Molecular Cloning And Characterization OfEthylene Overproducer 1-Like 1Gene,Leeol1, From Tomato (Lycopersicon EsculentumMill.) Fruit

Abstract

Recently, ETHYLENE OVERPRODUCER 1 (ETO1) had been cloned and identified as a negative post-transcriptional regulator in the ethylene biosynthesis in Arabidopsis. However, little was known about the role of ETO1 in other species, especially in tomato, which was an ideal model for studying the biosynthesis of ethylene during tomato fruit ripening. In this study, a tomato ETHYLENE OVERPRODUCER 1-LIKE 1 (LeEOL1) was cloned. The LeEOL1 cDNA was 3,515 bp long and carried an ORF that putatively encoded a polypeptide of 886 amino acids with a predicted molecular mass of 95 kDa. It shared 74% identity in amino acid sequence with Arabidopsis EOL1 and had one BTB (Broad-complex, Tramtrack, Bric-à-brac) domain and two TPR (tetratricopeptide repeat) domains, which were also conserved domains in AtEOL1. RT-PCR analysis of the temporal expression of LeEOL1 showed that its transcript decreased companied with increase of ethylene production in tomato ripening. The level of LeEOL1 transcripts in wild type tomato fruit at mature green stage did not distinctively change when treated with exogenous ethylene.

The WOX13 homeobox gene promotes replum formation in the Arabidopsis thaliana fruit

“…Antagonistic activities of transcription factors give rise to this medio‐lateral pattern of the Arabidopsis fruit. The FRUITFULL ( FUL , Gu et al ., ) MADS‐box gene is responsible for the development of the valves (Liljegren et al ., ), the SHATERPROOF ( SHP ) MADS‐box genes are required for the formation of the valve margins (Liljegren et al ., ), and REPLUMLESS homeobox gene ( RPL, also known as PENNYWISE; BELLRINGER, and VAAMANA , Byrne et al ., ; Bao et al ., ; Bhatt et al ., ; Smith and Hake, ; Zhu et al ., ) and the class I KNOX gene BREVIPEDICELLUS ( BP , Douglas et al ., ; Venglat et al ., ), also known as KNAT1 , Lincoln et al ., ; Chuck et al ., ) are key players in promoting replum identity (Roeder et al ., ; Alonso‐Cantabrana et al ., ; Ripoll et al ., ). The combined activities of the SHATTERPROOF ( SHP1 , SHP2 ) MADS‐box genes and the bHLH genes INDEHISCENT ( IND , Liljegren et al ., ) and ALCATRAZ ( ALC, Rajani and Sundaresan, ), and recently SPATULA ( SPT , Groszmann et al ., ; Arnaud et al ., ) have been proven to be essential for valve margin formation and thus, proper fruit dehiscence.…”