Molecular cloning and characterization of a novel gene encoding zinc finger protein from Medicago sativa L.

Chao, Yuehui; Sun, Yan; Yang, Qingchuan; Wang, Pingqing; Wu, Mingqiu; Li, Yan; Long, Ruicai; Qin, Z. H.

doi:10.1007/s11033-009-9450-5

Cited by 25 publications

(13 citation statements)

References 23 publications

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“…AB158612), respectively, were used as controls to normalize the amount of cDNA. The relative expression was calculated using the comparative ∆∆ threshold cycle method (Chao et al, 2009). All results are presented as the means of at least three independent RNA extractions (including three technical replicates) with corresponding standard deviations (SD).…”

Section: Quantitative Real-time Pcrmentioning

confidence: 99%

Molecular cloning and characterization of a chlorophyll degradation regulatory gene (ZjSGR) from Zoysia japonica

Teng¹,

Chang²,

Xiao³

et al. 2016

Genet. Mol. Res.

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Section: Quantitative Real-time Pcrmentioning

confidence: 99%

Molecular cloning and characterization of a chlorophyll degradation regulatory gene (ZjSGR) from Zoysia japonica

Teng¹,

Chang²,

Xiao³

et al. 2016

Genet. Mol. Res.

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“…The endogenous gene AsActin (GenBank: JX644005.1) was used in the comparative C T (2 -∆CT ) method, in which ∆G T = C T(target gene) -C T(reference gene) , allowing the relative quantification of gene expression. This equation was improved through the comparative ∆∆C T method (Chao et al, 2009). Four independent biological repeats were conducted for each treatment to ensure data accuracy.…”

Section: Analysis Of Asaco Gene Inductionmentioning

confidence: 99%

Cloning and expression of the 1-aminocyclopropane-1-carboxylic oxidase gene from Agrostis stolonifera

Xiao¹,

Li²,

Teng³

et al. 2016

Genet. Mol. Res.

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ABSTRACT.A gene encoding 1-aminocyclopropane-1-carboxylic oxidase (ACO), which catalyzes the terminal step in ethylene biosynthesis, was isolated from Agrostis stolonifera. The AsACO gene is composed of 975 bp, encoding 324 amino acids. Three exons interspersed by two introns form AsACO gDNA. A BLAST search of the nucleotide sequence revealed a high level of similarity (79-91%) between AsACO and ACO genes of other plants. A phylogenetic tree was constructed via BLAST in the NCBI, and revealed the highest homology with wheat TaACO. The calculated molecular mass and predicted isoelectric point of AsACO were 36.25 and 4.89 kDa, respectively. Analysis of subcellular localization revealed that AsACO is located in the nucleus and cytoplasm. The Fe(II)-binding cofactors and cosubstrate were identified, pertaining to the ACO family. The expression patterns of AsACO were determined by quantitative real time PCR. AsACO expression was highest in the stem, and was strongly up-regulated in response to ethephon, methyl jasmonate, salicylic acid, and cold temperature, but down-regulated in response to drought and NaCl treatment. The protein encoded by AsACO exhibited ACC oxidase activity in vitro. Taken together, these findings suggest that AsACO contains domains common to the ACO family, and is induced in response to exogenous hormones. Conversely, some abiotic stress conditions can inhibit AsACO expression.

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“…The alfalfa Ubiquitin gene was used as a control in order to normalize the amount of cDNA. Relative expression was calculated using the comparative DD threshold cycle method (Chao et al, 2009). All of the results are reported as means of at least three independent RNA extractions (including three technical replicates) with their corresponding standard deviations (SD).…”

Section: Quantitative Real-time Pcrmentioning

confidence: 99%

Expression of an alfalfa (Medicago sativa L.) peroxidase gene in transgenic Arabidopsis thaliana enhances resistance to NaCl and H2O2

Teng¹,

Xiao²,

Guo³

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Peroxidases (PODs) are enzymes that play important roles in catalyzing the reduction of H 2 O 2 and the oxidation of various substrates. They function in many different and important biological processes, such as defense mechanisms, immune responses, and pathogeny. The POD genes have been cloned and identified in many plants, but their function in alfalfa (Medicago sativa L.) is not known, to date. Based on the POD gene sequence (GenBank accession No. L36157.1), we cloned the POD gene in alfalfa, which was named MsPOD. MsPOD expression increased with increasing H 2 O 2 . The gene was expressed in all of the tissues, including the roots, stems, leaves, and flowers, particularly in stems and leaves under light/dark conditions. A subcellular analysis showed that MsPOD was localized outside the cells. Transgenic Arabidopsis with MsPOD exhibited increased resistance to H 2 O 2 and NaCl. Moreover, POD activity in the transgenic plants was significantly higher than that in wild-type Arabidopsis. These results show that MsPOD plays an important role in resistance to H 2 O 2 and NaCl.

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Molecular cloning and characterization of a novel gene encoding zinc finger protein from Medicago sativa L.

Cited by 25 publications

References 23 publications

Molecular cloning and characterization of a chlorophyll degradation regulatory gene (ZjSGR) from Zoysia japonica

Molecular cloning and characterization of a chlorophyll degradation regulatory gene (ZjSGR) from Zoysia japonica

Cloning and expression of the 1-aminocyclopropane-1-carboxylic oxidase gene from Agrostis stolonifera

Expression of an alfalfa (Medicago sativa L.) peroxidase gene in transgenic Arabidopsis thaliana enhances resistance to NaCl and H2O2

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