Molecular cloning and functional characterization of V<sub>2</sub> [8‐lysine] vasopressin and oxytocin receptors from a pig kidney cell line

Gorbulev, Valentin; Büchner, Hubert; Akhundova, Aida; Fahrenholz, Falk

doi:10.1111/j.1432-1033.1993.tb18000.x

Cited by 96 publications

(70 citation statements)

References 41 publications

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“…The structure of the OTR has been elucidated by molecular cloning in different mammalian species (Kimura et al 1992, Gorbulev et al 1993, Bathgate et al 1995, Riley et al 1995, Rozen et al 1995. The deduced amino acid sequences indicated that this receptor belongs to the G protein-coupled receptor family.…”

Section: Introductionmentioning

confidence: 99%

Oxytocin receptor gene expression in rat mammary gland: structural characterization and regulation

Breton

Guenot²,

Zingg

2001

Journal of Molecular Endocrinology

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The differential, tissue-specific regulation of oxytocin (OT) binding sites allows the neurohypophysial nonapeptide OT to fulfill a dual role: to induce uterine contractions at parturition and to mediate milk ejection during lactation. Whereas uterine OT binding sites are up-regulated prior to parturition and are rapidly down-regulated thereafter, mammary gland OT binding sites gradually increase throughout gestation and remain up-regulated during the ensuing lactation period. Here, we structurally characterized OT receptor (OTR) mRNA in mammary gland and analyzed its expression during gestation and lactation and in response to steroid treatment. In mammary gland tissues, we found a 6·7 and a 5·4 kb OTR mRNA species, and both species were further analyzed by RACE (rapid amplification of cDNA ends). The 6·7 kb mRNA was found to be common to mammary gland and uterus and to extend 618 nucleotides beyond the published sequence of the rat OTR gene. The 5·4 kb mRNA species is unique to the mammary gland and terminates at a mammary gland-specific polyadenylation site that is not preceded by a classical polyadenylation signal. RT-PCR analysis did not provide any evidence for differences in the coding regions, suggesting that both uterine and mammary gland OTR mRNAs encode the same receptor protein. Furthermore, primer extension experiments showed that no differences exist in the specific transcriptional initiation sites of the OTR gene in the two tissues. During pregnancy, OTR mRNA per mammary gland increased approximately 150-fold and remained high during lactation, consistent with the previously identified regulation of OT binding sites and the role of OT during lactation. Whereas estrogen administration strongly induced the uterine OTR mRNA levels (>5-fold), mammary gland remained unaffected by steroid treatment. Moreover, tamoxifen had no effect on the mammary gland OTR mRNA level. In summary, our data demonstrate a differential control of OTR expression in uterus versus mammary gland and a mammary gland-specific OTR mRNA polyadenylation site. However, this differential control apparently does not involve the expression of different receptor genes nor the utilization of tissue-specific transcriptional initiation sites.

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Section: Introductionmentioning

confidence: 99%

Oxytocin receptor gene expression in rat mammary gland: structural characterization and regulation

Breton

Guenot²,

Zingg

2001

Journal of Molecular Endocrinology

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“…GPCRs mediate the actions of numerous neurotransmitters and hormones (Probst et al 1992). The V2 receptor mediates the antidiuretic effects of VP and couples positively to adenylate cyclase (Lolait et al 1992, Gorbulev et al 1993. The OT receptor (Kimura et al 1992, Gorbulev et al 1993 mediates various central and peripheral functions in reproduction in mammals.…”

Section: Introductionmentioning

confidence: 99%

“…The V2 receptor mediates the antidiuretic effects of VP and couples positively to adenylate cyclase (Lolait et al 1992, Gorbulev et al 1993. The OT receptor (Kimura et al 1992, Gorbulev et al 1993 mediates various central and peripheral functions in reproduction in mammals. V1a (Thibonnier et al 1994) and V1b (Sugimoto et al 1994) receptors mediate the effects of VP on liver glycogenolysis and the release of adrenocorticotropin, respectively.…”

Section: Introductionmentioning

confidence: 99%

Cloning of Octopus cephalotocin receptor, a member of the oxytocin/vasopressin superfamily

Kanda

Takuwa‐Kuroda²,

Ukena³

et al. 2003

Journal of Endocrinology

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We reported that the common octopus, Octopus vulgaris, in common with vertebrates, possesses two members of the oxytocin/vasopressin superfamily: octopressin (OP) and cephalotocin (CT). This was the first observation of its kind in invertebrates. As OP and CT have different biological activities, the presence of specific receptors has been proposed. We cloned the cDNA of an orphan receptor from Octopus brain and found it to encode a polypeptide of 397 amino acids that displays sequences characteristic of G-protein coupled receptors. The orphan receptor showed high homology to receptors of the oxytocin/vasopressin superfamily and seemed to conserve the agonist-binding pocket common to the oxytocin and vasopressin receptors. Xenopus oocytes that express the orphan receptor responded to the application of CT by an induction of membrane Cl currents coupled to the inositol phosphate/Ca 2+ pathway. OP and the other members of the oxytocin/vasopressin superfamily did not activate this receptor. HPLC fractionation of the Octopus brain extract combined with an oocyte assay yielded a single substance that was identical to CT. On the basis of these results, we conclude that the cloned receptor is the CT receptor (CTR). Expression of CTR mRNA in Octopus was detected in the central and the peripheral nervous systems, the pancreas, the oviduct and the ovary. This receptor may mediate physiological functions of CT in Octopus such as neurotransmission, reproduction and metabolism.

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“…So far, three AVP receptors subtypes, V 1a , V 1b , and V 2 have been identified based upon their primary structure (3)(4)(5)(6)(7)(8)(9)(10), their coupling mechanisms, their tissular distributions and their pharmacological properties (for review see 11,12). The V 2 receptor belongs to the seven transmembrane G proteincoupled receptor superfamily and is positively coupled to a Gs/ adenylyl cyclase system.…”

Section: Introductionmentioning

confidence: 99%

Characterization of SR 121463A, a highly potent and selective, orally active vasopressin V2 receptor antagonist.

Gal¹,

Lacour²,

Valette³

et al. 1996

J. Clin. Invest.

239

136

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Molecular cloning and functional characterization of V₂ [8‐lysine] vasopressin and oxytocin receptors from a pig kidney cell line

Abstract: [Arg*]vasopressin and oxytocin are the two main members of the neurohypophysial hormone family found to be present in nearly all mammals.

Cited by 96 publications

References 41 publications

Oxytocin receptor gene expression in rat mammary gland: structural characterization and regulation

Oxytocin receptor gene expression in rat mammary gland: structural characterization and regulation

Cloning of Octopus cephalotocin receptor, a member of the oxytocin/vasopressin superfamily

Characterization of SR 121463A, a highly potent and selective, orally active vasopressin V2 receptor antagonist.

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Molecular cloning and functional characterization of V2 [8‐lysine] vasopressin and oxytocin receptors from a pig kidney cell line

Abstract: [Arg*]vasopressin and oxytocin are the two main members of the neurohypophysial hormone family found to be present in nearly all mammals.

Cited by 96 publications

References 41 publications

Oxytocin receptor gene expression in rat mammary gland: structural characterization and regulation

Oxytocin receptor gene expression in rat mammary gland: structural characterization and regulation

Cloning of Octopus cephalotocin receptor, a member of the oxytocin/vasopressin superfamily

Characterization of SR 121463A, a highly potent and selective, orally active vasopressin V2 receptor antagonist.

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Molecular cloning and functional characterization of V₂ [8‐lysine] vasopressin and oxytocin receptors from a pig kidney cell line