Molecular cloning and expression analysis of immunoglobulin M heavy chain gene of blunt snout bream (Megalobrama amblycephala)

Hu, Xia; Wu, Kang Hsi; Liu, Wanjing; Gul, Yasmeen; Wang, Weimin; Zhang, Xuezhen

doi:10.1016/j.fsi.2014.06.026

Cited by 14 publications

(24 citation statements)

References 40 publications

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“…IgM knockout studies with mice have shown that IgD can largely substitute for IgM functions in B cells [ 39 ]. In this study, the expression patterns of mIgD in various tissues of M. amblycephala post A. hydrophila infection were in line with that of IgM [ 30 , 40 , 41 ]. Thus, mIgD may have an important role in the adaptive immune system, conferring protective functions against pathogens.…”

Section: Discussionsupporting

confidence: 64%

“…In this study, M. amblycephala mIgD was detected mainly in head kidney, trunk kidney and spleen in both juvenile and adult fish, which was consistent with the above reports. Moreover, in both juvenile and adult fish, M. amblycephala sIgM was highly expressed in head kidney, trunk kidney and spleen in our previous study [ 30 ]. It indicates that head kidney, trunk kidney and spleen are predominant immune organs.…”

Section: Discussionmentioning

confidence: 83%

“…In the present ontogeny study, mIgD mRNA was detected in zygotes, suggesting the possibility of maternal mRNA transfer into oocytes. Indeed, it was found that the level of mIgD mRNA was the highest in the unfertilized egg stage compared to other early developmental stages, which is similar to M. amblycephala sIgM [ 30 ]. In addition, the level of mIgD mRNA decreased dramatically from the unfertilized egg to zygote stage, reaching very low levels from the 2-cell to the 32-cell stages; by the prelarva stage, mIgD levels were much closer to those seen in the unfertilized egg.…”

Section: Discussionmentioning

confidence: 99%

“…In our previous study, M. amblycephala MHC I transcripts were detected to decrease from 0 to 4 h after A. hydrophila injection in gill and liver [ 43 ]. Moreover, the level of sIgM mRNA was found significantly decreased at 4 h in trunk kidney, spleen and gill of M. amblycephala after A. hydrophila infection [ 30 ]. In this study, compared with the control group, the expression pattern of M. amblycephala mIgD gene was found down-regulated at 4 h after injection in trunk kidney and spleen.…”

Section: Discussionmentioning

confidence: 99%

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Experimental immunology Spatio-temporal expression of blunt snout bream ( Megalobrama amblycephala ) mIgD and its immune response to Aeromonas hydrophila

Hu¹,

Wu²,

Liu³

et al. 2015

cejoi

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The function of IgD in fish and mammals has not been fully understood since its discovery. In this study, we have isolated and characterized the cDNA that encodes membrane-bound form of the immunoglobulin D heavy chain gene (mIgD) of blunt snout bream (Megalobrama amblycephala) using RT-PCR and rapid amplification of cDNA ends (RACE). The full-length cDNA of mIgD consisted of 3313 bp, encoding a putative protein of 943 amino acids. The structure of blunt snout bream mIgD is VDJ-μ1-δ1-δ2-δ3-δ4-δ5-δ6-δ7-TM. Multiple alignment and phylogenetic analyses indicated that blunt snout bream mIgD clusters with the homologues of cyprinid fish and that its highest identity is with that of C. idella (82%). The mIgD expression in early different developmental stages showed that the level of mIgD mRNA decreased dramatically from the unfertilized egg stage to the 32-cell stage, suggesting that mIgD mRNA was maternally transferred. As cell differentiation initially took place in the blastula stage, the mIgD expression increased significantly from the blastula stage to prelarva, which might be attributed to embryonic stem cell differentiation processes. Compared with juvenile fish, the expression and tissue distribution patterns of mIgD in adult individuals exhibited considerable variation. After the injection of Aeromonas hydrophila, mIgD expression was up-regulated in various tissues, reaching the peak expression at 5 d, 14 d or 21 d (depending on the tissue type). The present study provides a theoretical basis for further research of the teleost immune system.

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Section: Discussionsupporting

confidence: 64%

Section: Discussionmentioning

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Experimental immunology Spatio-temporal expression of blunt snout bream ( Megalobrama amblycephala ) mIgD and its immune response to Aeromonas hydrophila

Hu¹,

Wu²,

Liu³

et al. 2015

cejoi

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“…Aeromonas hydrophila infection was a major problem in the blunt snout bream culture industry in China. Until now, the effect of Aeromonas hydrophila infection on the immunity of blunt snout bream has been rarely studied, although it is very important for aquaculture [ 9 - 11 ]. Knowledge of the effect of Aeromonas hydrophila infection on non-specific immunity is of potential importance for the immunological control of disease in the M. amblycephala farms.…”

Section: Introductionmentioning

confidence: 99%

The effect of Aeromonas hydrophila infection on the non-specific immunity of blunt snout bream ( Megalobrama amblycephala )

Hu¹,

Tang²,

Lu³

et al. 2017

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Aeromonas hydrophila is the main reason of epidemic septicaemia for freshwater fish. In the present study, the effect of Aeromonas hydrophila infection on the non-specific immunity of blunt snout bream (Megalobrama amblycephala) was studied. After Aeromonas hydrophila challenge, lysozyme activity was significantly increased at 4 h, 1 d, 3 d, 5 d, 14 d and 21 d. An increased level of lysozyme activity indicated a natural protective mechanism in fish. The significant increases of superoxide dismutase activity and catalase activity in treatment group were detected at 4 h, 1 d, 3 d, 5 d, 14 d and 21 d after Aeromonas hydrophila challenge. Increase in the superoxide anion and H2O2 is considered to be beneficial for self-protection from disease. Acid phosphatase activity increased significantly at 1 d, 3 d and 5 d after Aeromonas hydrophila challenge. Alkaline phosphatase activity in treatment group showed significant increase at 4 h, 1 d, 3 d, 5 d, 14 d and 21 d compared to control group. Increased phosphatase activity indicates higher breakdown of the energy reserve, which is utilized for the growth and survival of fish. These results revealed that the non-specific immunity of fish played an important role in self-protection after pathogens infection.

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Comparison of the roles of IgM in systemic and mucosal immunity via tissue distribution analysis in largemouth bass (Micropterus salmoides)

Yang

Chen

et al. 2020

Aquaculture

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Molecular cloning and expression analysis of immunoglobulin M heavy chain gene of blunt snout bream (Megalobrama amblycephala)

Cited by 14 publications

References 40 publications

Experimental immunology Spatio-temporal expression of blunt snout bream ( Megalobrama amblycephala ) mIgD and its immune response to Aeromonas hydrophila

Experimental immunology Spatio-temporal expression of blunt snout bream ( Megalobrama amblycephala ) mIgD and its immune response to Aeromonas hydrophila

The effect of Aeromonas hydrophila infection on the non-specific immunity of blunt snout bream ( Megalobrama amblycephala )

Comparison of the roles of IgM in systemic and mucosal immunity via tissue distribution analysis in largemouth bass (Micropterus salmoides)

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