Molecular Cloning of Rat and Porcine Retina-derived POU Domain Factor 1 (POU6F2) from a Pituitary cDNA Library

Abstract

Homeobox transcription factors are known to play crucial roles in the anterior lobe of the pituitary gland. During molecular cloning with the Yeast One-Hybrid System using a 5’-upstream region of the porcine Fshβ as a bait sequence, we have cloned a cDNA encoding a partial sequence of the retina-derived POU domain factor 1 (RPF1) from the porcine pituitary cDNA library and confirmed its specific binding to the bait sequence. In situ hybridization was performed to examine localization of Rpf1 and showed that this gene is expressed in the stem/progenitor cells of the rat pituitary primordium as well as the diencephalon and retina. In addition, real-time PCR demonstrated that Rpf1 transcripts are abundant in early embryonic periods but that this is followed by a decrease during pituitary development, indicating that this factor plays a role in differentiating cells of the pituitary. The transcriptional activity of RPF1 for genes of Prop1, Prrx1 and Prrx2, which were characterized as genes participating in the pituitary stem/progenitor cells by our group, was then examined with full-length cDNA obtained from the rat pituitary. RPF1 showed regulatory activity for Prop1 and Prrx2, but not for Prrx1. These results indicate the involvement of this retina-derived factor in pituitary development.

Whole-Exome Sequencing of Salivary Gland Mucoepidermoid Carcinoma

“…The POU family members are transcriptional regulators, many of which are known to control cell type-specific differentiation pathways(22). POU6F2 is involved in the development of the pituitary(23) and kidney(24). Loss of heterozygosity in regions containing POU6F2 has been reported in Wilms tumor(25).…”

Search for regulatory factors of the pituitary-specific transcription factor PROP1 gene

“…Reporter assays for the responsive region of Group 3 revealed that these factors regulate Prop1 expression through the most distal region, –2993/–1841 b, possessing two SOX2-binding sites. Notably, we demonstrated that RPF1, which was recently characterized in the pituitary [ 13 ], plays a role in PROP1-positive cells in the early pituitary primordium. On the other hand, PITX2 and SOX11 show unique responses in the –1270/–772 b and –443/–155 b regions, respectively.…”

“…Based on the results of the promoter assay, we were interested in RPF1, which was previously demonstrated to be a novel pituitary transcription factor expressed in Rathke’s pouch with a decrease in expression level toward the birth before, and we previously reported that it is a new pituitary transcription factor in the pituitary gland [ 13 ]. Hence, we investigated whether RPF1 and PROP1 coexisted in the early pituitary primordium with in situ hybridization for Rpf1 and immunohistochemistry for PROP1.…”

“…On the other hand, we demonstrated that PROP1 starts its expression in SOX2-positive pituitary stem/progenitor cells and that SOX2 is consistently present in PROP1-positive cells [ 8 ]. In addition, PROP1-positive cells form a stem/progenitor cell niche in the parenchyma of the rat adult anterior lobe [ 9 ], as was elaborated on by further characterizations in subsequent reports [ 10 , 11 , 12 , 13 , 14 ]. PROP1 emerges in SOX2-positive cells early in the rat at embryonic day 11.5 (E11.5) and, after 2 days, occupies all cells in the pituitary primordium of Rathke’s pouch [ 8 ].…”

The association of haplotypes inIGFBP-3gene promoter region and tissue expressions in three pig breeds

“…ARID3A is important for normal embryogenesis, which binds directly to the Oct4, Sox2, Nanog and IgH promoter/enhancer region to activate or repress gene transcription (Webb et al 2011;Popowski et al 2014). PRRX2, also known as S8, is a mesenchymal DNA-binding transcription factor essential for foetal development (Gervais et al 2005), and plays important roles in limb morphogenesis and skeletogenesis (Yoshida et al 2014). Research shows that the highly A-T rich sequence of porcine Fshb promoter region that regulated by PRRX2 and PRRX2 may play a role in suppressing the expression of the Fshb gene (Susa et al 2009).…”