Molecular cloning and expression of Hedychium coronarium farnesyl pyrophosphate synthase gene and its possible involvement in the biosynthesis of floral and wounding/herbivory induced leaf volatile sesquiterpenoids

Cloning and characterization of a farnesyl pyrophosphate synthase from Matricaria recutita L. and its upregulation by methyl jasmonate

“…Specific accumulation of sesquiterpenoids that was consistent with FPS expression levels has been recorded in some plant species, such as W. somnifera (Gupta et al, 2011), C. praecox (Xiang et al, 2010), and H. coronarium (Lan et al, 2013). Therefore, we can speculate that, during flower development, expression levels of the MrFPS gene is correlated with sesquiterpenoid production.…”

“…Because of the crucial role of FPP, a large group of FPS genes have been cloned and characterized from a variety of plant species, including Arabidopsis (Closa et al, 2010), Artemisia annua (Matsushita et al, 1996;Hemmerlin et al, 2003), maize (Cervantes et al, 2006), Chimonanthus praecox (Xiang et al, 2010), Withania somnifera (Gupta et al, 2011), and Hedychium coronarium (Lan et al, 2013). Nevertheless, as far as we know, there was only one coding sequence of the fps gene found in M. recutita and insufficient discussion.…”

Farnesol produced by the biocontrol agent Candida ernobii can be used in controlling the postharvest pathogen Penicillium expansum

“…In this study, the strain Pg2-5 also has the potency in controlling postharvest pathogen P. expansum L. The identity of the compounds produced by strain Pg2-5 was confirmed to be farnesol. Farnesol is a 15-carbon isoprenoid alcohol widely distributed in nature as an odoriferous component that may be generated within cells by enzymatic dephosphorylation of farnesyl pyrophosphate, an intermediate of the metabolic pathway yielding sterols and other isoprenoid compounds from mevalonate (Lan et al, 2013). It has been reported that farnesol inhibits the growth of various important plant pathogenic and zoopathogenic fungi such as A. nidulans (Semighini et al, 2006), C. albicans (Shirtliff et al, 2009) and B. cinerea (Cotoras et al, 2013).…”

Histological analysis of somatic embryogenesis in pineapple: AcSERK1 and its expression validation under stress conditions

“…A single colony of an E. coli strain BL21 (DE) harboring the pET-AcSERK1 plasmid was cultured until the OD600 reached 0.5. The protocol for protein purification is as given in Lan et al (2013) and Yue et al (2014). Briefly, cell cultures were inducted with 0.1 mM isopropyl-b-Dthiogalactopyranoside (IPTG) and then incubated overnight at 16°C and 180 rpm.…”