Molecular cloning, phylogenetic analysis and developmental expression of a vitellogenin (Vg) gene from the intertidal copepod Tigriopus japonicus

Lee, Kyun-Woo; Hwang, Dae‐Sik; Rhee, Jae-Sung; Ki, Jang‐Seu; Park, Heum Gi; Ryu, Jae‐Chun; Raisuddin, Sheikh; Lee, Jae‐Seong

doi:10.1016/j.cbpb.2008.04.009

Cited by 46 publications

(29 citation statements)

References 41 publications

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“…Indeed, GfVtg gene expression was from 10 2 to 10 3 times greater in females. These results agree with those recently reported by Lee et al (2008), which showed that, in the copepod Tigriopus japonicus, females expressed 270 times more Vtg than males did. Previously, Sagi et al (1999) had also shown that secondary vitellogenic females exhibited levels of vitellogenetic-specific proteins 10 3 times higher than in males, in the crayfish Cherax quadricarinatus, whereas Shechter et al (2005), using real-time PCR, detected no evidence of expression of the Vtg gene in males of this species.…”

Section: Vitellogenin Gene Expression In Control Gammaridssupporting

confidence: 95%

“…However, as underlined by Ford (2008), nearly all the studies related to Vtg measurements in crustaceans following exposure to environmental contaminants (both in the field and in the lab) mainly focused on females (e.g. Gagné and Blaise 2004;Gagné et al 2005;Ghekiere et al 2006b;Huang et al 2006;Lee et al 2008;Martin-Diaz et al 2004;Oberdorster et al 2000;Poynton et al 2007;Volz et al 2003) and less on males and juveniles (e.g. Billinghurst et al 2000;Sanders et al 2005).…”

Section: Introductionmentioning

confidence: 99%

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Vitellogenin-like gene expression in freshwater amphipod Gammarus fossarum (Koch, 1835): functional characterization in females and potential for use as an endocrine disruption biomarker in males

Xuereb¹,

Bezin

Chaumot³

et al. 2011

Ecotoxicology

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The induction of vitellogenin (Vtg) synthesis is widely accepted as a biomarker of estrogenic exposure in male and juvenile fish. Vtg synthesis has emerged as an interesting endpoint to assess endocrine disruptor (ED) effects in crustaceans. However, studies reporting induction of Vtg in male crustaceans are lacking. This study investigated the expression of the Vtg gene in a freshwater amphipod, Gammarus fossarum, using calibrated real-time reverse transcription polymerase chain reaction (real-time RT PCR). First, we described the basal pattern of expression in healthy male and female organisms at different reproductive moult stages, in order to validate the function of this gene. Females expressed from 200 to 700 times more Vtg transcripts than males, depending on the female reproductive stage. Females displayed significant elevation of Vtg mRNA levels at the end of the inter-moult phase and at the beginning of the pre-moult phase. Second, male gammarids were exposed to the estrogenic compound nonylphenol (NP) (0.05, 0.5, 5 and 50 μg L(-1)) and to the anti-androgen cyproterone (1, 10, 100 and 1000 μg L(-1)) for 2, 4, 8 and 16 days. Both chemicals altered the pattern of interindividual variability of Vtg gene expression in males with strong induction in some individuals. Finally, the impact of urban wastewater treatment plants (WWTP) on male Vtg gene expression was assessed in organisms transplanted in the field during in situ bioassay campaigns in three different watersheds. Induction of the Vtg mRNA level was observed in males transplanted downstream from WWTP effluent discharge in two of the three study sites.

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Section: Vitellogenin Gene Expression In Control Gammaridssupporting

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Vitellogenin-like gene expression in freshwater amphipod Gammarus fossarum (Koch, 1835): functional characterization in females and potential for use as an endocrine disruption biomarker in males

Xuereb¹,

Bezin

Chaumot³

et al. 2011

Ecotoxicology

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“…The results showed that the expression of the Vg gene was not completely silent in males and that strong differences in Vg gene expression were observed between males and females (p < 0.0001). These results confirm the baseline level of Vg in male crustaceans as reported in the copepod Tigriopus japonicus (Lee et al 2008) or in the amphipod Gammarus fossarum (Jubeaux et al 2012). Moreover, no significant difference (p > 0.05) was detected between the Vg gene expression measured in CLD-exposed and control males after 90 days of exposure, although a trend of increase was observed after 240 days of exposure (Fig.…”

Section: Expression Of the Vitellogenin Genesupporting

confidence: 89%

Vitellogenin and vitellogenin receptor gene expression and 20-hydroxyecdysone concentration in Macrobrachium rosenbergii exposed to chlordecone

Lafontaine

Hanikenne

Boulangé-Lecomte

et al. 2016

Environ Sci Pollut Res

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Chlordecone is a persistent organochlorine pesticide widely used in Guadeloupe (French West Indies) to control the banana weevil Cosmopolites sordidus. Although it was previously highlighted that chlordecone may affect the reproduction and growth of vertebrate species, little information is available on the chlordecone effects in invertebrates. The present study investigated the effects of chlordecone on a hormone and a protein having key roles in reproduction and growth of the decapod crustacean Macrobrachium rosenbergii, by measuring the 20-hydroxyecdysone concentration, vitellogenin, and vitellogenin receptor gene expression, as well as the bioconcentration of chlordecone in exposed prawns. First, the results revealed that chlordecone was accumulated in M. rosenbergii. Then, it was found that Vg and VgR gene expression were increased in male and female M. rosenbergii exposed to chlordecone for 90 and 240 days, while the 20-hydroxyecdysone concentrations were decreased. This work suggests that chlordecone accumulates in prawn tissues and could affect key molecules involved in the reproduction and the growth of the invertebrate M. rosenbergii. However, many questions remain unresolved regarding the impacts of chlordecone on growth and reproduction and the signaling pathways responsible for these effects, as well as the potential role of confounding factors present in in situ studies.

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“…The re sulting increase in salmon lice abundance causes major economic losses in aquaculture and also represents a threat to wild salmon populations (Bjorn et al 2001). The reproductive system of salmon louse has been described morphologically (Ritchie et al 1996), but few molecular studies of reproduction in salmon louse and the associated copepod group have been published (Todd et al 2005, Lee et al 2008, Dalvin et al 2009, Hwang et al 2009). …”

Section: Introductionmentioning

confidence: 99%

Characterisation of two vitellogenins in the salmon louse Lepeophtheirus salmonis: molecular, functional and evolutional analysis

Dalvin¹,

Frost²,

Loeffen³

et al. 2011

Dis. Aquat. Org.

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The salmon louse Lepeophtheirus salmonis Krøyer affects a variety of wild salmonoid hosts, but is also an important pest in aquaculture, which is a globally important and rapidly growing industry. Salmon lice have large reproductive outputs, and knowledge of reproductive processes may be crucial for the control of this parasite. Here, we report on the characterisation of 2 vitellogenins (LsVit1 and LsVit2), which are the precursors of salmon-louse egg-yolk glycoprotein. The structure of LsVit1 and LsVit2 was examined and compared to that in other oviparous animals. Phylogenetic analysis of LsVit1 and LsVit2 confirmed the view that crustaceans are a polyphyletic group. Transcriptional and translational analysis demonstrated production of LsVit1 and LsVit2 in the subcuticular tissue of the adult female lice. LsVit1 and LsVit2 could also be found in maturing oocytes and developing embryos and early larval stages. LsVit2 was found to be processed into 2 smaller fragments, whereas LsVit1 was found to be full length when deposited into the oocytes. Degradation of LsVit1 and LsVit2 was characterised through embryogenesis and the early non-feeding larval stages. Finally, protein content and the level of free amino acids were analysed in embryos and larval stages and their role in nutrition and osmoregulation discussed. In conclusion, our results confirm the role of vitellogenins in reproduction as providers of embryonic and larval nutrition.

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Molecular cloning, phylogenetic analysis and developmental expression of a vitellogenin (Vg) gene from the intertidal copepod Tigriopus japonicus

Cited by 46 publications

References 41 publications

Vitellogenin-like gene expression in freshwater amphipod Gammarus fossarum (Koch, 1835): functional characterization in females and potential for use as an endocrine disruption biomarker in males

Vitellogenin-like gene expression in freshwater amphipod Gammarus fossarum (Koch, 1835): functional characterization in females and potential for use as an endocrine disruption biomarker in males

Vitellogenin and vitellogenin receptor gene expression and 20-hydroxyecdysone concentration in Macrobrachium rosenbergii exposed to chlordecone

Characterisation of two vitellogenins in the salmon louse Lepeophtheirus salmonis: molecular, functional and evolutional analysis

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