Molecular cloning of the heat-shock cognate 70 (Hsc70) gene from the two-spotted spider mite, Tetranychus urticae, and its expression in response to heat shock and starvation

Shim, Jooyong; Jung, Duck-Oung; Park, Jae-Woo; Kim, Dongwoo; Ha, Dae-Myung; Lee, Kyeong-Yeoll

doi:10.1016/j.cbpb.2006.07.009

Cited by 45 publications

(30 citation statements)

References 36 publications

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“…The deduced amino acid sequence of DjHsp70 possesses several highly conserved functional motifs (Fig. 1) characteristic of the HSP70/HSC70 family (Shim et al 2006), such as three conserved signatures, the putative ATP-GTP binding motif, the non-organellar consensus motif, and a putative bipartite nuclear localization signal involved in the selective translocation of HSP70 into the nucleus (Knowlton & Salfity 1996). The EEVD sequence at the end of the protein is strictly conserved and shared within the HSP70 family.…”

Section: Discussionmentioning

confidence: 99%

“…Generally, HSP70 is expressed at very low levels under normal condition, but is induced rapidly in response to various stressors. Conversely, HSC70 is actively expressed in normal cells, but does not change in response to stressors (Kiang & Tsokos 1998;Shim et al 2006). However, recent evidence indicates that the general pattern of hsp70/hsc70 gene expression is variable in response to different types and conditions of stressors and also in different stages during development (Lakhotia & Prasanth 2002;Lo et al 2004;Mahroof et al 2005).…”

Section: Introductionmentioning

confidence: 99%

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Cloning and expression analysis of hsp70 gene from freshwater planarian Dugesia japonica

Chen

Lou

et al. 2009

Biologia

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Heat shock protein 70 (HSP70) is an important member of the heat shock protein family. It plays a key role in the process of protecting cells by facilitating the folding of nascent peptides as well as the cellular stress response. We isolated and sequenced a full-length HSP70 cDNA from planarian Dugesia japonica (designated Djhsp70) using rapid amplification of cDNA ends (RACE). Djhsp70 cDNA is highly homologous to other reported hsp70 family genes, and the deduced amino acid sequence shares several eukaryotic HSP70 family motifs. The length of the Djhsp70 ORF from planarian genomic DNA and cDNA was identical, which indicated the absence of introns in the Djhsp70 gene. Semi-quantitative RT-PCR was employed to detect the expression levels of Djhsp70 in response to stressors. Our results indicated that Djhsp70 was an inducible gene, expressed in response to temperature changes, amputation and starvation. Interestingly, the phylogenetic analysis of DjHSP70 supports the idea that planarians belong to a new phylogenetic position -Lophotrochozoans. This is the first molecular analysis of a heat shock protein gene in planarians.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cloning and expression analysis of hsp70 gene from freshwater planarian Dugesia japonica

Chen

Lou

et al. 2009

Biologia

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“…Hsps have been examined in three phytophagous mite species, T. urticae (Shim et al 2006), Tetranychus cinnabarinus (Boisduval) (Li et al 2009), and Panonychus citri (Yang 2011). It was found that expression of Hsp90 and Hsp70-2 in P. citri is induced by shortterm thermal stresses (Yang 2011).…”

Section: Introductionmentioning

confidence: 99%

Cloning and differential expression of five heat shock protein genes associated with thermal stress and development in the polyphagous predatory mite Neoseiulus cucumeris (Acari: Phytoseiidae)

Chen

Zhang

et al. 2015

Exp Appl Acarol

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In order to explore the role of heat shock proteins (Hsps) during thermal stress and development in the predatory mite Neoseiulus cucumeris (Oudemans), we cloned and characterized five full-length Hsp genes. We investigated the expression levels of these genes by quantitative real-time PCR. The five genes characterized here were NcHsp90, NcHsp75, NcHsp70, NcHsp60, and NcHsp40. These Hsps showed high sequence conservation and had greatest identity with heat shock proteins of Metaseiulus occidentalis and other mite and insect species. All five NcHsp genes showed changes in their levels of expression during development. Higher levels of expression were observed in adult females than in adult males, but there were no significant changes between pre-oviposition and post-oviposition stages in the females. NcHsp90, NcHsp75, and NcHsp70 expression levels were up-regulated after a heat shock, and the increases in NcHsp75 and NcHsp70 expression levels were maintained for at least 3 h. Up-regulation of NcHsp60 and NcHsp40 was not detected after 1 h at a high temperature (35-45 °C); however, a significant down-regulation was observed after 3 h heat exposure at 35 °C and 3 h recovery at 25 °C. Cold shock treatment (-5 to 15 °C) for 1 h did not acute elicit changes in the expression levels of any of the genes. At 5 °C, the expression levels of NcHsp90 significantly increased after 6 or 24 h exposure compared to the levels after 1 h exposure. Thus, expression of Hsp genes in N. cucumeris reflected developmental changes, sexual difference, and variable induced response to thermal stress. Increased expression of Hsps might protect N. cucumeris individuals under extreme temperature conditions. Therefore, it may be possible to enhance the thermal tolerance of commercially available N. cucumeris using temperature acclimation. Treatment at 35 °C should be suitable for such acclimation.

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“…There are three characteristic motifs of the Hsp70s from the deduced amino acid sequence: signature 1 (9-16 aa, IDLGTTYS), signature 2 (197-210 aa, DLGGGTFD), and signature 3 (334-348 aa, IVLVGGSTRIPKIQK) by website search (http:// www.expasy.org) (Tabeta and Yamazaki, 2010). The other conserved motifs, such as two consecutive repeats of the tetrapeptide motif GGMP (Luan et al, 2010;Yang et al, 2010), the putative ATP-GTP binding domain, the putative bipartite nuclear location signal, and two cytosolic localization signals (a potential non-organelle eukaryotic consensus motif and the extreme C-terminal domain EEVD) (Shim et al, 2006;Tabeta and Yamazaki, 2010;Xie et al, 2010) were also identified (Fig. 2).…”

Section: Cloning and Sequence Analysis Of Turtle Thsc70mentioning

confidence: 99%

“…Recent evidence indicates that Hsc70 gene expression pattern varies in response to different stresses and development (Shim et al, 2006). A number of Hsc70s have been identified from such species as human, mouse, two-spotted spider mite, zebrafish, and tiger shrimp (Santacruz et al, 1997;Hunt et al, 1999;Lo et al, 2004;Shim et al, 2006;Brocchieri et al, 2008) that have helped us to better understand their phylogenetic relationship and explore their biological functions. However, there are still many Hsc70 molecules that remain unidentified so far in less popular animal species, such as turtles and snakes.…”

Section: Introductionmentioning

confidence: 99%

Identification of a heat shock cognate protein 70 gene in Chinese soft-shell turtle (Pelodiscus sinensis) and its expression profiles under thermal stress

Kang

Zhang

et al. 2012

J. Zhejiang Univ. Sci. B

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Abstract:The heat shock cognate protein 70 (Hsc70) is a member of a 70-kDa heat shock protein (HSP70) family that functions as molecular chaperones. In this study, a novel Hsc70 gene from Chinese soft-shelled turtle (Pelodiscus sinensis) (tHsc70) was identified. The tHsc70 full-length complementary DNA (cDNA) is 2 272 bp long with a 1 941-bp open reading frame (ORF) encoding 646 amino acids. Three characteristic signature regions of the HSP70 family, two major domains of an adenosine triphosphate (ATP)/guanosine triphosphate (GTP) binding domain (ABD), and a substrate-binding domain (SBD) were present in the predicted tHsc70 amino acid sequence. The tHsc70 gene was expressed in Escherichia coli BL21 and the expression product reacted with the anti-Hsc70 mouse monoclonal antibody by Western blotting. Homology analysis revealed that tHsc70 shared identity from 53.9% to 87.7% at the nucleotide level, and 49.1% to 99.5% at the amino acid level with the known Hsc70s. Phylogenetic analysis showed that tHsc70 was clustered together with the Hsc70 gene of another reptile species (Alligator mississippiensis). The tHsc70 was expressed in the liver, lung, heart, and skeletal muscle. The expression patterns of tHsc70 messenger RNA (mRNA) differed among different tissues under different durations of heat stress at 40 °C. Adaptation at 25 °C for 1 h after heat stress was also different among tissues and length of heat stress. Irrespective of different profiles of expression under heat stress, tHsc70 may play roles in protecting turtles from thermal stress.

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Molecular cloning of the heat-shock cognate 70 (Hsc70) gene from the two-spotted spider mite, Tetranychus urticae, and its expression in response to heat shock and starvation

Cited by 45 publications

References 36 publications

Cloning and expression analysis of hsp70 gene from freshwater planarian Dugesia japonica

Cloning and expression analysis of hsp70 gene from freshwater planarian Dugesia japonica

Cloning and differential expression of five heat shock protein genes associated with thermal stress and development in the polyphagous predatory mite Neoseiulus cucumeris (Acari: Phytoseiidae)

Identification of a heat shock cognate protein 70 gene in Chinese soft-shell turtle (Pelodiscus sinensis) and its expression profiles under thermal stress

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