Molecular cloning of a cDNA encoding molt-inhibiting hormone of the crab, Cancer magister

Cloning of Precursors for Two MIH/VIH-Related Peptides in the Prawn, Macrobrachium rosenbergii

“…Likewise, Northern blot analyses suggested higher expression of Mar-SGP-B than Mar-SGP-A mRNA in the eyestalk ganglia since Mar-SGP-A mRNA levels were below detection limits. The results of the present and previous studies (27,30,32,34,36) indicate that MIH and VIH mRNAs (Type II peptide) are transcribed in the eyestalk ganglia only, whereas CHH mRNA (Type I peptide) has multiple expression sites.…”

“…Less amino acid sequence identity was found near the C-terminal region, suggesting that the species-specific and activity-specific amino acid sequences are in this region. In previous reports, the transcripts of two MIHs from P. japonicus (Pej-SGP-IV), Cancer magister (Cam*-MIH), and VIH from H. americanus (Hoa-VIH) were detected only in eyestalk ganglia but not in other neuronal and nonneuronal tissues (30,32,34). Recently, a CHH from Metapenaeus ensis (Me-CHH-B) was detected in shrimp neuronal tissues (brain, thoracic ganglion and nerve cord, 27).…”

Members of the crustacean hyperglycemic hormone (CHH) peptide family are differentially distributed both between and within the neuroendocrine organs ofCancercrabs: implications for differential release and pleiotropic function

“…The preprohormones of the CHH subgroup always contain a second peptide, commonly referred to as CHH precursor-related peptide or CPRP, which is Cterminally flanked by the CHH (Weidemann et al, 1989;Tensen et al, 1991;de Kleijn et al, 1994a;Ohira et al, 1997a;Davey et al, 2000;Toullec et al, 2002;Marco et al, 2003;Chen et al, 2004;Mettulio et al, 2004;de la Iglesia et al, 2005;Hsu et al, 2005a;Toullec et al, 2006). The preprohormones of the MIH/GIH/VIH/MOIH sub-group lack precursor-related peptides (Klein et al, 1993;de Kleijn et al, 1994b;Sun, 1994;Lee et al, 1995;Ohira et al, 1997b;Chan et al, 1998;Gu and Chan, 1998;Umphrey et al, 1998;Tang et al, 1999;Lu et al, 2001;Yang and Rao, 2001;Edomi et al, 2002;Krungkasem et al, 2002;Ohira et al, 2005). Although CPRP has been shown to circulate in the hemolymph, the physiological role(s) it plays in crustaceans remains unknown (Wilcockson et al, 2002).…”

“…In this genus, four distinct CHH isoforms have been identified [two from Cancer pagurus and two from Cancer productus (Chung et al, 1998;de la Iglesia et al, 2005;Hsu et al, 2005a); Table·1] as have two isoforms of MIH [one from Cancer magister and the other from C. pagurus Umphrey et al, 1998); Table·1] and two isoforms of MOIH [both from C. pagurus (Wainwright et al, 1996); Table 1]. Similarly, nine isoforms of CPRP have been characterized from the SG of Cancer species [one from C. pagurus and four each from C. productus and Cancer borealis (Chung et al, 1998;de la Iglesia et al, 2005;Fu et al, 2005a;Fu et al, 2005b;Hsu et al, 2005a); Table 1].…”

“…Cancer pagurus CHH I and II [Capa-CHH I and II (Chung et al, 1998)], Cancer productus CHH I and II [Capr-CHH I and II ], C. pagurus MIH [Capa-MIH ], Cancer magister MIH [Cama-MIH (Umphrey et al, 1998)], C. pagurus MOIH I I and II (Wainwright et al, 1996)], C. pagurus CPRP [Capa-CPRP (Chung et al, 1998)], C. productus I to IV [Capr-CPRP I to IV (Fu et al, 2005a)] and Cancer borealis [Cabo-CPRP I to IV (Fu et al, In each group of aligned sequences, amino acid residues identical to that used to generate the antibodies, indicated with an asterisk, are . To obtain the XO-SG system, the carapace encasing an eyestalk was split both dorsally and ventrally and one half of the split shell was gently teased away from the other half.…”