Molecular Cloning of a Gene Encoding Endo-β-<scp>D</scp>-1,4-Glucanase PCE1 from<i>Phycomyces nitens</i>

Shimonaka, Atsushi; Baba, Yuko; Koga, Jinichiro; Nakane, Akitaka; Kubota, Hitomi; Kono, Toshiaki

doi:10.1271/bbb.68.2299

Cited by 12 publications

(10 citation statements)

References 24 publications

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“…[9][10][11][12] In the present study, we compared the characteristics of various family 45 endoglucanases, and examined the specific common characteristics of family 45 endoglucanases from Mucorales in the use of textiles and laundry.…”

Section: )mentioning

confidence: 99%

“…8) Furthermore, Zygomycetes is classified into 12 orders: Mucorales, Entomophthorales, Zoopagales, Endogonales, Amoebidiales, Eccrinales, Harpellales, Asellariales, etc. In our previous studies, six endoglucanases, RCE1, RCE2, and RCE3 from Rhizopus oryzae, 9,10) MCE1 and MCE2 from Mucor circinelloides, 11) and PCE1 from Phycomyces nitens 12) were isolated as family 45 endoglucanases from Mucorales, and the encoding genes rce1, rce2, rce3, mce1, mce2, and pce1 were cloned. Of various fungal family 45 endoglucanases, only RCE1, RCE2, RCE3, MCE1, MCE2, and PCE1 had cellulose-binding module family 1 (CBM1) at the N terminus, structures unique to family 45 endoglucanases from Mucorales.…”

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“…Of various fungal family 45 endoglucanases, only RCE1, RCE2, RCE3, MCE1, MCE2, and PCE1 had cellulose-binding module family 1 (CBM1) at the N terminus, structures unique to family 45 endoglucanases from Mucorales. [10][11][12] The optimum temperatures for carboxymethyl cellulase (CMCase) activities of RCE1, RCE2, MCE1, MCE2, and PCE1 were 45 to 55 C, lower than those of the other family 45 endoglucanases (55 to 75 C). [9][10][11][12] In the present study, we compared the characteristics of various family 45 endoglucanases, and examined the specific common characteristics of family 45 endoglucanases from Mucorales in the use of textiles and laundry.…”

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Specific Characteristics of Family 45 Endoglucanases from Mucorales in the Use of Textiles and Laundry

Shimonaka¹,

Koga²,

Baba³

et al. 2006

Bioscience, Biotechnology, and Biochemistry

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We examined the characteristics of family 45 endoglucanases (glycoside hydrolases family 45; GH45) from Mucorales belonging to Zygomycota in the use of textiles and laundry. The defibrillation activities on lyocell fabric of family 45 endoglucanases from Mucorales, such as RCE1 and RCE2 from Rhizopus oryzae, MCE1 and MCE2 from Mucor circinelloides, and PCE1 from Phycomyces nitens, were much higher than those of the other family 45 endoglucanases. By contrast, family 45 endoglucanases from Mucorales were less resistant to anionic surfactant and oxidizing agent, main components in detergents, than the other family 45 endoglucanases. RCE1 consists of two distinct modules, a catalytic module and a carbohydrate-binding module family 1 (CBM1), and these common specific characteristics were considered to due to the catalytic module, but not to the CBM1.Key words: endoglucanase; cellulase; glycoside hydrolase family 45; Zygomycota; Mucorales Cellulose is the major plant cell wall polysaccharide and is degraded by cellulases. This degradation is considered to be achieved by the synergistic action of three types of cellulase components: endoglucanases (EC 3.2.1.4, endo--D-1,4-glucanases), cellobiohydrolases (EC 3.2.1.91), and -glucosidases (EC 3.2.1.21). 1)Most cellulosic fabrics consisting of cellulosic fibers, such as cotton, linen, ramie, viscose, and lyocell, have a tendency to form microfibrils. The accumulation of microfibrils on the surface of the fabrics make the fabrics look hairy and scatters incident light, thereby lessening the brightness of the original colors. These phenomena are considered to be negative features of cellulosic fabrics. Hence, the removal of the microfibrils (defibrillation) is necessary to increase the commercial value of cellulosic fabrics. In the textile and detergent industries, endoglucanases are used to remove microfibrils from the surface of cellulosic fabrics, enhancing color brightness. [2][3][4][5] Although lyocell fabrics possess many positive attributes for high-quality fabrics, they have the negative characteristic to form larger quantities of microfibrils than other cellulosic fabrics in the dyeing, finishing, and washing processes. Hence, high defibrillation of lyocell fabrics by endoglucanase treatment is in demand for textile use. 6)Family 45 endoglucanases (glycoside hydrolases family 45; GH45) are the main endoglucanases used in the textile and detergent industries because of their high defibrillation activities, 6,7) and many fungal family 45 endoglucanases have been purified and characterized. The fungal kingdom is classified into four phylums: Ascomycota, Basidiomycota, Chytridiomycota, and Zygomycota. Zygomycota is classified into two classes: Zygomycetes and Trichomycetes.8) Furthermore, Zygomycetes is classified into 12 orders: Mucorales, Entomophthorales, Zoopagales, Endogonales, Amoebidiales, Eccrinales, Harpellales, Asellariales, etc. In our previous studies, six endoglucanases, RCE1, RCE2, and RCE3 from Rhizopus oryzae, 9,10) MCE1 and MCE2 from Mucor circinelloid...

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Specific Characteristics of Family 45 Endoglucanases from Mucorales in the Use of Textiles and Laundry

Shimonaka¹,

Koga²,

Baba³

et al. 2006

Bioscience, Biotechnology, and Biochemistry

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“…The I 50 value was measured as the concentration of LAS or sodium hypochlorite that inhibited 50% of the maximum CMCase activity. The resistance of BCE1 to anionic surfactant and oxidizing agent was compared with those of the other family 45 endoglucanases, RCE1 and RCE2 from Rhizopus oryzae, 11,12) PCE1 from Phycomyces nitens, 13) and EGL3 and EGL4 from Humicola grisea.…”

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confidence: 99%

“…RCE1, 11) RCE2, 11) PCE1, 13) EGL3, 14,15) and EGL4 14,16) were purified as described previously. Since one of the more prevalent anionic surfactants in industrial use is LAS, we examined the resistance of each endoglucanase to LAS.…”

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Purification and Characterization of a New Endo-1,4-β-D-glucanase fromBeltraniella portoricensis

Baba¹,

Shimonaka²,

Koga³

et al. 2005

Bioscience, Biotechnology, and Biochemistry

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Mucoromycota fungi as powerful cell factories for modern biorefinery

Dzurendová

Losada

Dupuy--Galet

et al. 2021

Appl Microbiol Biotechnol

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Biorefinery employing fungi can be a strategy for valorizing low-cost rest materials, by-products and wastes into several valuable bioproducts through the fungal fermentation. Mucoromycota fungi are soil fungi with a highly versatile metabolic system that positions them as powerful microbial cell factories for biorefinery applications. Lipids, pigments, chitin/chitosan, polyphosphates, ethanol, organic acids and enzymes are main Mucoromycota products that can be refined from the fermentation process and applied in nutrition, chemical or biofuel industries. In addition, Mucoromycota biomass can be used as it is for specific purposes, such as feed. Mucoromycota fungi can be employed in developing co-production processes, whereby several intra-and extracellular products are simultaneously formed in a single fermentation process, and, thus, economic viability of the process can be improved. This mini review provides a comprehensive overview over the recent advances in the production of valuable metabolites by Mucoromycota fungi and fermentation strategies which could be potentially applied in the industrial biorefinery settings. Key points• Biorefineries utilizing Mucoromycota fungi as production cell factories can provide a wide range of bioproducts.• Mucoromycota fungi are able to perform co-production of various metabolites in a single fermentation process.• Versatile metabolism of Mucoromycota allows valorization of a various low-cost substrates such as wastes and rest materials.

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Molecular Cloning of a Gene Encoding Endo-β-D-1,4-Glucanase PCE1 fromPhycomyces nitens

Cited by 12 publications

References 24 publications

Specific Characteristics of Family 45 Endoglucanases from Mucorales in the Use of Textiles and Laundry

Specific Characteristics of Family 45 Endoglucanases from Mucorales in the Use of Textiles and Laundry

Purification and Characterization of a New Endo-1,4-β-D-glucanase fromBeltraniella portoricensis

Mucoromycota fungi as powerful cell factories for modern biorefinery

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