Molecular cloning and functional characterization of Argonaute-3 gene from Penaeus monodon

Phetrungnapha, Amnat; Ho, Teerapong; Udomkit, Apinunt; Panyim, Sakol; Ongvarrasopone, Chalermporn

doi:10.1016/j.fsi.2013.06.025

Cited by 21 publications

(9 citation statements)

References 47 publications

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“…Another related gene, named PmAgo3 was identified in P. monodon sharing a high identity (83%) with L. vannamei Ago2 (Phetrungnapha et al . ). TRBPs have been identified in Fenneropenaeus chinensis and M. japonicus , and alignment analysis revealed a high sequence similarity between them (99.42% identity) (Wang et al .…”

Section: Microrna Biogenesis In Penaeid Shrimpmentioning

confidence: 99%

“…In P. monodon, two Ago genes: PmAgo1 and PmAgo2 were identified and characterized (Unajak et al 2006;Dechklar et al 2008;Yang et al 2014a), whereas L. vannamei was reported to contain two Ago proteins (Lv-Ago 1 and Lv-Ago 2) (Labreuche et al 2010). Another related gene, named PmAgo3 was identified in P. monodon sharing a high identity (83%) with L. vannamei Ago2 (Phetrungnapha et al 2013). TRBPs have been identified in Fenneropenaeus chinensis and M. japonicus, and alignment analysis revealed a high sequence similarity between them (99.42% identity) (Wang et al 2009.…”

Section: Microrna Biogenesis In Penaeid Shrimpmentioning

confidence: 99%

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The role of shrimp microRNAs in immune response and beyond

González-Duarte¹,

García‐Carreño²

2018

Reviews in Aquaculture

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MicroRNAs are a class of small non‐coding RNAs that regulate gene expression at post‐transcriptional level. Regulatory RNAs were discovered in the nematode Caenorhabditis elegans, since then, their biological functions have been studied on different animals, including marine organisms. Several species of penaeid shrimp are important in ecology and fishing, including aquaculture. Overcrowding in aquaculture calls for infectious diseases, which threaten the development of shrimp aquaculture worldwide. Given that microRNAs play crucial regulatory roles in a wide variety of biological processes, there is a scientific interest to understand their contribution on shrimp physiology and pathology, specifically during immune and stress response. Increasing evidence has shown that the expression of microRNAs is affected during virus or bacterial infection and upon stress in shrimp. This information provides valuable insights for a better understanding of shrimp biology by means of microRNA regulation to bacterial and viral diseases.

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Section: Microrna Biogenesis In Penaeid Shrimpmentioning

confidence: 99%

Section: Microrna Biogenesis In Penaeid Shrimpmentioning

confidence: 99%

The role of shrimp microRNAs in immune response and beyond

González-Duarte¹,

García‐Carreño²

2018

Reviews in Aquaculture

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“…As PmTSN interacts with PmAgo‐1, but not with PmAgo‐2 or PmAgo‐3, this nuclease could be one of the components of PmAgo‐1‐RISC, indicating the involvement of PmAgo‐1 and PmTSN in the shrimp RNAi pathway (Phetrungnapha et al . ). Furthermore, Chen et al .…”

Section: Molecular Mechanismmentioning

confidence: 99%

RNA interference in shrimp and potential applications in aquaculture

Nguyen

Christiaens

Bossier

et al. 2016

Reviews in Aquaculture

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As the RNA interference mechanism was discovered, researchers have made huge strides in unravelling biological mechanisms at the gene level in a wide range of organisms. RNAi is a simple and rapid method that allows silencing gene expression at the post-transcriptional level and is triggered by the introduction of double-stranded RNA into the cell, leading to the degradation of the corresponding mRNA. In this review, we will provide an overview of successful RNAi experiments to date in Crustacea, and shrimp in particular, with special focus on various potential applications of RNA interference in research and aquaculture. An important aspect of RNAi in any species is the delivery of the double-stranded RNA and the barriers that have to be overcome to get the dsRNA into the target cells. Different options, experimental procedures, success rates and challenges in using RNAi are discussed in this review

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“…The PAZ domain contains an oligonucleotide-binding fold at the 3′-end of RNA and can bind to siRNAs [ 7 ]. The MID domain generates an associating pocket for fixing 5′-phosphate of the small RNAs [ 8 ], while PIWI domain is orthologous to ribonuclease H (RNase H) and encloses the slicing residues Asp-Asp-His (DDH), which is significant for the endonucleolytic “cleavage” action of the RISC [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

“…Ago proteins are divided into three groups of Piwi subfamily, Ago subfamily, and worm-specific Argonautes (WAGO) [ 14 ]. In Penaeus monodon , PmAgo3 is found within the Ago subfamily [ 9 ], while in D. melanogaster [ 15 ] and B. mori [ 16 ], the Ago3 protein is placed in the Piwi subfamily. Ago3 protein is related to the piRNA-mediated pathway and silences transposable elements (TEs) in D. melanogaster [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization and the Function of Argonaute3 in RNAi Pathway of Plutella xylostella

Hameed

Zheng-bing

Vasseur

et al. 2018

IJMS

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Argonaute (Ago) protein family plays a key role in the RNA interference (RNAi) process in different insects including Lepidopteran. However, the role of Ago proteins in the RNAi pathway of Plutella xylostella is still unknown. We cloned an Argonaute3 gene in P. xylostella (PxAgo3) with the complete coding sequence of 2832 bp. The encoded protein had 935 amino acids with an expected molecular weight of 108.9 kDa and an isoelectric point of 9.29. It contained a PAZ (PIWI/Argonaute/Zwile) domain and PIWI (P-element-induced whimpy testes) domain. PxAgo3 was classified into the Piwi subfamily of Ago proteins with a high similarity of 93.0% with Bombyx mori Ago3 (BmAgo3). The suppression of PxAgo3 by dsPxAgo3 was observed 3 h after treatment and was maintained until 24 h. Knockdown of PxAgo3 decreased the suppression level of PxActin by dsPxActin in P. xylostella cells, while overexpression of PxAgo3 increased the RNAi efficiency. Our results suggest that PxAgo3 play a key role in the double stranded RNA (dsRNA)-regulated RNAi pathway in P. xylostella.

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Molecular cloning and functional characterization of Argonaute-3 gene from Penaeus monodon

Cited by 21 publications

References 47 publications

The role of shrimp microRNAs in immune response and beyond

The role of shrimp microRNAs in immune response and beyond

RNA interference in shrimp and potential applications in aquaculture

Molecular Characterization and the Function of Argonaute3 in RNAi Pathway of Plutella xylostella

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