Molecular cloning and promoter analysis of the specific salicylic acid biosynthetic pathway gene phenylalanine ammonia‐lyase (<i>AaPAL1</i>) from<i>Artemisia annua</i>

Zhang, Ying; Fu, Xueqing; Hao, Xiaolong; Zhang, Lida; Wang, Luyao; Qian, Hongmei; Zhao, Jingya

doi:10.1002/bab.1403

Cited by 18 publications

(16 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Studies have shown that PAL is an entry-point enzyme which can convert L -Phe into trans-cinnamic acid and that it plays a vital role in channeling carbon flux from primary metabolism into the phenylpropanoid pathway [78]. So far, PAL gene has been cloned from many medicinal plants, such as Ocimum basilicum [79], Ginkgo biloba [80], Salvia miltiorrhiza [81], and A. annua [82]. In G. biloba , the highest expression of GbPAL gene was found in leaves, followed by stems, and the lowest expression was in roots; transcription levels of GbPAL were closely related to flavonoid accumulation [80].…”

Section: Discussionmentioning

confidence: 99%

Analysis of Centranthera grandiflora Benth Transcriptome Explores Genes of Catalpol, Acteoside and Azafrin Biosynthesis

Zhang

Wang

et al. 2019

IJMS

View full text Add to dashboard Cite

Cardiovascular diseases (CVDs) are a major cause of health loss in the world. Prevention and treatment of this disease by traditional Chinese medicine is a promising method. Centranthera grandiflora Benth is a high-value medicinal herb in the prevention and treatment of CVDs; its main medicinal components include iridoid glycosides, phenylethanoid glycosides, and azafrin in roots. However, biosynthetic pathways of these components and their regulatory mechanisms are unknown. Furthermore, there are no genomic resources of this herb. In this article, we provide sequence and transcript abundance data for the root, stem, and leaf transcriptome of C. grandiflora Benth obtained by the Illumina Hiseq2000. More than 438 million clean reads were obtained from root, stem, and leaf libraries, which produced 153,198 unigenes. Based on databases annotation, a total of 557, 213, and 161 unigenes were annotated to catalpol, acteoside, and azafrin biosynthetic pathways, respectively. Differentially expressed gene analysis identified 14,875 unigenes differentially enriched between leaf and root with 8,054 upregulated genes and 6,821 downregulated genes. Candidate MYB transcription factors involved in catalpol, acteoside, and azafrin biosynthesis were also predicated. This work is the first transcriptome analysis in C. grandiflora Benth which will aid the deciphering of biosynthesis pathways and regulatory mechanisms of active components.

show abstract

Section: Discussionmentioning

confidence: 99%

Analysis of Centranthera grandiflora Benth Transcriptome Explores Genes of Catalpol, Acteoside and Azafrin Biosynthesis

Zhang

Wang

et al. 2019

IJMS

View full text Add to dashboard Cite

show abstract

“…Subsequently, the enzyme benzoic-acid-2-hydroxylase (BA2H) catalyzes the hydroxylation of BA aromatic ring and leads to SA formation. The enzymatic conversion of BA into SA by BA2H requires the presence of hydrogen peroxide (H 2 O 2 ) [10][11][12].…”

Section: Introductionmentioning

confidence: 99%

The Role of Salicylic Acid in Plants Exposed to Heavy Metals

Sidhu²,

et al. 2020

View full text Add to dashboard Cite

Salicylic acid (SA) is a very simple phenolic compound (a C7H6O3 compound composed of an aromatic ring, one carboxylic and a hydroxyl group) and this simplicity contrasts with its high versatility and the involvement of SA in several plant processes either in optimal conditions or in plants facing environmental cues, including heavy metal (HM) stress. Nowadays, a huge body of evidence has unveiled that SA plays a pivotal role as plant growth regulator and influences intra- and inter-plant communication attributable to its methyl ester form, methyl salicylate, which is highly volatile. Under stress, including HM stress, SA interacts with other plant hormones (e.g., auxins, abscisic acid, gibberellin) and promotes the stimulation of antioxidant compounds and enzymes thereby alerting HM-treated plants and helping in counteracting HM stress. The present literature survey reviews recent literature concerning the roles of SA in plants suffering from HM stress with the aim of providing a comprehensive picture about SA and HM, in order to orientate the direction of future research on this topic.

show abstract

“…et al, 2015). Phenylalanine ammonia lyase ( PAL ), a rate-limiting enzyme of the phenylpropanoid pathway, is the key enzyme in the biosynthetic pathway of SA and plays an important role in plant development and defense (Hu et al, 2009; Wang et al, 2013; Zhang et al, 2016). The abundance of PAL , PR1 , and PR5 transcripts was significantly increased in the cucumber leaves infested with P. solenopsis only after 3 days, but not in the leaves infested with T. cinnabarinus only ( P < 0.05; Figure 4).…”

Section: Resultsmentioning

confidence: 99%

Plant Defense Responses Induced by Two Herbivores and Consequences for Whitefly Bemisia tabaci

Lin

Liu

et al. 2019

Front. Physiol.

View full text Add to dashboard Cite

Diverse herbivores are known to induce various plant defenses. The plant defenses may detrimentally affect the performance and preference to subsequent herbivores on the same plant, such as affecting another insect’s feeding, settling, growth or oviposition. Here, we report two herbivores (mealybug Phenacoccus solenopsis and carmine spider mite Tetranychus cinnabarinus ) which were used to pre-infest the cucumber to explore the impact on the plants and the later-colonizing species, whitefly Bemisia tabaci . The results showed that the whiteflies tended to select the treatments pre-infested by the mites, rather than the uninfected treatments. However, the result of treatments pre-infested by the mealybugs was opposite. Total number of eggs laid of whiteflies was related to their feeding preference. The results also showed that T. cinnabarinus were more likely to activate plant jasmonic acid (JA) regulated genes, while mealybugs were more likely to activate key genes regulated by salicylic acid (SA). The different plant defense activities on cucumbers may be one of the essential factors that affects the preference of B. tabaci . Moreover, the digestive enzymes and protective enzymes of the whitefly might play a substantial regulatory role in its settling and oviposition ability.

show abstract

Molecular cloning and promoter analysis of the specific salicylic acid biosynthetic pathway gene phenylalanine ammonia‐lyase (AaPAL1) fromArtemisia annua

Cited by 18 publications

References 29 publications

Analysis of Centranthera grandiflora Benth Transcriptome Explores Genes of Catalpol, Acteoside and Azafrin Biosynthesis

Analysis of Centranthera grandiflora Benth Transcriptome Explores Genes of Catalpol, Acteoside and Azafrin Biosynthesis

The Role of Salicylic Acid in Plants Exposed to Heavy Metals

Plant Defense Responses Induced by Two Herbivores and Consequences for Whitefly Bemisia tabaci

Contact Info

Product

Resources

About