Transcriptome analysis of soiny mullet (Liza haematocheila) spleen in response to Streptococcus dysgalactiae

Qi, Zhitao; Wu, Ping; Zhang, Qihuan; Wei, Youchuan; Wang, Zisheng; Qiu, Ming; Shao, Rong; Li, Yao; Gao, Qian

doi:10.1016/j.fsi.2015.12.029

Cited by 48 publications

(17 citation statements)

References 44 publications

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“…These results suggest that these immune pathways contribute to host defence against GCSD infection. However, T‐cell‐related genes were downregulated in the head kidney and spleen after the infection, contrary to the transcriptomic profile of the soiny mullet (Maekawa et al., 2019b; Qi, Wu, et al., 2016). From further analysis, we found the overexpression of arginase 2 gene (ARG2) in infected cobia, which catalyses the conversion of L‐arginine (for T‐cell proliferation) to L‐ornithine and urea.…”

Section: Host Immune Defencesmentioning

confidence: 79%

“…In soiny mullet, several immune regulators, suppressor of cytokine signalling (SOCSs), β‐defensin and interleukin‐22 (IL‐22) have been identified as responses to the GCSD infection (Qi, Xu, et al., 2016; Qi et al., 2015; Song et al., 2019). The transcriptomic profile of the spleen from soiny mullet infected with GCSD has been reported using the Illumina‐based paired‐end sequencing (Qi, Wu, et al., 2016). Following GCSD infection, 11,461 differentially expressed unigenes (4,658 upregulated and 6,803 downregulated unigenes) were identified, compared with the control fish.…”

Section: Host Immune Defencesmentioning

confidence: 99%

“…Following GCSD infection, 11,461 differentially expressed unigenes (4,658 upregulated and 6,803 downregulated unigenes) were identified, compared with the control fish. These differentially expressed unigenes were significantly enriched in immune‐related pathways, including the B‐cell receptor signalling pathway, complement and coagulation cascades, T‐cell receptor signalling pathway and Toll‐like receptor (Qi, Wu, et al., 2016).…”

Section: Host Immune Defencesmentioning

confidence: 99%

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Group C Streptococcus dysgalactiae infection in fish

Maekawa

Wang

Yoshida

et al. 2020

Journal of Fish Diseases

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Streptococcus dysgalactiae subsp. dysgalactiae (GCSD) is a Gram‐positive, facultative anaerobic bacterium and mostly non‐β‐haemolytic with Lancefield group C antigen. GCSD infection has been identified in various vertebrates. From 2002 to the present, GCSD infection of fish has been reported to cause severe economic losses in aquaculture farms around the world. Moreover, GCSD isolates from teleosts have been identified in patients with ascending upper limb cellulitis. Therefore, the economic and clinical significance of GCSD has increased in aquaculture, livestock and human health. Many studies have been presented, from the first report of isolated GCSD in fish, to the pathogenesis, characterization, immune responses and vaccine development. In this review, we present the current knowledge of GCSD in teleosts.

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Section: Host Immune Defencesmentioning

confidence: 79%

Section: Host Immune Defencesmentioning

confidence: 99%

Section: Host Immune Defencesmentioning

confidence: 99%

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Group C Streptococcus dysgalactiae infection in fish

Maekawa

Wang

Yoshida

et al. 2020

Journal of Fish Diseases

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“…And so far, there were less studies on the mechanism of the fish AMPs. Thus, the present study described the successful cloning and characterization of β-defensin from soiny mullet ( Liza haematocheila ), an economically important aquaculture mugilid species in China and other Asian countries [ 17 ]. Since it is very difficult to determine experimentally the structure of Lhβ-defensin, computational method was adopted to probe into monomeric and dimeric structure of Lhβ-defensin by using protein docking and followed by molecular dynamics (MD) stimulation.…”

Section: Introductionmentioning

confidence: 99%

Cloning and Expression of β-Defensin from Soiny Mullet (Liza haematocheila), with Insights of its Antibacterial Mechanism

Meng

et al. 2016

PLoS ONE

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Beta-defensins are important part of innate immunity of fish, which are the first defense line against invading pathogens. In this study, the β-defensin (Lhβ-defensin) gene was cloned from spleen tissue of soiny mullet (Liza haematocheila). Lhβ-defensin cDNA was 747 bp in length, encoding 63 amino acids. Sequence alignment revealed that Lhβ-defensin contained six conserved cysteine residues and shared 97.5% sequence identities with grouper (Epinephelus coioides) β-defensin. Realtime PCR revealed that Lhβ-defensin was highest expressed in the immune related organs, such as spleen, kidney and gut of healthy fish. Following Streptococcus dysgalactiae infection, Lhβ-defensin was up-regulated in immune related organs, e.g. 17.6-fold in spleen and 10.87-fold in gut at 24 h post infection (hpi). Lhβ-defensin possessed a monomeric structure of a three-stranded anti-parallel β-sheet and an α-helix stabilized by three disulfide bonds formed by Cys30-Cys58, Cys36-Cys52, and Cys40-Cys59. In addition to the experimental work, computer simulation was also carried out to determine the possible conformation of β-defensin and its interaction with palmitoyloleoylphosphatidylglycerol (POPG), a model of bacteria membrane. The Lhβ-defensin was found to form dimeric structure stabilized by the van der Waals contacts of Leu35 and Cys37 in two anti-parallel β1-strands and the cation-π interaction between Tyr32 and Arg54 respectively in the two β1-strands. The most important interactions between β-defensin and membrane are the electrostatic interactions between Arg residues in β-defensin and head group of POPG bilayer as well as hydrogen bond interactions between them. Our results were useful for further understanding the potential mechanism of antimicrobial property of fish β-defensins.

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“…Information on host-bacteria interactions and the immune responses underlying E. fuscoguttatus and vibriosis are still largely unknown. Transcriptomic profiling analysis has been extensively applied in fish studies for the discovery of many immune-related genes and is a very useful technique in revealing the genetic response of host towards pathogen and disease infections (Ali et al 2014;Huang et al 2011;Qi et al 2016;Wang et al 2016). Thus, to achieve an overall success of gene expression analyses, a reliable and an efficient RNA isolation method is the first and most important step to obtain high quality RNA for gene expression studies (Bharudin et al 2014).…”

mentioning

confidence: 99%

Alternative RNA Extraction Method in Vibrio vulnificus Infected Brown-Marbled Grouper, Epinephelus fuscoguttatus

Jazamuddin

Aizat

Goh

et al. 2019

TLSR

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Abstrak: Vibriosis adalah penyakit akuatik berleluasa yang disebabkan oleh spesies Vibrio dan telah menyebabkan kerugian yang besar terhadap spesis akuatik terutamanya kerapu harimau, Epinephelus fuscoguttatus. Kerumitan mekanisme molekul yang berkaitan dengan pertahanan imun ikan dapat dikaji melalui pendekatan seperti analisis transkriptomik. Kualiti dan kuantiti jumlah RNA yang tinggi adalah penting dalam ketepatan penjujukan RNA dan analisis pengekspresan gen. Kualiti RNA yang rendah akan menjejaskan analisis dan mengakibatkan kerugian masa dan wang sekiranya data perlu dianalisa semula. Oleh itu, kaedah pengekstrakan RNA yang andal dan cekap adalah langkap pertama dan paling penting untuk mendapatkan RNA jumlah yang berkualiti tinggi terutamanya bagi kajian pengekspresan gen. Terdapat banyak aspek yang perlu diambil kira apabila memilih kaedah pengekstrakan seperti keberkesanan protokol tersebut, tempoh pendedahan kimia, tempoh masa yang diambil untuk pengekstrakan dan bilangan pemindahan sampel. Protokol pengekstrakan RNA yang baik juga perlu dapat menghasilkan jumlah dan ketulenan RNA yang tinggi dan bebas daripada perencat enzim seperti nuklease (RNase), fenol, alkohol, sisa bahan kimia, protein, dan genomik DNA bagi memastikan keutuhan RNA yang diekstrak sentiasa dalam keadaan baik. Dalam kajian ini, TransZol TM Up Plus menghasilkan sampel RNA yang bersih dan tulen daripada tisu insang kawalan sahaja tetapi bukan daripada tisu insang yang dijangkiti dan juga tisu seluruh badan ikan. Kaedah konvensional CTAB (conventional hexadecyltrimethylammonium bromide) yang diubah suai kemudiannya digunakan sebagai kaedah alternatif untuk mengasingkan RNA dari insang dan tisu badan E. fuscoguttatus yang dijangkiti Vibrio. Kaedah CTAB telah menghasilkan jalur RNA yang utuh pada elektroforesis gel dengan nilai RIN yang lebih tinggi (>6.5), maka kaedah ini juga sesuai dalam pengekstrakan RNA jumlah yang berkualiti tinggi daripada sampel E. fuscoguttatus. Oleh itu kaedah ini berpotensi untuk digunapakai dalam pengestrakan RNA daripada spesis ikan terutamanya yang telah dijangkiti penyakit.

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Transcriptome analysis of soiny mullet (Liza haematocheila) spleen in response to Streptococcus dysgalactiae

Cited by 48 publications

References 44 publications

Group C Streptococcus dysgalactiae infection in fish

Group C Streptococcus dysgalactiae infection in fish

Cloning and Expression of β-Defensin from Soiny Mullet (Liza haematocheila), with Insights of its Antibacterial Mechanism

Alternative RNA Extraction Method in Vibrio vulnificus Infected Brown-Marbled Grouper, Epinephelus fuscoguttatus

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