Molecular cloning of a potato leaf cDNA encoding an aspartic protease (StAsp) and its expression after P. infestans infection

Guevara, María Gabriela; Almeida, Carla; Mendieta, Julieta Renée; Faro, Carlos; Verı́ssimo, Paula; Pires, Euclides; Daleo, Gustavo Raúl

doi:10.1016/j.plaphy.2005.07.004

Cited by 43 publications

(47 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In agreement with previous reports showing that many plant APs contain one or more N-linked glycans, StAPs are glycosylated [14,15]. On the other hand, StAPs are induced by both abiotic and biotic stress [15,16], have extracellular localization and have antimicrobial activity in vitro towards Phytophthora infestans and Fusarium solani, two potato pathogens [16][17][18].…”

Section: Introductionsupporting

confidence: 91%

Roles of glycosylation on the antifungal activity and apoplast accumulation of StAPs (Solanum tuberosum aspartic proteases)

Pagano¹,

Mendieta²,

Muñoz³

et al. 2007

International Journal of Biological Macromolecules

Self Cite

View full text Add to dashboard Cite

Section: Introductionsupporting

confidence: 91%

Roles of glycosylation on the antifungal activity and apoplast accumulation of StAPs (Solanum tuberosum aspartic proteases)

Pagano¹,

Mendieta²,

Muñoz³

et al. 2007

International Journal of Biological Macromolecules

Self Cite

View full text Add to dashboard Cite

“…In resistant genotypes P. infestans infection triggers a systemic acquired resistance (SAR) response, where the expression of SAR genes correlates with local necrotic lesions [13]. In SAR signals are transported from the infection site to other parts of the plant.…”

mentioning

confidence: 99%

Expression of biotic stress response genes to Phytophthora infestans inoculation in White Lady, a potato cultivar with race-specific resistance to late blight

Hajianfar

Kolics

Cernák

et al. 2016

Physiological and Molecular Plant Pathology

View full text Add to dashboard Cite

a b s t r a c tExpression changes of biotic stress response genes were analyzed during a 65 h period post inoculation with Phytophthora infestans in potato cultivar White Lady that possesses race-specific resistance to this pathogen. All analyzed respiratory burst oxidase homologs, the PR proteins, the serine-, cysteine-and aspartic protease inhibitors, as well as the Rpi-bt1 gene homolog were up-regulated in the biotrophic phase. The R1 and R2 gene homologs showed up-regulation only at 65 hpi, and interestingly, the R3a gene showed only a very slight expressional increase. It is concluded, that beside the constitutively expressed R genes a number of non-specific stress response genes contribute to the successful resistance response in race-specific defense.

show abstract

“…In plants, the production of proteases is believed to be a general defense mechanism against pathogenic fungi. Secretion of aspartic proteases with antimicrobial activity against Phytophthora infestans and the conidia of Fusarium solani has been described in potato tubers (Solanum tuberosum) after infection and damage to the intercellular fluids (Guevara et al, 2001(Guevara et al, , 2002(Guevara et al, , 2005Mendieta et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

The inter-generic fungicidal activity of Xanthophyllomyces dendrorhous

et al. 2010

View full text Add to dashboard Cite

In this study, the existence of intra-specific and inter-generic fungicidal activity in Xanthophyllomyces dendrorhous and Phaffia rhodozyma strains isolated from different regions of the earth was examined. Assays were performed under several culture conditions, showing that all the analyzed X. dendrorhous and P. rhodozyma strains have killing activity against Kloeckera apiculata, Rhodotorula sloffiae, and R. minuta. This activity was greater in rich media at a pH from 4.6 to 5.0. Extracellular protein extracts with fungicidal activity were obtained from cultures of all strains, and their characterization suggested that a protein of 33 kDa is the antifungal factor. According to peptide mass fingerprinting and an analysis of the results with the MASCOT search engine, this protein was identified as an aspartic protease. Additionally, extrachromosomal double-stranded DNA elements (dsDNAs) were observed in all X. dendrorhous and P. rhodozyma strains. Although there is a high variability, two dsDNAs of 5.4 and 6.8 kb are present in all strains.

show abstract

Molecular cloning of a potato leaf cDNA encoding an aspartic protease (StAsp) and its expression after P. infestans infection

Cited by 43 publications

References 39 publications

Roles of glycosylation on the antifungal activity and apoplast accumulation of StAPs (Solanum tuberosum aspartic proteases)

Roles of glycosylation on the antifungal activity and apoplast accumulation of StAPs (Solanum tuberosum aspartic proteases)

Expression of biotic stress response genes to Phytophthora infestans inoculation in White Lady, a potato cultivar with race-specific resistance to late blight

The inter-generic fungicidal activity of Xanthophyllomyces dendrorhous

Contact Info

Product

Resources

About