Molecular cloning, expression analysis and functional characterization of interleukin-22 in So-iny mullet, Liza haematocheila

The effects of five dietary lipid sources on growth, body composition and antioxidant parameters of the clamworm,Perinereis aibuhitensis

“…The gene-specific primers used in this study are shown in Table 2. Extraction of total RNA, synthesis of cDNA and real-time PCR were performed according to Qi et al (2015). For each dietary treatment, four clamworms were used, and the measurements were performed using four replicates.…”

Transcriptome analysis of soiny mullet (Liza haematocheila) spleen in response to Streptococcus dysgalactiae

“…Fish were acclimated for at least 7 days and checked randomly to confirm that no bacterial infection occurred in liver, spleen and blood. For the S. dysgalactiae challenge, twenty health soiny mullet were injected intraperioneally (i.p) with 100 mL live bacterial (2 Â 10 6 CFU) [2]. The fish in PBS-mock challenged group were injected with the same amount of PBS.…”

“…Our previous studies had revealed that S. dysgalactiae was one of bacterial pathogen which caused high mortalities to soiny mullet and induced immune genes expression in several tissues including spleen [2,30]. To gain further information on the molecular response of soiny mullet during bacterial infection, the differentially expressed unigenes were analyzed in detail.…”

“…In addition to its commercial value, the soiny mullet is also an ideal experimental animal model for studying environmental biology and ecology [1]. However, the increasing occurrence of pathogenic diseases, especially the bacterial diseases, in soiny mullet under intensive culture conditions has greatly hampered the production of this species [2]. Vaccine development against specific pathogens and molecular breeding strains of fish with enhanced resistance to major diseases are effective and environmental friendly [3,4].…”

“…Therefore, to gain deeper insight to the immunogenetics and molecular immune mechanisms underlying the response to bacterial infection of the soiny mullet, de novo transcriptome sequencing of spleen tissue of soiny mullet infected with Streptococcus dysgalactiae, a kind of gram positive bacterial [2], was performed using Illumina HiSeq2000 sequencing platform, and performed a global survey of immune-related genes, annotation of immune signaling pathways and determination of gene expression. Furthermore, putative simple sequence repeats (SSRs) and single nucleotide polymorphisms (SNPs) were analyzed.…”