Molecular Cloning of the m-<I>Golsyn</I> Gene and its Expression in the Mouse Brain

Abstract

The mouse ortholog of the human GOLSYN gene, termed the m-Golsyn gene, was isolated and mapped to the region on mouse chromosome 15B3.2 syntenic with human chromosome 8q23. Three mRNA species (type la, 1b, and type 2) were produced by use of alternative transcription initiation points and alternative splicing events. The type 1 mRNAs were expressed only in the brain, whereas the type 2 was detected in various tissues. m-Golsyn protein was expressed in various tissues including the brain. Immunohistochemical study of m-Golsyn protein showed its prominent expression in the neuronal cells in various regions of the brain and strong expression in the choroid plexus ependymal cells lining the ventricles. m-Golsyn protein was found to be homologous to syntaphilin, a regulator of synaptic vesicle exocytosis. These results indicate that the m-Golsyn protein may play an important role in intracellular protein transport in neuronal cells of the brain.

High-Level Expression of Golsyn/Syntabulin in Glandular Epithelium and its Role in the Secretory Process~!2010-01-02~!2010-06-13~!2010-08-16~!

“…Earlier, we demonstrated that Golsyn/Syntabulin is ubiquitously expressed in various mouse organs including the brain at mRNA and protein levels [16,17]. In the brain, nerve cells, but not glial cells, express Golsyn/Syntabulin; however, the cell types expressing Golsyn/Syntabulin in the organs other than the brain remained unclear.…”

“…2D and E). Golsyn/Syntabulin has been reported to be ubiquitously expressed in nerve cells of various brain regions of postnatal and adult mice [16,17]. It thus appears that this protein plays a significant role in nerve cells of the periphery in addition to those of the brain.…”

“…Immunoblot analysis was performed as described previously [16]. Briefly, proteins separated by SDS-PAGE on 7.5% of slab gels were transferred electrophoretically for 75 minutes at 100V onto a polyvinylidene difluoride membrane (Millipore) in Transfer buffer (25 mM Tris, 192 mM glycine, 10% methanol).…”

“…Sections of mouse organs were prepared as described earlier [16]. Briefly, animals were perfused via the left ventricle of the heart with saline, under anesthesia by an intraperitoneal injection of pentobarbital (250 mg/kg, i.p.…”

“…Later we isolated m-Golsyn, the mouse ortholog of human GOLSYN, and mapped it to the region on mouse chromosome 15B3.2 syntenic with human chromosome 8q23. m-Golsyn was expressed not only in neuronal cells but also in various organs and cell lines including epithelial cells [16,17]. Golsyn/Syntabulin, therefore, may be involved in vesicular transport in various organs other than the brain.…”