2004
DOI: 10.1007/s00425-004-1428-x
|View full text |Cite
|
Sign up to set email alerts
|

Molecular cloning of a pathogen/wound-inducible PR10 promoter from Pinus monticola and characterization in transgenic Arabidopsis plants

Abstract: In Pinus monticola (Dougl. ex D. Don), the class ten pathogenesis-related (PR10) proteins comprise a family of multiple members differentially expressed upon pathogen infection and other environmental stresses. One of them, PmPR10-1.13, is studied here by investigating its transcriptional regulation in transgenic Arabidopsis plants. For functional analyses of the PmPR10-1.13 promoter, a 1,316-bp promoter fragment and three 5' deletions were translationally fused to the ss-glucuronidase (GUS) reporter gene. The… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

2
32
0

Year Published

2007
2007
2021
2021

Publication Types

Select...
9
1

Relationship

1
9

Authors

Journals

citations
Cited by 54 publications
(34 citation statements)
references
References 65 publications
2
32
0
Order By: Relevance
“…Then, the promoter sequence was transferred into the binary vector pKGWFS7 (Karimi et al 2002) to express an Egfp-gus reporter gene. Arabidopsis transformation, plant treatments with wounding and infection by Pseudomonas syringae (strain Psm4326), and GUS activity analysis of transgenic plants were performed as described in a previous report (Liu et al 2005).…”
Section: Analysis Of Pmtnl1:: Gus Fusion Gene In Transgenic Arabidopsismentioning
confidence: 99%
“…Then, the promoter sequence was transferred into the binary vector pKGWFS7 (Karimi et al 2002) to express an Egfp-gus reporter gene. Arabidopsis transformation, plant treatments with wounding and infection by Pseudomonas syringae (strain Psm4326), and GUS activity analysis of transgenic plants were performed as described in a previous report (Liu et al 2005).…”
Section: Analysis Of Pmtnl1:: Gus Fusion Gene In Transgenic Arabidopsismentioning
confidence: 99%
“…Examples are Bet v 1 from birch (Swoboda et al, 1996), Mal d 1 from apple (Malus domestica; Vanek-Krebitz et al, 1995), Pru ar 1 from apricot (Prunus armeniaca), Pru av 1 from cherry (Neudecker et al, 2001), and Dau c 1 from carrot (Daucus carota; Yamamoto et al, 1997). On the other hand, several PR-10 proteins are up-regulated upon pathogen infection (Walter et al, 1990;Breda et al, 1996;Pü hringer et al, 2000;Park et al, 2004;Liu et al, 2005), have a direct and selective antifungal activity, or accumulate in overwintering organs of tree species, suggesting a key role in selective defense mechanisms against microbes and fungi and in protecting plants from abiotic stresses (Yu et al, 2000;Flores et al, 2002;Chadha and Das, 2006). In addition, a number of PR-10 proteins are constitutively expressed at different plant developmental stages and/ or in different tissues and organs, such as in seeds, roots (Sikorski et al, 1999), flowers, and senescent leaves (Breiteneder et al, 1989;Crowell et al, 1992;Barrat and Clark, 1993;Vanek-Krebitz et al, 1995;Sikorski et al, 1999), suggesting a role for PR-10 proteins also in developmental regulation.…”
mentioning
confidence: 99%
“…Characterization of PR-10 proteins and development of transgenic plants overexpressing PR-10 proteins is important step in this direction. Table 1 lists the PR-10 genes which have been used to develop transgenic plants in different crop species [95][96][97][98][99][100][101][102][103][104][105][106][107][108][109][110]. Multi-location field trials of transgenic plants expressing PR-10 will likely be next step for further evaluation.…”
Section: Pr-10 Proteins: a Resource For Crop Improvementmentioning
confidence: 99%