Molecular cloning of leucocyte cell-derived chemotaxin-2 gene in croceine croaker (Pseudosciaena crocea)

Prokaryotic expression, purification, and refolding of leukocyte cell-derived chemotaxin 2 and its effect on gene expression of head kidney-derived macrophages of a teleost fish, ayu (Plecoglossus altivelis)

“…Recently, several reports in fish reveal that LECT2 transcripts remarkably increase after bacterial infection, indicating that this protein may participate in fish immune responses [8,9]. Most recently, an interaction between LECT2 and CLR of ayu was identified by using a yeast two-hybrid system and co-immunoprecipitation, and ayu CLR is found to be expressed at high levels in the head kidney and leukocytes [11].…”

“…Recently, studies on the immune response in some fish showed that LECT2 transcripts increased dramatically after bacterial infections, shedding new lights on the function of this gene in fish [8,9]. Most recently, we found for the first time that ayu LECT2 mature peptide (aLECT2m) could interact with the single extracellular C-type lectin domain of a C-type lectin receptor (CLR) [10], which is one of pattern recognition receptors (PRRs) present on fish immune cells [11].…”

Atlantic salmon (Salmo salar) liver transcriptome response to diets containing Camelina sativa products

“…Both lect-2 and chitinase 3 are involved in inflammation (Kawada et al, 2007;Li et al, 2008). Lect-2 acts as a chemotactic factor to activate neutrophils, whose transcript expression was shown to be induced in the liver and spleen of Vibrio alginolyticus-infected croceine croaker (Pseudosciaena crocea) .…”

A new leukocyte cell-derived chemotaxin-2 from marine fish grouper, Epinephelus coioides: Molecular cloning and expression analysis

“…The expression levels of EcLECT2 were up-regulated by challenging with S. aureus, V. vulnificus, V. parahaemolyticus and S. cerevisiae in liver. The expression of other LECT2s such as pLECT2 from croceine croaker (P. crocea) and LECT2 from zebrafish liver also were up-regulated by bacteria challenge [10,11]. The expression levels of pLECT2 in V. alginolyticus-infected fish were significantly increased in liver (up to 80.82-fold) and spleen (up to 58.56-fold) comparing to those in healthy fish.…”

“…Proteins with sequence similarity to LECT2 have been identified in many (fresh-water) fishes such as carp (Cyprinus carpio) [8], rainbow trout (Oncorhynchus mykiss) [9], croceine croaker (Pseudosciaena crocea) [10] and zebrafish (Danio rerio) [11], but has not been reported in marine fishes such as grouper.…”