Molecular cloning and expression analysis of a monoterpene synthase gene involved in floral scent production in lily (Lilium ‘Siberia’)

Zhang, T. X.; Sun, Ming; Li, L. L.; Guo, Yanhong; Xie, Xiangming; Hu, Ben

doi:10.1134/s1021443717040203

Cited by 10 publications

(4 citation statements)

References 28 publications

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“…LTPS-1 presented the highest levels of nucleotide diversity in this study, and a total of 23 LTPS-1 were identified from 78.8% of lily cultivars, including the Asiatic hybrid “Little Kiss” (faint scented), Oriental hybrid “Sorbonne” (lily scent), OT hybrid “Pink Mist” (fruity-honey scent), Trumpet hybrid “Pink Perfection” (musky scent), and LA hybrid “Golden Stone” (fruity scent) (Tables 1–3). The two TPS sequences reported from the typical lily-scented “Siberia” and “Belladonna” by Zhang et al 37 and Johnson et al 14 are members of subfamily TPS-b and were similar/identical to TPS-1 in our study (Supplementary Fig. 2).…”

Section: Discussionsupporting

confidence: 73%

“…Recently, two LiTPS genes have been cloned, one from Lilium “Siberia” (NCBI accession number KF734591), consisting of a 1761-bp open reading frame (ORF) and encoding a 587 amino acid protein 37 , and another, LhTPS cloned from “Belladonna” (NCBI accession number KR998333), with an ORF of 1758 bp corresponding to 586 amino acids 14 . However, no information is available on the genetic diversity of monoTPSs or their relationship to floral scent in lily.…”

Section: Introductionmentioning

confidence: 99%

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Volatile composition and classification of Lilium flower aroma types and identification, polymorphisms, and alternative splicing of their monoterpene synthase genes

Wang

Fan

et al. 2019

Hortic Res

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Lily is a well-known ornamental plant with a diversity of fragrant types. Basic information on lily floral scent compounds has been obtained for only a few accessions, and little is known about Lilium aroma types, the terpene synthase genes that may play roles in the production of key volatiles, or the range of monoterpenes that these genes produce. In this study, 41 cultivars were analyzed for volatile emissions, and a total of 46 individual volatile compounds were identified, 16 for the first time in lilies. Lily accessions were classified into six groups according to the composition of major scent components: faint-scented, cool, fruity, musky, fruity-honey, and lily. Monoterpenes were one of the main groups of volatiles identified, and attention was focused on terpene synthase (TPS) genes, which encode enzymes that catalyze the last steps in monoterpene synthesis. Thirty-two candidate monoterpene synthase cDNAs were obtained from 66 lily cultivars, and 64 SNPs were identified. Two InDels were also shown to result from variable splicing, and sequence analysis suggested that different transcripts arose from the same gene. All identified nucleotide substitution sites were highly correlated with the amounts of myrcene emitted, and InDel site 230 was highly correlated with the emission of all major monoterpenoid components, especially (E)-β-ocimene. Heterologous expression of five cDNAs cloned from faint-scented and strong-scented lilies showed that their corresponding enzymes could convert geranyl diphosphate to (E)-β-ocimene, α-pinene, and limonene. The findings from this study provide a major resource for the assessment of lily scent volatiles and will be helpful in breeding of improved volatile components.

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Section: Discussionsupporting

confidence: 73%

Section: Introductionmentioning

confidence: 99%

Volatile composition and classification of Lilium flower aroma types and identification, polymorphisms, and alternative splicing of their monoterpene synthase genes

Wang

Fan

et al. 2019

Hortic Res

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“…However, due to the incomplete understanding of the phenylpropanoid/phenylcyclic compound metabolic pathways, further investigations are necessary to elucidate the specific mechanisms underlying their interplay with terpene metabolic pathways. Furthermore, Zhang Tengxun [39] transferred the DXS enzyme gene, an essential component of the MEP pathway in Lilium brownii, into N. tabacum and observed significantly higher linalool content in the transgenic flowers compared to wild-type plants. Similarly, in our experiment, the introduction of the key enzyme gene HDR from the MEP pathway into N. benthamiana resulted in increased linalool content in the transgenic plants' flowers.…”

Section: Discussionmentioning

confidence: 99%

Cloning and Functional Analysis of NtHDR in ‘Jinzhanyintai’ of Narcissus tazetta var. chinensis M.Roem

Zhang

Fan

et al. 2023

Horticulturae

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The gene encoding 1-hydroxy-2-methyl-2-(E)-butenyl-4-pyrophosphate reductase, a key enzyme at the end of the methylerythritol 4-phosphate pathway (MEP), was cloned from the petals of Narcissus tazetta var. Chinensis ‘Jinzhanyintai’ and named NtHDR (accession number OQ739816). NtHDR exhibits a full-length coding sequence spanning 1380 base pairs, encoding a total of 460 amino acids. Through phylogenetic-tree analysis, it has been determined that NtHDR shared the closest evolutionary relationship with monocotyledons, specifically Asparagus officinalis and Zingiber officinale. Utilizing quantitative real-time PCR analysis, it was observed that NtHDR exhibited significant expression in both petals and corona, with expression levels varying throughout the flowering process of Narcissus. Specifically, in petals, NtHDR expression demonstrated a pattern of initial increase followed by subsequent decrease, while in corona, it consistently increased. Subsequent subcellular-localization experiments indicated that NtHDR was localized within the chloroplasts. To investigate the functional impact of NtHDR, a stable transformation was performed, where NtHDR was introduced into Nicotiana benthamiana. The resulting transgenic N. benthamiana flowers were analyzed using solid-phase microextraction coupled with gas chromatography–mass spectrometry (SPME-GC-MS) to characterize their volatile components. The analysis revealed the presence of the monoterpene compound linalool, as well as the phenylpropanoids benzyl alcohol and phenylethanol, within the floral fragrance components of the transgenic N. benthamiana plants. However, these compounds were absent in the floral fragrance components of wild-type tobacco plants, thus highlighting the impact of NtHDR transformation on the floral scent profile.

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“…However, little is known about the molecular mechanism underlying the production of these flower volatiles. To date, only two monoterpene synthases 40 , 41 and one benzoic acid/salicylic acid carboxyl methyltransferase 42 genes have been isolated from lily. In addition, recent studies have shown that scent emissions of lily is in concert with floral development stages 41 , and exhibited rhythmic patterns 37 .…”

Section: Introductionmentioning

confidence: 99%

Two-dimensional analysis provides molecular insight into flower scent of Lilium ‘Siberia’

Shi

Duan

et al. 2018

Sci Rep

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Lily is a popular flower around the world not only because of its elegant appearance, but also due to its appealing scent. Little is known about the regulation of the volatile compound biosynthesis in lily flower scent. Here, we conducted an approach combining two-dimensional analysis and weighted gene co-expression network analysis (WGCNA) to explore candidate genes regulating flower scent production. In the approach, changes of flower volatile emissions and corresponding gene expression profiles at four flower developmental stages and four circadian times were both captured by GC-MS and RNA-seq methods. By overlapping differentially-expressed genes (DEGs) that responded to flower scent changes in flower development and circadian rhythm, 3,426 DEGs were initially identified to be candidates for flower scent production, of which 1,270 were predicted as transcriptional factors (TFs). The DEGs were further correlated to individual flower volatiles by WGCNA. Finally, 37, 41 and 90 genes were identified as candidate TFs likely regulating terpenoids, phenylpropanoids and fatty acid derivatives productions, respectively. Moreover, by WGCNA several genes related to auxin, gibberellins and ABC transporter were revealed to be responsible for flower scent production. Thus, this strategy provides an important foundation for future studies on the molecular mechanisms involved in floral scent production.

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Molecular cloning and expression analysis of a monoterpene synthase gene involved in floral scent production in lily (Lilium ‘Siberia’)

Cited by 10 publications

References 28 publications

Volatile composition and classification of Lilium flower aroma types and identification, polymorphisms, and alternative splicing of their monoterpene synthase genes

Volatile composition and classification of Lilium flower aroma types and identification, polymorphisms, and alternative splicing of their monoterpene synthase genes

Cloning and Functional Analysis of NtHDR in ‘Jinzhanyintai’ of Narcissus tazetta var. chinensis M.Roem

Two-dimensional analysis provides molecular insight into flower scent of Lilium ‘Siberia’

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