Molecular Cloning of Microtubule‐Associated Protein 1 (MAP1A) and Microtubule‐Associated Protein 5 (MAP1B): Identification of Distinct Genes and Their Differential Expression in Developing Brain

Garner, Craig C.; Garner, Abigail M.; Huber, G.; Kozak, Christine A.; Matus, Andrew

doi:10.1111/j.1471-4159.1990.tb08832.x

Cited by 129 publications

(76 citation statements)

References 55 publications

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“…These included cytoskeleton proteins such as septin 2, 4, 7, and 8; MTAP1a and 1b; and ROCK. MTAP and septins are known components of myelin (47)(48)(49). As myelin wraps multiple times around the axon, the formation and maintenance of the myelin sheath is dependent on the cytoskeleton, probably explaining the abundance of cytoskeletal proteins in myelin preparations (50).…”

Section: Discussionmentioning

confidence: 99%

Identification of the Low Density Lipoprotein (LDL) Receptor-related Protein-1 Interactome in Central Nervous System Myelin Suggests a Role in the Clearance of Necrotic Cell Debris

Fernández-Castañeda

Arandjelovic

Stiles

et al. 2013

Journal of Biological Chemistry

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Background: LRP1 is a scavenger receptor involved in the clearance of apoptotic cells and myelin vesicles. Results: Novel ligands for LRP1 were discovered in CNS myelin by affinity purification combined with proteomics. Conclusion: Some ligands are intracellular proteins, suggesting a function for LRP1 in the clearance of necrotic debris. Significance: LRP1 mediates the removal of cellular waste and could maintain homeostasis.

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Section: Discussionmentioning

confidence: 99%

Identification of the Low Density Lipoprotein (LDL) Receptor-related Protein-1 Interactome in Central Nervous System Myelin Suggests a Role in the Clearance of Necrotic Cell Debris

Fernández-Castañeda

Arandjelovic

Stiles

et al. 2013

Journal of Biological Chemistry

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“…The two proteins are reciprocally expressed over the lifespan of an organism, with MAP1B being expressed earlier during development, at a time when the cytoskeleton is quite dynamic. MAP1A typically is expressed later when MAP1B is down-regulated and is believed to confer stability to extended neurites (35)(36)(37)(38)(39)(40)(41).…”

Section: Discussionmentioning

confidence: 99%

Mapmodulin/Leucine-rich Acidic Nuclear Protein Binds the Light Chain of Microtubule-associated Protein 1B and Modulates Neuritogenesis

Opal

Garcı́a

Propst

et al. 2003

Journal of Biological Chemistry

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We had previously described the leucine-rich acidic nuclear protein (LANP) as a candidate mediator of toxicity in the polyglutamine disease, spinocerebellar ataxia type 1 (SCA1). This was based on the observation that LANP binds ataxin-1, the protein involved in this disease, in a glutamine repeat-dependent manner. Furthermore, LANP is expressed abundantly in purkinje cells, the primary site of ataxin-1 pathology. Here we focused our efforts on understanding the neuronal properties of LANP. In undifferentiated neuronal cells LANP is predominantly a nuclear protein, requiring a bona fide nuclear localization signal to be imported into the nucleus. LANP translocates from the nucleus to the cytoplasm during the process of neuritogenesis, interacts with the light chain of the microtubule-associated protein 1B (MAP1B), and modulates the effects of MAP1B on neurite extension. LANP thus could play a key role in neuronal development and/or neurodegeneration by its interactions with microtubule associated proteins. Spinocerebellar ataxia type 1 (SCA1) 1 belongs to a group of disorders in which a polyglutamine expansion in the disease protein launches a cascade of events that causes relentless neurodegeneration. We had previously proposed that the leucine-rich acidic nuclear protein (LANP) stands out as a particularly appealing candidate mediator of toxicity in SCA1 based on its ability to interact with ataxin-1 in a glutamine repeat-dependent manner (1). Moreover, LANP is expressed at particularly high levels in purkinje cells, the seat of SCA1 pathology. Thus, one could envisage a scenario where the functions of LANP could be altered upon binding to ataxin-1, triggering downstream toxic events. This could also account for the regional toxicity of ataxin-1, despite its own ubiquitous expression.Since its first description in 1994, LANP has been implicated in myriad cellular functions from the cell surface to the nucleus. First described as a putative human leukocyte antigen class II-associated protein (and hence called PHAPI), it was suspected to be involved in signal transduction in lymphocytes (2). Matsuoka et al. (1994) independently described this protein in the developing cerebellum, and noting that it contained a leucine-rich repeat, called it by the acronym LANP. With a modular architecture reminiscent of a tadpole, LANP consists of a globular head formed by the N-terminal leucine-rich domain containing five leucine-rich repeats (LRR) and a C-terminal tail formed by the remaining length of acidic residues (3). As such, it belongs to a large and very interesting family of proteins that contain LRRs crucial for protein interactions, by forming a very characteristic secondary structure designed for protein-protein interactions (4 -6). It was therefore proposed to be a modulator of signaling pathways in cerebellar morphogenesis.LANP has since been implicated in a number of other functions: as a phosphorylated protein, LANP (known in this context as phosphoprotein 32 or pp32) was suggested to act as tumor suppressor (7...

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“…MAP1B (Bloom et al, 1995) is the first MAP that is specifically expressed during neural development and that is especially prominent in neurons that are actively extending axons (Calvert and Anderton, 1985;Garner et al, 1990;Fischer and Romano-Clarke, 1991;Ulloa et al, 1993;Black et al, 1994: DiTella et al, 1996Gordon-Weeks and Fischer, 2000). These findings led to the hypothesis that MAP1B might be involved in axon formation by regulating microtubule dynamics.…”

Section: Introductionmentioning

confidence: 95%

“…Current evidence favors the view that several of the distinctive properties of neuronal microtubules, such as increased stability and spatial differentiation, arise from the developmentally regulated expression of structural microtubule-associated proteins (MAPs), which are notably abundant in neurons (Maccioni and Cambiazo, 1995). Therefore, it is likely that the expression of these proteins along neuronal development may affect neuronal polarization.MAP1B (Bloom et al, 1995) is the first MAP that is specifically expressed during neural development and that is especially prominent in neurons that are actively extending axons (Calvert and Anderton, 1985;Garner et al, 1990;Fischer and Romano-Clarke, 1991;Ulloa et al, 1993;Black et al, 1994: DiTella et al, 1996Gordon-Weeks and Fischer, 2000). These findings led to the hypothesis that MAP1B might be involved in axon formation by regulating microtubule dynamics.…”

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confidence: 95%

Evidence for the Role of MAP1B in Axon Formation

González-Billault

Ávila

Cáceres

2001

MBoC

135

141

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Cultured neurons obtained from a hypomorphous MAP1B mutant mouse line display a selective and significant inhibition of axon formation that reflects a delay in axon outgrowth and a reduced rate of elongation. This phenomenon is paralleled by decreased microtubule formation and dynamics, which is dramatic at the distal axonal segment, as well as in growth cones, where the more recently assembled microtubule polymer normally predominates. These neurons also have aberrant growth cone formation and increased actin-based protrusive activity. Taken together, this study provides direct evidence showing that by promoting microtubule dynamics and regulating cytoskeletal organization MAP1B has a crucial role in axon formation. INTRODUCTIONNeurons are highly polarized cells that contain a single long axon and multiple dendrites. Polarization occurs when one of the multiple neurites emerging from the cell body initiates a phase of rapid elongation, becoming the axon; the remaining neurites will develop as dendrites Dotti, 1997, 1999;Dotti et al., 1998). Because microtubule assembly and stabilization play an essential role in axon formation (Mitchison and Kirschner, 1989) a great deal of attention has been devoted to identify factors controlling microtubule organization and dynamics in nerve cells. Current evidence favors the view that several of the distinctive properties of neuronal microtubules, such as increased stability and spatial differentiation, arise from the developmentally regulated expression of structural microtubule-associated proteins (MAPs), which are notably abundant in neurons (Maccioni and Cambiazo, 1995). Therefore, it is likely that the expression of these proteins along neuronal development may affect neuronal polarization.MAP1B (Bloom et al., 1995) is the first MAP that is specifically expressed during neural development and that is especially prominent in neurons that are actively extending axons (Calvert and Anderton, 1985;Garner et al., 1990;Fischer and Romano-Clarke, 1991;Ulloa et al., 1993;Black et al., 1994: DiTella et al., 1996Gordon-Weeks and Fischer, 2000). These findings led to the hypothesis that MAP1B might be involved in axon formation by regulating microtubule dynamics. Evidence in favor of this proposal came from antisense experiments showing that MAP1B suppression reduces laminin-promoted axonal elongation (DiTella et al., 1996) and neurite outgrowth in PC12 cells (Brugg et al., 1993). More recently, it was shown that in Drosophila, an MAP1B-like protein is required for proper axonal and dendritic development Roos et al., 2000), and that microscale chromophore-assisted laser inactivation of phosphorylated MAP1B altered growth cone turning behavior in cultured neurons (Mack et al., 2000). The generation of MAP1B mutant mice has also provided additional important information. Despite discrepancies concerning the severity of the effects, all studies demonstrate that MAP1B-deficient mice have an impairment of brain development (Edelman et al., 1996;Takei et al., 1997; GonzalezBillault et...

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Molecular Cloning of Microtubule‐Associated Protein 1 (MAP1A) and Microtubule‐Associated Protein 5 (MAP1B): Identification of Distinct Genes and Their Differential Expression in Developing Brain

Cited by 129 publications

References 55 publications

Identification of the Low Density Lipoprotein (LDL) Receptor-related Protein-1 Interactome in Central Nervous System Myelin Suggests a Role in the Clearance of Necrotic Cell Debris

Identification of the Low Density Lipoprotein (LDL) Receptor-related Protein-1 Interactome in Central Nervous System Myelin Suggests a Role in the Clearance of Necrotic Cell Debris

Mapmodulin/Leucine-rich Acidic Nuclear Protein Binds the Light Chain of Microtubule-associated Protein 1B and Modulates Neuritogenesis

Evidence for the Role of MAP1B in Axon Formation

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