Molecular cloning, characterization, and expression profiling analysis of Cry toxin receptor genes from sugarcane shoot borer Chilo infuscatellus (Snellen)

Wang, Jinda; Zhang, Jiasong; Guo, Yan-Fang; Chen, Lifei; Wang, Falv; Huang, Mei-Ting; Gao, San‐Ji; Wang, Ran

doi:10.1016/j.pestbp.2019.03.023

Cited by 6 publications

(8 citation statements)

References 56 publications

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“…Both APN1 (zinc‐binding metalloprotease/peptidase enzyme) and ALP are important Cry toxin receptors that are tethered to the membrane of gut epithelial cells via GPI anchors (Wang et al, 2019). Expectedly, we also predict GPI anchor sites towards the C‐terminal end in APN1 and ALP protein of G. mellonella .…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization and functional analysis of Cry toxin receptor‐like genes from the model insect Galleria mellonella

Rao

Veeresh

Phani

et al. 2022

Insect Molecular Biology

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Crystal (Cry) toxins produced from the soil bacterium, Bacillus thuringiensis (Bt), have gained worldwide attention for long due to their insecticidal potential. A number of receptor proteins located on the epithelial cells of the larval midgut were shown to be crucial for Cry intoxication in different insect pests belonging to order Lepidoptera, Diptera and Coleoptera. A beehive pest, Galleria mellonella, serves as an excellent insect model for biochemical research. However, information on the Cry receptor‐like genes in G. mellonella is limited. In the present study, the full‐length sequences of four putative Cry receptor genes (ABC transporter, alkaline phosphatase, aminopeptidase N and cadherin) were cloned from G. mellonella. All these receptor genes were substantially upregulated in the midgut tissue of fourth‐instar G. mellonella larvae upon early exposure (6 h) to a sub‐lethal dose of Cry1AcF toxin. Oral and independent delivery of bacterially‐expressed dsRNAs corresponding to four receptor genes in G. mellonella suppressed the transcription of target receptors which in turn significantly reduced the larval sensitivity to Cry1AcF toxin. As the laboratory populations of G. mellonella develop Bt resistance in a relatively short time, molecular characterization of Cry receptor genes in G. mellonella performed in the present study may provide some useful information for future research related to the genetic basis of Bt resistance in the model insect.

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Section: Discussionmentioning

confidence: 99%

Molecular characterization and functional analysis of Cry toxin receptor‐like genes from the model insect Galleria mellonella

Rao

Veeresh

Phani

et al. 2022

Insect Molecular Biology

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“…31 There have been several attempts to control these pests, including the use of chemical pesticides and biological control. [32][33][34] Beauveria bassiana, a broad spectrum pest-killing microorganism, has been used to explore sustainable and environmentally friendly strategies to control borers, and C. infuscatellus was found to be infected more quickly than another sugarcane borer, Chilo venosatum. 35 In addition, two types of sex pheromones, Z11-16:OH and 16:OH have been identified from female moths of C. infuscatellus by gas chromatography linked to mass spectrometry (GC-MS).…”

Section: Introductionmentioning

confidence: 99%

“…In northern China, early instars of C. infuscatellus larvae can get into plant stalks and cause serious harm to the millet, corn, sorghum and other crops, and have caused severe damage to some crops yield in recent years 31 . There have been several attempts to control these pests, including the use of chemical pesticides and biological control 32–34 . Beauveria bassiana , a broad spectrum pest‐killing microorganism, has been used to explore sustainable and environmentally friendly strategies to control borers, and C. infuscatellus was found to be infected more quickly than another sugarcane borer, Chilo venosatum 35 .…”

Section: Introductionmentioning

confidence: 99%

Odorant‐binding proteins involved in sex pheromone and host‐plant recognition of the sugarcane borer Chilo infuscatellus (Lepidoptera: Crambidae)

Liu

Hu²,

et al. 2020

Pest Management Science

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BACKGROUND: Pheromone-binding proteins (PBPs) are responsible for transporting sex pheromones and general odorantbinding proteins (GOBPs) have been proposed to transport host-plant volatiles. A large number of OBPs have been identified from Lepidoptera species. However, olfactory molecular biology and physiology studies on PBP and GOBP in sugarcane pests are limited. Chilo infuscatellus is one of the most widely distributed pests in sugarcane-producing areas. RESULTS: Three PBPs (CinfPBP1, CinfPBP2 and CinfPBP3) and two GOBPs (CinfGOBP1 and CinfGOBP2) were identified, and five olfactory gene transcripts were abundantly expressed in antennae of C. infuscatellus. Binding assays showed that CinfPBP1-3 exhibited strong binding affinity for the sex pheromone components Z11-16:OH and 16:OH of C. infuscatellus. Meanwhile, Cinf-GOBP1-2 had high binding affinity with host-plant volatiles from sugarcane (Saccharum officinarum). Field-trapping results suggested that four volatile components, octadecane, (Z)-3-hexen-1-ol, ⊍-terpineol and hexadecane from host plants and sex pheromone mixed baits have synergistic roles in attracting C. infuscatellus adult moths. CONCLUSION: Functional characterization of CinfPBPs and CinfGOBPs in C. infuscatellus could help us find new environmentally friendly alternatives to conventional pest control using pesticides in sugarcane fields.

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“…Previous studies showed that Cry receptors in Chilo infuscatellus , including three ALPs, four APNs and a cadherin, were induced after applying Cry1Ac toxin (Wang et al ., 2019). In Cnaphalocrocis medinalis , four APNs and two ALPs were upregulated, while two APNs and a cadherin were downregulated after Cry1C treatment (Yang et al ., 2018).…”

Section: Discussionmentioning

confidence: 99%

Genome‐wide identification and comparative analysis of Cry toxin receptor families in 7 insect species with a focus on Spodoptera litura

Mengge

Zhu

et al. 2021

Insect Science

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Cadherin, aminopeptidase N (APN) and alkaline phosphatase (ALP) have been characterized as Cry receptors. In this study, comparative genomic analysis of the 3 receptor families was performed in 7 insects. ALPs and APNs are divided into three and eight clades in phylogenetic trees, respectively. ALPs in clade 3 and APNs in clade 1 contain multiple paralogs within each species and most paralogs are located closely in chromosomes. Drosophila melanogaster has expanded APNs in clade 5 and were lowly expressed in midgut. Cadherins are divided into 16 clades; they may diverge before holometabolous insect speciation except for BtR and Cad89D‐like clades. Eight insects from different orders containing BtR orthologs are sensitive to Cry1A or Cry3A, while five species without BtR are insensitive to both toxins. Most APNs in clade 1, several ALPs in clade 3, BtR and Cad89D‐like genes were highly or moderately expressed in larval midgut of Spodoptera litura and the other six species, and several members in these clades have been identified as Cry receptors. Expressions of putative S. litura Cry receptors in the midgut after exposing to Bt toxins were also analyzed.

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Molecular cloning, characterization, and expression profiling analysis of Cry toxin receptor genes from sugarcane shoot borer Chilo infuscatellus (Snellen)

Cited by 6 publications

References 56 publications

Molecular characterization and functional analysis of Cry toxin receptor‐like genes from the model insect Galleria mellonella

Molecular characterization and functional analysis of Cry toxin receptor‐like genes from the model insect Galleria mellonella

Odorant‐binding proteins involved in sex pheromone and host‐plant recognition of the sugarcane borer Chilo infuscatellus (Lepidoptera: Crambidae)

Genome‐wide identification and comparative analysis of Cry toxin receptor families in 7 insect species with a focus on Spodoptera litura

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