Molecular Cloning and Functional Analysis of a Na+-Insensitive K+ Transporter of Capsicum chinense Jacq

Ruíz-Lau, Nancy; Bojórquez-Quintal, Emanuel; Benito, Begoña; Echevarría‐Machado, Ileana; Sánchez-Cach, Lucila A.; Medina-Lara, María de Fátima; Martínez‐Estévez, Manuel

doi:10.3389/fpls.2016.01980

Cited by 8 publications

(4 citation statements)

References 87 publications

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“…HAK/KUP/KT transporters could not generate K + currents in cells, thus, their function studies mainly carried out in yeast or bacteria mutants defective for K + uptake like yeast Cy162 strains. Interestingly, the HbHAK1 transformants can grow in the presence of extremely low external K + (10 μM), as the same experiments confirm HvHAK1, OsHAK5, CcHAK1 and CaHAK1 transformants recover the growth at 0.1-mM K + added [ 12 , 33 , 34 ] and OsHAK1 or AtHAK5 expressing strains can grow at 0.3-mM external K + content [ 8 , 10 ]. The best performance of HbHAK1 in complementation of Cy162 strains indicated its strong K + transport activity and extended the minimum threshold of K + uptake operated by HAK-type transporters.…”

Section: Discussionmentioning

confidence: 76%

“…Their proteins possess 10-15 transmembrane (TM) domains and are divided into 5 clusters [5]. Genes in cluster I are the most functionally revealed, including classical AtHAK5, HvHAK1, OsHAK1, OsHAK5, ThHAK1, SlHAK5, CcHAK1 and so on [6][7][8][9][10][11][12]. They all have a high-affinity K + transport feature that allows plant to thrive under low-K + (<1 mM) conditions.…”

Section: Introductionmentioning

confidence: 99%

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Halophytic Hordeum brevisubulatum HbHAK1 Facilitates Potassium Retention and Contributes to Salt Tolerance

Zhang

Xiao

et al. 2020

IJMS

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Potassium retention under saline conditions has emerged as an important determinant for salt tolerance in plants. Halophytic Hordeum brevisubulatum evolves better strategies to retain K+ to improve high-salt tolerance. Hence, uncovering K+-efficient uptake under salt stress is vital for understanding K+ homeostasis. HAK/KUP/KT transporters play important roles in promoting K+ uptake during multiple stresses. Here, we obtained nine salt-induced HAK/KUP/KT members in H. brevisubulatum with different expression patterns compared with H. vulgare through transcriptomic analysis. One member HbHAK1 showed high-affinity K+ transporter activity in athak5 to cope with low-K+ or salt stresses. The expression of HbHAK1 in yeast Cy162 strains exhibited strong activities in K+ uptake under extremely low external K+ conditions and reducing Na+ toxicity to maintain the survival of yeast cells under high-salt-stress. Comparing with the sequence of barley HvHAK1, we found that C170 and R342 in a conserved domain played pivotal roles in K+ selectivity under extremely low-K+ conditions (10 μM) and that A13 was responsible for the salt tolerance. Our findings revealed the mechanism of HbHAK1 for K+ accumulation and the significant natural adaptive sites for HAK1 activity, highlighting the potential value for crops to promote K+-uptake under stresses.

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Section: Discussionmentioning

confidence: 76%

Section: Introductionmentioning

confidence: 99%

Halophytic Hordeum brevisubulatum HbHAK1 Facilitates Potassium Retention and Contributes to Salt Tolerance

Zhang

Xiao

et al. 2020

IJMS

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“…Moreover, KUP has been previously shown to transport cesium by an AtHAK5deficient Arabidopsis mutant and an AtHAK5-producing yeast whose phenotypes showed lower Cs+ accumulation and higher cesium uptake, respectively [26]. The role of KIRCs in cesium uptake is not clear because these potassium channels are severely inhibited by the transport of Cs ions, which act as potassium channel blockers and close the channel [23,27].…”

Section: Cesiummentioning

confidence: 99%

Bioremediation of Biophilic Radionuclides by Algae

Minoda¹

2019

Algae

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High amounts of radionuclides were released into the environment by the nuclear power plant accident of 2011 in Japan. Among the radioactive material, cesium, iodine, and strontium were especially dangerous because of their biophilic characteristics that allowed them to accumulate in living organisms, either as essential elements for iodine or analogs of potassium and calcium for cesium and strontium, respectively. As a result, there was a high social demand for decontamination to avoid exposure to these elements. The authors screened around 200 strains of algae and plants for their ability to absorb radioactive nuclides. The eustigmatophycean algae Vacuoliviride crystalliferum and the cyanophytes Stigonema ocellatum and Nostoc commune showed the highest bioaccumulation activity for the removal of cesium, strontium, and iodine from the environment, respectively. In addition to these strains, the authors also found that the extremophilic unicellular red algae Galdieria sulphuraria could remove high levels of dissolved cesium from media in mixotrophic growth conditions. In this chapter, the intake mechanism of cesium, iodine, and strontium is reviewed. Recent findings on the absorption of these elements by algae are discussed to highlight the possibility of decontaminating polluted land and water at nuclear sites by phytoremediation.

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“…There are mainly two kinds of transport systems in plants, (a) through channels or (b) via transporters ( Adams and Shin, 2014 ; Ruiz-Lau et al, 2016 ). Four multi-gene K + transporter families are found in plants, (i) KUP/HAK/KT, (ii) HKT/Trk, (iii) CHX, and (iv) KEA transporters ( Véry and Sentenac, 2003 ; Sharma et al, 2013 ; Yang et al, 2014 ; Gupta et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Regulation of AtKUP2 Expression by bHLH and WRKY Transcription Factors Helps to Confer Increased Salt Tolerance to Arabidopsis thaliana Plants

2020

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Potassium transporters play an essential role in maintaining cellular ion homeostasis, turgor pressure, and pH, which are critical for adaptation under salt stress. We identified a salt responsive Avicennia officinalis KUP/HAK/KT transporter family gene, AoKUP2 , which has high sequence similarity to its Arabidopsis ortholog AtKUP2 . These genes were functionally characterized in mutant yeast cells and Arabidopsis plants. Both AoKUP2 and AtKUP2 were induced by salt stress, and AtKUP2 was primarily induced in roots. Subcellular localization revealed that AoKUP2 and AtKUP2 are localized to the plasma membrane and mitochondria. Expression of AtKUP2 and AoKUP2 in Saccharomyces cerevisiae mutant strain (BY4741 trk1 Δ ::loxP trk2 Δ ::loxP ) helped to rescue the growth defect of the mutant under different NaCl and K + concentrations. Furthermore, constitutive expression of AoKUP2 and AtKUP2 conferred enhanced salt tolerance in Arabidopsis indicated by higher germination rate, better survival, and increased root and shoot length compared to the untreated controls. Analysis of Na + and K + contents in the shoots and roots showed that ectopic expression lines accumulated less Na + and more K + than the WT. Two stress-responsive transcription factors, bHLH122 and WRKY33, were identified as direct regulators of AtKUP2 expression. Our results suggest that AtKUP2 plays a key role in enhancing salt stress tolerance by maintaining cellular ion homeostasis.

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Molecular Cloning and Functional Analysis of a Na+-Insensitive K+ Transporter of Capsicum chinense Jacq

Cited by 8 publications

References 87 publications

Halophytic Hordeum brevisubulatum HbHAK1 Facilitates Potassium Retention and Contributes to Salt Tolerance

Halophytic Hordeum brevisubulatum HbHAK1 Facilitates Potassium Retention and Contributes to Salt Tolerance

Bioremediation of Biophilic Radionuclides by Algae

Regulation of AtKUP2 Expression by bHLH and WRKY Transcription Factors Helps to Confer Increased Salt Tolerance to Arabidopsis thaliana Plants

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