Molecular cloning and nucleotide sequence of hog cholera virus strain brescia and mapping of the genomic region encoding envelope protein E1

Moormann, R.J.M.; Warmerdam, Petra A. M.; Meer, Bert van der; Schaaper, W.M.M.; Wensvoort, G.; Hulst, M.M.

doi:10.1016/0042-6822(90)90472-4

Cited by 211 publications

(144 citation statements)

References 36 publications

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“…As an effective vaccine has to induce protection against antigenically different virus strains it should contain conserved immunogenic epitopes. Alignment of the amino acid sequences for several CSFV (Meyers et al, 1989;Moormann et al, 1990;Mfiller, 1993) and bovine viral diarrhoea virus (BVDV) strains (Collett et al, 1988 a;De Moerlooze et al, 1993;N. Tautz, unpublished data) showed that the identified T cell epitope is highly conserved among pestiviruses.…”

Section: Discussionmentioning

confidence: 99%

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Classical swine fever virus-specific cytotoxic T lymphocytes and identification of a T cell epitope

Pauly¹,

Elbers²,

König³

et al. 1995

Journal of General Virology

113

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Section: Discussionmentioning

confidence: 99%

“…The genomes of pestiviruses consist of a single-stranded RNA of positive polarity which encodes one polyprotein (Collett et al, 1988 a;Meyers et al, 1989;Moormann et al, 1990). Co-and posttranslational processing gives rise to the individual viral proteins.…”

Section: Introductionmentioning

confidence: 99%

Classical swine fever virus-specific cytotoxic T lymphocytes and identification of a T cell epitope

Pauly¹,

Elbers²,

König³

et al. 1995

Journal of General Virology

113

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“…CSFV specific primers were designed considering sequences of the 5'NTR of the genome of 55 different pestivirus strains, available in GenBank or in the database of CSF European reference laboratory of Hanover (Germany), that includes 37 CSFV representing subgroups described by Lowings [20]: 1.1, 1.2, 2.1, 2.2, 2.3 and disparate virus outgrouped [12,13,15,25,27,[31][32][33], fourteen BVDV belonging to types I and II [5,8,32] and four BDV isolates [4,29]. Sequences were aligned, using Clustal W software, and five oligonucleotides were primarily selected from highly conserved sequences among the different CSFV strains but showing high divergence with the other pestiviruses.…”

Section: Primers and Restriction Endonuclease Selectionmentioning

confidence: 99%

A highly sensitive and specific gel-based multiplex RT-PCR assay for the simultaneous and differential diagnosis of African swine fever and Classical swine fever in clinical samples

Agüero

Fernández²,

Romero

et al. 2004

Vet. Res.

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-The development and standardisation of a novel, highly sensitive and specific one-step hot start multiplex RT-PCR assay is presented for the simultaneous and differential diagnosis of African swine fever (ASF) and Classical swine fever (CSF). The method uses two primer sets, each one specific for the corresponding virus, amplifying DNA fragments different in length, allowing a gel-based differential detection of the PCR products. Universal detection of ASF and CSF virus strains was achieved through selection of primers in conserved viral genome regions. The detection range was confirmed by analysis of a large collection of isolates of the two viruses. The high specificity of the assay was proven by testing related viruses, uninfected cell line cultures and healthy pig tissues. Additional confirmatory tests of the ASF and CSF virus amplicon specificity, based on restriction endonuclease analysis with BsmA I or Ban II, respectively, are also described. The analysis of whole blood and serum samples from experimentally infected animals proved the usefulness of the method for an early diagnosis of both diseases, even before the appearance of the first clinical signs. A study of 150 positive field samples from several ASF and CSF outbreaks showed the suitability of this method for a rapid (less than five hours), sensitive and specific differential diagnosis in clinical samples. In addition, a highly sensitive and specific uniplex RT-PCR for CSFV was also developed and standardised as a powerful tool for fast and early diagnosis of the disease.

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“…They occur worldwide and are the causative agents of economically important animal diseases such as classical swine fever, bovine viral diarrhoea and border disease of sheep. Like other members of the family, pestiviruses are plus-stranded RNA viruses whose genome comprises one long open reading frame (Collett et al, 1988 ;Meyers et al, 1989 ;Moormann et al, 1990 ;Deng et al, 1992). Translation into one hypothetical polyprotein is accompanied by proteolytic processing through host cell and virus-encoded proteases.…”

Section: Introductionmentioning

confidence: 99%

Localization of pestiviral envelope proteins E(rns) and E2 at the cell surface and on isolated particles.

Weiland

Unger

et al. 1999

Journal of General Virology

100

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The glycoproteins E rns of classical swine fever virus (CSFV) and E rns and E2 of bovine viral diarrhoea virus (BVDV) are shown to be located at the surface of infected cells by the use of indirect immunofluorescence and by cytofluorometric analysis. The positive immunostaining of the cell surface was further analysed by immunogold electron microscopy and it could be shown that only extracellular virions were labelled. Gold granules were not seen at the cellular plasma membrane. In contrast to BVDV E2, the CSFV E2 of virions sticking to the plasma membrane was not accessible to the respective monoclonal antibodies. However, CSFV particles isolated from culture supernatant were able to bind both monoclonal anti-E rns and anti-E2 antibodies. For CSFV and BVDV, binding of anti-E rns antibodies to the virions was more pronounced than that of anti-E2. This finding was unexpected since E2 is considered to be the immunodominant glycoprotein.

show abstract

Molecular cloning and nucleotide sequence of hog cholera virus strain brescia and mapping of the genomic region encoding envelope protein E1

Cited by 211 publications

References 36 publications

Classical swine fever virus-specific cytotoxic T lymphocytes and identification of a T cell epitope

Classical swine fever virus-specific cytotoxic T lymphocytes and identification of a T cell epitope

A highly sensitive and specific gel-based multiplex RT-PCR assay for the simultaneous and differential diagnosis of African swine fever and Classical swine fever in clinical samples

Localization of pestiviral envelope proteins E(rns) and E2 at the cell surface and on isolated particles.

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