Molecular cloning and differential expression of an γ-aminobutyrate transaminase gene, OsGABA-T, in rice (Oryza sativa) leaves infected with blast fungus

Wu, Chunxia; Zhou, Shijiao; Zhang, Quan; Zhao, Wensheng; Peng, You-Liang

doi:10.1007/s10265-006-0018-3

Cited by 25 publications

(23 citation statements)

References 52 publications

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“…Glutamate levels were gradually decreased starting from the 1st day of the harpin infiltration and declined to the lowest levels of all groups. Similar to our results GABA levels were increased upon Cladosporium fulvum and P. syringae infection in tomato and A. thaliana and GABA shunt is activated by blast fungus infection in rice (Solomon and Oliver 2002;Wu et al, 2006;Park et al, 2010). Chevrot et al (2006) also found a rapid decrease in glutamate content which was associated with the increase in the GABA levels of tomato plants infected by A. tumefaciens.…”

Section: Discussionsupporting

confidence: 92%

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The impact of GABA in harpin-elicited biotic stress responses in Nicotiana tabaccum

Dimlioğlu

Daş

Bor

et al. 2015

Journal of Plant Physiology

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Section: Discussionsupporting

confidence: 92%

“…In the studies mentioned above (Solomon and Oliver 2002;Wu et al, 2006;Park et al, 2010) high levels of GABA might be related to the increased activities of GDH and GAD enzymes. Taking this into account in the present study, we measured the activities of GDH and GAD enzymes and the expression profiles of the genes encoding these enzymes.…”

Section: Discussionmentioning

confidence: 94%

The impact of GABA in harpin-elicited biotic stress responses in Nicotiana tabaccum

Dimlioğlu

Daş

Bor

et al. 2015

Journal of Plant Physiology

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“…However, the upregulation of non‐PR genes suggested that tomato plants might use additional defense responses to cope with Bt infestation. Such is the case of the GABA transaminase subunit precursor, which was found to be induced in rice ( Oryza sativa ) leaves infected with an incompatible race of Magnaporthe grisea (Wu et al 2006). A contrasting possibility is that an upregulated expression GABA transaminase leading to the degradation of GABA, a potential control agent in plant‐invertebrate pest systems (Bown et al 2006; Shelp et al 2006) might favor rather than deter Bt infestation.…”

Section: Discussionmentioning

confidence: 99%

Differential gene expression in whiteflyBemisia tabaci‐infested tomato (Solanum lycopersicum) plants at progressing developmental stages of the insect's life cycle

Estrada-Hernández

Valenzuela-Soto

Ibarra-Laclette

et al. 2009

Physiologia Plantarum

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A suppression-subtractive-hybridization (SSH) strategy was used to identify genes whose expression was modified in response to virus-free whitefly Bemisia tabaci (Bt, biotype A) infestation in tomato (Solanum lycopersicum) plants. Thus, forward and reverse SSH gene libraries were generated at four points in the whitefly's life cycle, namely at (1) 2 days (adult feeding and oviposition: phase I); (2) 7 days (mobile crawler stage: phase II); (3) 12 days (second to third instar nymphal transition: phase III) and (4) 18 days (fourth instar nymphal stage: phase IV). The 169 genes with altered expression (up and downregulated) that were identified in the eight generated SSH libraries, together with 75 additional genes that were selected on the basis of their involvement in resistance responses against phytofagous insects and pathogens, were printed on a Nexterion(®) Slide MPX 16 to monitor their pattern of expression at the above phases. The results indicated that Bt infestation in tomato led to distinctive phase-specific expression/repression patterns of several genes associated predominantly with photosynthesis, senescence, secondary metabolism and (a)biotic stress. Most of the gene expression modifications were detected in phase III, coinciding with intense larval feeding, whereas fewer changes were detected in phases I and IV. These results complement previously reported gene expression profiles in Bt-infested tomato and Arabidopisis, and support and expand the opinion that Bt infestation leads to the downregulation of specific defense responses in addition to those controlled by jasmonic acid.

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“…At GABA-T knockout mutants have been characterized and found to contain a reduced seed generation phenotype (Palanivelu et al , 2003). More recently, two homologous GABA-T genes have been identified in rice (Ansari et al , 2005; Wu et al , 2006), and another in pepper (GenBank accession number AAC78480). Interestingly, the pepper gene and one of the rice genes ( Osl2 ) lack sequences encoding an N-terminal targeting presequence, making cytosolic localization likely, although neither gene product has been tested for GABA-T activity nor has its location been confirmed experimentally.…”

Section: Introductionmentioning

confidence: 99%

Subcellular localization and expression of multiple tomato γ-aminobutyrate transaminases that utilize both pyruvate and glyoxylate

Clark

Leo

Cauwenberghe

et al. 2009

Journal of Experimental Botany

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γ-Aminobutyric acid transaminase (GABA-T) catalyses the breakdown of GABA to succinic semialdehyde. In this report, three GABA-T isoforms were identified in the tomato (Solanum lycopersicum L.) plant. The deduced amino acid sequences of the three isoforms are highly similar over most of their coding regions with the exception of their N-terminal regions. Transient expression of the individual full-length GABA-T isoforms fused to the green fluorescent protein in tobacco suspension-cultured cells revealed their distinct subcellular localizations to the mitochondrion, plastid or cytosol, and that the specific targeting of the mitochondrion- and plastid-localized isoforms is mediated by their predicted N-terminal presequences. Removal of the N-terminal targeting presequences from the mitochondrion and plastid GABA-T isoforms yielded good recovery of the soluble recombinant proteins in Escherichia coli when they were co-expressed with the GroES/EL molecular chaperone complex. Activity assays indicated that all three recombinant isoforms possess both pyruvate- and glyoxylate-dependent GABA-T activities, although the mitochondrial enzyme has a specific activity that is significantly higher than that of its plastid and cytosolic counterparts. Finally, differential expression patterns of the three GABA-T isoforms in reproductive tissues, but not vegetative tissues, suggest unique roles for each enzyme in developmental processes. Overall, these findings, together with recent information about rice and pepper GABA-Ts, indicate that the subcellular distribution of GABA-T in the plant kingdom is highly variable.

show abstract

Molecular cloning and differential expression of an γ-aminobutyrate transaminase gene, OsGABA-T, in rice (Oryza sativa) leaves infected with blast fungus

Cited by 25 publications

References 52 publications

The impact of GABA in harpin-elicited biotic stress responses in Nicotiana tabaccum

The impact of GABA in harpin-elicited biotic stress responses in Nicotiana tabaccum

Differential gene expression in whiteflyBemisia tabaci‐infested tomato (Solanum lycopersicum) plants at progressing developmental stages of the insect's life cycle

Subcellular localization and expression of multiple tomato γ-aminobutyrate transaminases that utilize both pyruvate and glyoxylate

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