Molecular cloning of cDNAs for 14-3-3 and its protein interactions in a white-rot fungusPhanerochaete chrysosporium

Abstract

The 14-3-3 is a highly conserved, ubiquitous expressed protein in eukaryotes and very important in the regulation of such crucial cellular processes as metabolism, signal transduction, cell-cycle control, apoptosis, protein trafficking, transcription, stress responses, and malignant transformation. In this study, the full-length cDNA of 14-3-3 was cloned first time from a white-rot fungus Phanerochaete chrysosporium. Yeast two hybrid has been performed to fish out the proteins that can bind with 14-3-3 in P. chrysosporium. The results showed that 14-3-3 could form homodimers in P. chrysosporium. Two novel proteins containing predicted WD domain could interact with 14-3-3, too. In addition, the transcription of 14-3-3 gene under low-nitrogen medium was constitutional by RT-PCR analysis. These results indicate that 14-3-3 protein in P. chrysosporium may be involved in multiple cellular processes.