al. 2010

Biologia plant.

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In the conversion of oleic acid to linoleic acid, δ 12 -fatty acid desaturase (δ 12 -FAD) is involved. Based on the conserved oligo amino acid residues of the FAD2 genes from other plants, a new full-length cDNA (DiFAD2) encoding a δ 12 -FAD was cloned from Davidia involucrata Baill. Sequence analysis indicated that the DiFAD2 gene had an open reading frame (ORF) of 1 149 bp, coding for 382 amino acids residues of 44.3 kDa, pI of the deduced protein was 8.8. The deduced amino acid sequence of the cloned DiFAD2 showed high identities to those genes of other plant δ 12 -FAD. RT-PCR showed that DiFAD2 was expressed in all tissues and expression was abundant in young stems. Expression of DiFAD2 is not enhanced by low temperature and the altered polyunsaturated fatty acid content in leaves treated with low temperature may be due to the post-transcriptional regulation of the DiFAD2 gene or the other FAD2 gene family regulation.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Molecular cloning and characterization of a novel microsomal oleate desaturase gene DiFAD2 from Davidia involucrata Baill

Lei

Peng

Niu³

et al. 2010

Biologia plant.

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“…plant microsomal desaturases, such as fad2 genes from cotton (Pirtle et al 2001;Zhang et al 2005), tung (Dyer et al 2002), olive (Hernandez et al 2005), and soybean (Li et al 2007), have been reported previously and their functional identity was established in S. cerevisiae.…”

Section: Resultsmentioning

confidence: 96%

Functional expression of Brassica juncea oleate desaturase gene (Bjfad2) in Escherichia coli

2013

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The synthesis of polyunsaturated fatty acids, the most abundant fatty acids in plants, begins with a reaction catalyzed by fatty acid desaturase-2 (FAD2; EC 1.3.1.35), also called as microsomal ∆12 oleate desaturase. The gene (Bjfad2; GenBank accession No. EF639848) coding for this enzyme from Brassica juncea was previously isolated and characterized. However, functional identity of Bjfad2 was not established. Utilizing the known Bjfad2 cDNA sequence, the ORF of Bjfad2 gene was cloned into the pMAL C2X Escherichia coli expression vector and produced recombinant plasmid by insertion of isolated ORF downstream to the maltose-binding protein coding sequence. The pMALC2X-Bjfad2 vector was used to transform the TB1 strain of E. coli. Induced expression of pMAL-BJFAD2 fused product resulted in the synthesis of a polypeptide with an apparent molecular mass of 80 kDa, which was 8 kDa less than calculated mass as determined by SDS-PAGE, since the fused MalE-Bjfad2 gene contains eight additional codons located between the MalE and Bjfad2 gene. In vitro activity assay of oleate desaturase using the corresponding bacterial crude extracts confirmed that the polypeptide was the product of the Bjfad2 gene. The reaction products analysis of the fatty acid methyl esters by gas chromatography showed the presence of a new peak with a similar retention time to linoleic acid, which was absent in the control activity assay without electron donors. Thus, B. juncea gene has been functionally identified since it encodes the enzyme that catalyzed the desaturation of oleate to linoleate.

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XsFAD2 gene encodes the enzyme responsible for the high linoleic acid content in oil accumulated in Xanthoceras sorbifolia seeds

Guo

Wang

et al. 2013

J. Sci. Food Agric.

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Molecular cloning and characterization of a novel microsomal oleate desaturase gene from soybean

Cited by 78 publications

References 26 publications

Molecular cloning and characterization of a novel microsomal oleate desaturase gene DiFAD2 from Davidia involucrata Baill

Molecular cloning and characterization of a novel microsomal oleate desaturase gene DiFAD2 from Davidia involucrata Baill

Functional expression of Brassica juncea oleate desaturase gene (Bjfad2) in Escherichia coli

XsFAD2 gene encodes the enzyme responsible for the high linoleic acid content in oil accumulated in Xanthoceras sorbifolia seeds

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