Molecular cloning and characterization of a glycosyl hydrolase family 9 cellulase distributed throughout the digestive tract of the cricket Teleogryllus emma

Kim, Namjung; Choo, Young Moo; Lee, Kwang-Sik; Hong, Seong Jin; Seol, Kwang Youl; Je, Yeon Ho; Sohn, Hung Dae; Jin, Byung Rae

doi:10.1016/j.cbpb.2008.04.005

Cited by 58 publications

(61 citation statements)

References 26 publications

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“…1). Protein alignment followed by phylogenetic tree analysis revealed that NlEG1 is homologous to insect endob-1,4-glucanases and belongs to the glycosyl hydrolase family 9 (GHF 9); this family is characterized by catalytic domains, including a catalytic nucleophile (Asp-77), a probable secondary nucleophile (Asp-80), and a proton acceptor (Glu-435), and two signature motifs (Nakashima et al, 2002;Kim et al, 2008;Willis et al, 2011;Fig. 1;Supplemental Figs.…”

Section: Isolation and Characterization Of Nleg1mentioning

confidence: 99%

A Salivary Endo-β-1,4-Glucanase Acts as an Effector That Enables the Brown Planthopper to Feed on Rice

Chen

et al. 2017

Plant Physiol.

100

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ORCID IDs: 0000-0002-5714-7586 (J.Z.); 0000-0002-3262-6134 (Y.L.).The brown planthopper (BPH) Nilaparvata lugens is one of the most destructive insect pests on rice (Oryza sativa) in Asia. After landing on plants, BPH rapidly accesses plant phloem and sucks the phloem sap through unknown mechanisms. We discovered a salivary endo-b-1,4-glucanase (NlEG1) that has endoglucanase activity with a maximal activity at pH 6 at 37°C and is secreted into rice plants by BPH. NlEG1 is highly expressed in the salivary glands and midgut. Silencing NlEG1 decreases the capacity of BPH to reach the phloem and reduces its food intake, mass, survival, and fecundity on rice plants. By contrast, NlEG1 silencing had only a small effect on the survival rate of BPH raised on artificial diet. Moreover, NlEG1 secreted by BPH did not elicit the production of the defense-related signal molecules salicylic acid, jasmonic acid, and jasmonoyl-isoleucine in rice, although wounding plus the application of the recombination protein NlEG1 did slightly enhance the levels of jasmonic acid and jasmonoyl-isoleucine in plants compared with the corresponding controls. These data suggest that NlEG1 enables the BPH's stylet to reach the phloem by degrading celluloses in plant cell walls, thereby functioning as an effector that overcomes the plant cell wall defense in rice.

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Section: Isolation and Characterization Of Nleg1mentioning

confidence: 99%

A Salivary Endo-β-1,4-Glucanase Acts as an Effector That Enables the Brown Planthopper to Feed on Rice

Chen

et al. 2017

Plant Physiol.

100

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“…Cellulose and hemicelluloses are the most abundant biomasses on the earth and have the greatest potential to resolve both the energetic and environmental demands of bioenergy [1,2]. Cellulose is a linear polysaccharide which consists of 1000-1200 glucose residues with β-1,4-glycosidic linkages [3].…”

Section: Introductionmentioning

confidence: 99%

Isolation, Screening and Characterization of Cellulase Producing Bacterial Isolates from Municipal Solid Wastes and Rice Straw Wastes

Khatiwada¹,

Ahmed²,

Sohag³

et al. 2016

J Bioprocess Biotech

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“…The advancement of genomics and proteomics research tools are expected to allow new insights into the mechanisms for wood deconstruction by cellulose-feeding insects, as well as facilitate the discovery of new cellulolytic enzymes from a wide range of cellulolytic system (Sun and Scharf, 2010). There have been numerous reports on cellulolytic activity in insects including identification and cloning of insect cellulases (Lee et al, 2004(Lee et al, , 2005Wei et al, 2006;Kim et al, 2008). Lee et al (2004) cloned a cellulase [beta-1,4-endoglucanase (EGase), EC 3.2.1.4] cDNA (Ag-EGase I) belonging to glycoside hydrolase family (GHF) 45 from the mulberry longicorn beetle, Apriona germari and also reported the gene structure, expression and enzyme activity of Ag-EGase I and an additional cellulase (Ag-EGase II).…”

Section: Role Of Cellulasesmentioning

confidence: 99%

“…Besides this, Wei et al (2006) cloned a novel endogenous beta-1,4-endoglucanase (Ag-EGase III) gene belonging to the glycoside hydrolase family (GHF) from A. germari. Further, Kim et al (2008) cloned and characterized a novel endogenous beta-1,4 -endoglucanase (EG) gene belonging to the glycosyl hydrolase family 9 (GHF 9) that was distributed throughout the digestive tract of the cricket, Teleogryllus emma, suggesting a functional role of endogenous TeEG-I in a sequential cellulose digestion process. The comprehensive proteome analysis of digestome of lower termite Coptotermes gestroi revealed the complete collection of hydrolytic enzymes including cellulases, hemicellulases and pectinases (Franco Cairo et al, 2011).…”

Section: Role Of Cellulasesmentioning

confidence: 99%

Insect cellulolytic enzymes: Novel sources for degradation of lignocellulosic biomass

Taggar

2015

JANS

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Alternative and renewable fuels derived from lignocellulosic biomass offer the potential to reduce our d ependence on fossil fuels and mitigate global climate change. Cellulose is one of the major structural components in all lignocellulosic wastes and enzymatic depolymerization of cellulose by cellulases is an essential step in bio-ethanol production. Wood-degrading insects are potential source of biochemical catalysts for converting wood lignocellulose into biofuels. Cellulose digestion has been demonstrated in more than 20 insect families representing ten distinct insect orders. Termite guts been have considered as the "world's smallest bioreactors" since they digest a significant proportion of cellulose (74-99%) and hemicellulose (65-87%) components of lignocelluloses they ingest. The lower termites harbor protistan symbionts in hindgut whereas higher termites lack these in the hind gut. Studies on cellulose digestion in termites and other insects with reference to ligno-cellulose degrading enzymes have been well focused in this review. The studies on insect cellulolytic systems can lead to the discovery of a variety of novel biocatalysts and genes that encode them, as well as associated unique mechanisms for efficient biomass conversion into biofuels.

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Molecular cloning and characterization of a glycosyl hydrolase family 9 cellulase distributed throughout the digestive tract of the cricket Teleogryllus emma

Cited by 58 publications

References 26 publications

A Salivary Endo-β-1,4-Glucanase Acts as an Effector That Enables the Brown Planthopper to Feed on Rice

A Salivary Endo-β-1,4-Glucanase Acts as an Effector That Enables the Brown Planthopper to Feed on Rice

Isolation, Screening and Characterization of Cellulase Producing Bacterial Isolates from Municipal Solid Wastes and Rice Straw Wastes

Insect cellulolytic enzymes: Novel sources for degradation of lignocellulosic biomass

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