Molecular cloning of red pigment-concentrating hormone (RPCH) from eyestalks of the whiteleg shrimp (Litopenaeus vannamei): Evaluation of the effects of the hormone on ovarian growth and the influence of serotonin (5-HT) on its expression

Chen, Hsiang-Yin; Kang, Bong Jung; Sultana, Zakea; Wilder, Marcy N.

doi:10.1016/j.aquaculture.2018.04.027

Cited by 22 publications

(20 citation statements)

References 36 publications

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“…Previtellogenic female shrimp, with body weights of 48.4 ± 1.6 g, body lengths of 18.3 ± 0.3 cm and a gonadosomatic index (GSI) <0.3%, were applied for artificially induced maturation with unilateral eyestalk ablation and nutrition strength (Chen et al, 2014). Ovarian development was defined into four stages (stage I-IV) based on the classification of predominant oocytes as described previously (Tinikul et al, 2011; Chen H.-Y. et al, 2018).…”

Section: Methodsmentioning

confidence: 99%

Heat Shock Protein 40 (HSP40) in Pacific White Shrimp (Litopenaeus vannamei): Molecular Cloning, Tissue Distribution and Ontogeny, Response to Temperature, Acidity/Alkalinity and Salinity Stresses, and Potential Role in Ovarian Development

Chen

Lin

et al. 2018

Front. Physiol.

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Heat shock proteins (HSPs), a family of conserved proteins that are produced by cells in response to stresses, are known as molecular chaperones with a range of housekeeping and cellular protective functions. The 40 kD heat shock protein (HSP40) is a co-chaperone for HSP70 in the regulation of ATP hydrolysis. Unlike its well-documented cofactor HSP70, little is currently known regarding the biological functions of HSP40 in crustacean species such as penaeid shrimp. In the present study, the cDNA encoding HSP40 (Lv-HSP40) was identified from the Pacific white shrimp Litopenaeus vannamei, a highly significant commercial culture species. The structural organization indicates that Lv-HSP40 belongs to the type-I HSP40s. The muscle, gill, and hepatopancreas are the main sites of Lv-HSP40 transcript expression. Within these tissues, Lv-HSP40 mRNA were predominantly exhibited in the myocytes, epithelial cells and hepatopancreatic cells, respectively. Under acute thermal stress in the culture environment, Lv-HSP40 transcript levels are significantly induced in these three tissues, while low pH stress only upregulates Lv-HSP40 mRNA in the hepatopancreas and gill. During ontogenesis, Lv-HSP40 transcript levels are high at early embryonic stages and drop sharply at late embryonic and early larval stages. The ovary is another major organ of Lv-HSP40 mRNA expression in female shrimp, and Lv-HSP40 transcripts were mainly presented in the follicle cells but only weekly detected in the oocytes. Ovarian Lv-HSP40 mRNA levels increase continuously during gonadal development. Silencing of the Lv-HSP40 gene by RNA interference may effectively delay ovarian maturation after unilateral eyestalk ablation. The roles of Lv-HSP40 in ovarian development are speculated to be independent of its cofactor HSP70, and the vitellogenesis factor vitellogenin (Vg) and vitellogenin receptor (VgR). Our study, as a whole, provides new insights into the roles of HSP40 in multiple physiological processes in L. vannamei: (1) HSP40 is a responding factor during stressful conditions; and (2) HSP40 participates in embryonic and ovarian development.

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Section: Methodsmentioning

confidence: 99%

Heat Shock Protein 40 (HSP40) in Pacific White Shrimp (Litopenaeus vannamei): Molecular Cloning, Tissue Distribution and Ontogeny, Response to Temperature, Acidity/Alkalinity and Salinity Stresses, and Potential Role in Ovarian Development

Chen

Lin

et al. 2018

Front. Physiol.

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“…RPCH has also been shown to have a potentially critical role in reproduction in crustaceans. RPCH transcripts were detected in ovaries and heart in addition to the neural tissue in L. vannamei ( 28 ), supportive of a role for this hormone in reproduction. In vitro co-incubation of ovary explant with nervous tissue caused significant oocyte growth when RPCH was added to the explant culture in several crustacean species ( 38 , 103 , 104 ).…”

Section: Red Pigment-concentrating Hormone (Rpch)mentioning

confidence: 79%

“…Fingerman ( 38 ) further confirmed that the combined effects of RPCH and thoracic ganglia on ovarian maturation was more significant compared to that with explants incubated with thoracic ganglia alone; this suggested that RPCH, as proposed for serotonin, acts as neurotransmitter stimulating the release of VSH, with calcium acting as a second messenger for RPCH. Contrary to the indirect role of RPCH in reproduction, Chen et al ( 28 ) investigated the effects of synthetic RPCH on ovarian maturation in L. vannamei and reported higher vitellogenin mRNA expression in ovaries, increased protein levels in hemolymph, and enlarged oocyte area in treated shrimps ( Figure 4 ); thus, a hypothesis was proposed where RPCH may have a more direct in vitellogenesis. Up-regulation of RPCH in eyestalks following serotonin-induced maturation in the whiteleg shrimp L. vannamei ( 28 ) and crab, S. olivacea ( 66 ) suggested that the downstream roles of serotonin in reproduction are mediated through RPCH.…”

Section: Red Pigment-concentrating Hormone (Rpch)mentioning

confidence: 99%

“…Contrary to the indirect role of RPCH in reproduction, Chen et al ( 28 ) investigated the effects of synthetic RPCH on ovarian maturation in L. vannamei and reported higher vitellogenin mRNA expression in ovaries, increased protein levels in hemolymph, and enlarged oocyte area in treated shrimps ( Figure 4 ); thus, a hypothesis was proposed where RPCH may have a more direct in vitellogenesis. Up-regulation of RPCH in eyestalks following serotonin-induced maturation in the whiteleg shrimp L. vannamei ( 28 ) and crab, S. olivacea ( 66 ) suggested that the downstream roles of serotonin in reproduction are mediated through RPCH. In red swamp crayfish, P. clarkii , RPCH promoted the synthesis and release of methyl farnesoate from the mandibular organs, which in turn regulates maturation ( 101 ).…”

Section: Red Pigment-concentrating Hormone (Rpch)mentioning

confidence: 99%

“…Asterisks indicate significant differences ( P < 0.05) with respect to the control. Reproduced with permission from Chen et al ( 28 ).…”

Section: Red Pigment-concentrating Hormone (Rpch)mentioning

confidence: 99%

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Insights on Molecular Mechanisms of Ovarian Development in Decapod Crustacea: Focus on Vitellogenesis-Stimulating Factors and Pathways

2020

Self Cite

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Vitellogenesis in crustaceans is an energy-consuming process. Though the underlying mechanisms of ovarian maturation in decapod Crustacea are still unclear, evidence indicates the process to be regulated by antagonistically-acting inhibitory and stimulating factors specifically originating from X-organ/sinus gland (XO/SG) complex. Among the reported neuromediators, neuropeptides belonging to the crustacean hyperglycemic hormone (CHH)-family have been studied extensively. The structure and dynamics of inhibitory action of vitellogenesis-inhibiting hormone (VIH) on vitellogenesis have been demonstrated in several species. Similarly, the stimulatory effects of other neuropeptides of the CHH-family on crustacean vitellogenesis have also been validated. Advancement in transcriptomic sequencing and comparative genome analysis has led to the discovery of a large number of neuromediators, peptides, and putative peptide receptors having pleiotropic and novel functions in decapod reproduction. Furthermore, differing research strategies have indicated that neurotransmitters and steroid hormones play an integrative role by stimulating neuropeptide secretion, thus demonstrating the complex intertwining of regulatory factors in reproduction. However, the molecular mechanisms by which the combinatorial effect of eyestalk hormones, neuromediators and other factors coordinate to regulate ovarian maturation remain elusive. These multifunctional substances are speculated to control ovarian maturation possibly via the autocrine/paracrine pathway by acting directly on the gonads or by indirectly exerting their stimulatory effects by triggering the release of a putative gonad stimulating factor from the thoracic ganglion. Acting through receptors, they possibly affect levels of cyclic nucleotides (cAMP and cGMP) and Ca 2+ in target tissues leading to the regulation of vitellogenesis. The “stimulatory paradox” effect of eyestalk ablation on ovarian maturation continues to be exploited in commercial aquaculture operations, and is outweighed by the detrimental physiological effects of this procedure. In this regard, the development of efficient alternatives to eyestalk ablation based on scientific knowledge is a necessity. In this article, we focus principally on the signaling pathways of positive neuromediators and other factors regulating crustacean reproduction, providing an overview of their proposed receptor-mediated stimulatory mechanisms, intracellular signaling, and probable interaction with other hormonal signals. Finally, we provide insight into future research directions on crustacean reproduction as well as potential applications of such research to aquaculture technology development.

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Identification of an additional vitellogenin gene showing hepatopancreas-specific expression in the kuruma prawn Marsupenaeus japonicus

El-Desoky,

Jogatani,

Yamane

et al. 2023

Fish Sci

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Molecular cloning of red pigment-concentrating hormone (RPCH) from eyestalks of the whiteleg shrimp (Litopenaeus vannamei): Evaluation of the effects of the hormone on ovarian growth and the influence of serotonin (5-HT) on its expression

Cited by 22 publications

References 36 publications

Heat Shock Protein 40 (HSP40) in Pacific White Shrimp (Litopenaeus vannamei): Molecular Cloning, Tissue Distribution and Ontogeny, Response to Temperature, Acidity/Alkalinity and Salinity Stresses, and Potential Role in Ovarian Development

Heat Shock Protein 40 (HSP40) in Pacific White Shrimp (Litopenaeus vannamei): Molecular Cloning, Tissue Distribution and Ontogeny, Response to Temperature, Acidity/Alkalinity and Salinity Stresses, and Potential Role in Ovarian Development

Insights on Molecular Mechanisms of Ovarian Development in Decapod Crustacea: Focus on Vitellogenesis-Stimulating Factors and Pathways

Identification of an additional vitellogenin gene showing hepatopancreas-specific expression in the kuruma prawn Marsupenaeus japonicus

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