Molecular cloning and sequence analysis of the <i>Zygosaccharomyces rouxii</i><i>LEU2</i> gene encoding a β‐isopropylmalate dehydrogenase

Sychrová, Hana

doi:10.1002/yea.750

Cited by 7 publications

(3 citation statements)

References 22 publications

(19 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…From the ATCC 42 981 strain, we chose two DNA fragments containing the alleles of genes encoding for the Na + /H + antiporters ZrSOD2 and ZrSOD22 . From the ATCC 2623 strain, we chose five previously sequenced DNA fragments: ADE2 (Sychrova et al , 1999); HIS3 (Sychrova et al , 2000b); LEU2 (Sychrova, 2001); HOG1 (Kinclova et al , 2001) and Zr10‐4 (Sychrova et al , 2000a). Four of these seven fragments comprised more than one gene—the ADE2 fragment consisted of ZrADE2, ZrRGA1 and part of the ZrCPF1 gene; the HIS3 fragment of ZrPET56, ZrHIS3 and part of the ZrMRP51 gene; the LEU2 fragment of ZrLEU2, ZrNSF1 , the Zr10‐4 fragment of the ZrRGD1 gene and two ORFs with homology to S. cerevisiae ORFs of unknown function.…”

Section: Resultsmentioning

confidence: 99%

Ionic liquid aqueous solvent‐based microwave‐assisted hydrolysis for the extraction and HPLC determination of myricetin and quercetin from Myrica rubra leaves

Xiao

2011

Biomedical Chromatography

View full text Add to dashboard Cite

An ionic liquid aqueous solvent-based microwave-assisted hydrolysis (ILAS-MAH) approach was proposed for the rapid extraction and accurate determination of myricetin and quercetin from Myrica rubra (M. rubra) leaves for the first time. The effects of the ionic liquid class and concentration, liquid-solid ratio, hydrolysis temperature and time were investigated to obtain the optimal ILAS-MAH conditions. The optimized conditions were 2.0 mol/L [bmin][HSO4] or 2.5 mol/L acidified [bmin]Br solution, liquid-solid ratio 30:1 (mL : g), hydrolysis temperature 70°C and hydrolysis time 10 min. Under these conditions, the recoveries of myricetin and quercetin were in the range of 86.3-107.3% with relative standard deviation lower than 5.8%. Compared with conventional heating hydrolysis and regular MAH, the proposed approach reduced hydrolysis time and improved yields. The mechanism of ILAS-MAH was also investigated.

show abstract

Section: Resultsmentioning

confidence: 99%

Ionic liquid aqueous solvent‐based microwave‐assisted hydrolysis for the extraction and HPLC determination of myricetin and quercetin from Myrica rubra leaves

Xiao

2011

Biomedical Chromatography

View full text Add to dashboard Cite

show abstract

“… Percentage sequence similarities comparison of the Leu2p sequences was made with C. milleri (translated from the nucleotide sequence deposited in the Gene Bank by Accession No. AY571 337), S. naganishii BAB63465 (Hisatomi et al , 1999), K. marxianus S32969 (Bergkamp et al , 1991), K. lactis CAA46514 (Zhang et al , 1992), C. glabrata AAB62089 (Kitada, 1997), S. cerevisiae P04173 (Andreadis et al , 1984), P. anomala CAC08508 (De la Rosa et al , 2001), Z. bailii CAB99456 (Rodrigues et al , 2001), Z. rouxii AAK82822 (Sychrova, 2001), C. boidinii Q01987 (Sakai and Tani, 1992), Y. ohmeri CAA84484 (Piredda and Gaillardin, 1994), P. angusta AAA19109 (Agaphonov et al , 1994), P. stipitis U83 626 (Lu et al , 1998), C. rugosa AJ279020 (Biasio, 2000), C. maltosa S48225 (Becher et al , 1994), C. albicans AAB58312 (Plant, 1997), Y. lipolytica AF260230 (Rojas Quijano et al , 2000), Sz. pombe T38621 (Leu1p; McLean et al , 1997), D. occidentalis CAA56224 (Iserentant and Verachtert, 1995).…”

Section: Resultsmentioning

confidence: 99%

Cloning, sequencing and application of the LEU2 gene from the sour dough yeast Candida milleri

Turakainen

Korhola

2005

Yeast

View full text Add to dashboard Cite

We have cloned by complementation in Saccharomyces cerevisiae and sequenced a LEU2 gene from the sour dough yeast Candida milleri CBS 8195 and studied its chromosomal location. The LEU2 coding sequence was 1092 nt long encoding a putative β-isopropylmalate dehydrogenase protein of 363 amino acids. The nucleotide sequence in the coding region had 71.6% identity to S. cerevisiae LEU2 sequence. On the protein level, the identity of C. milleri Leu2p to S. cerevisiae Leu2p was 84.1%. The DNA sequence of the C. milleri LEU2 gene was submitted to GenBank and assigned Accession No. AY571337. The CmLEU2 DNA probe hybridized to one to three chromosomal bands and two or three BamHI restriction fragments in C. milleri but did not give any signal to chromosomes or restriction fragments of C. albicans, S. cerevisiae, S. exiguus or Torulaspora delbrueckii. Using CmLEU2 probe for DNA hybridization makes it easy to quickly identify C. milleri among other sour dough yeasts.

show abstract

“…Particular LEU2 gene products were found to complement the leucine auxotrophy in a range of yeast species and in E. coli ( leuB mutation). This provides the option to use a single gene as a selection marker in several yeast systems [16,29,30].…”

Section: Introductionmentioning

confidence: 99%

The gene ? a new component for an -based expression platform

Wartmann

Stoltenburg

Böer

et al. 2003

FEMS Yeast Research

View full text Add to dashboard Cite

The ALEU2 gene, encoding beta-isopropylmalate dehydrogenase, was isolated from the non-conventional yeast Arxula adeninivorans. The isolated gene harbours an open reading frame of 1086 bp, encoding a putative protein of 362 amino acids. The derived protein sequence shares a high degree of homology with other fungal beta-isopropylmalate dehydrogenases thus confirming the identity of the gene. The isolated ALEU2 gene was tested for its suitability to complement the auxotrophy of an A. adeninivorans aleu2 host. For this purpose the plasmid pAL-ALEU2m which contains the ALEU2 gene as a selection marker and the 25S rDNA for targeting was employed in transformation experiments. Transformants harboured a single copy of the heterologous DNA and were found to be mitotically stable. For assessment of heterologous gene expression, two model genes were incorporated into the vector: the GFP gene, encoding intracellular green fluorescent protein, and the HSA gene, encoding the secreted human serum albumin. For expression control, both gene sequences were fused to the constitutive A. adeninivorans-derived TEF1 promoter and the Saccharomyces cerevisiae-derived PHO5 terminator. In the respective recombinant strains the GFP was localised in the cytoplasm, whereas more than 95% of the HSA accumulated in the culture medium. In initial fermentation trials using a 200-ml shake flask, maximal HSA product levels were observed after 96 h of cultivation.

show abstract

Molecular cloning and sequence analysis of the Zygosaccharomyces rouxiiLEU2 gene encoding a β‐isopropylmalate dehydrogenase

Cited by 7 publications

References 22 publications

Ionic liquid aqueous solvent‐based microwave‐assisted hydrolysis for the extraction and HPLC determination of myricetin and quercetin from Myrica rubra leaves

Ionic liquid aqueous solvent‐based microwave‐assisted hydrolysis for the extraction and HPLC determination of myricetin and quercetin from Myrica rubra leaves

Cloning, sequencing and application of the LEU2 gene from the sour dough yeast Candida milleri

The gene ? a new component for an -based expression platform

Contact Info

Product

Resources

About